Chemical Composition and Antimicrobial Properties of Honey Bee Venom

Abstract

Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In this work, the composition of volatile and extractive components of dry and fresh bee venom (BV) was determined by GC-MS, as well as antimicrobial activity against seven types of pathogenic microorganisms. One-hundred and forty-nine organic C1–C19 compounds of different classes were found in the volatile secretions of the studied BV samples. One-hundred and fifty-two organic C2–C36 compounds were registered in ether extracts, and 201 compounds were identified in methanol extracts. More than half of these compounds are new to BV. In microbiological tests involving four species of pathogenic Gram-positive and two species of Gram-negative bacteria, as well as one species of pathogenic fungi, the values of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined for samples of dry BV, as well as ether and methanol extracts from it. Gram-positive bacteria show the greatest sensitivity to the action of all tested drugs. The minimum MIC values for Gram-positive bacteria in the range of 0.12–7.63 ng mL−1 were recorded for whole BV, while for the methanol extract they were 0.49–125 ng mL−1. The ether extracts had a weaker effect on the tested bacteria (MIC values 31.25–500 ng mL−1). Interestingly, Escherichia coli was more sensitive (MIC 7.63–500 ng mL−1) to the action of bee venom compared to Pseudomonas aeruginosa (MIC ≥ 500 ng mL−1). The results of the tests carried out indicate that the antimicrobial effect of BV is associated with the presence of not only peptides, such as melittin, but also low molecular weight metabolites.

Natural Sources of Therapeutic Agents Used in Skin Conditions

“…BV is a defense tool of the hive against predators and is secreted by a gland located in the abdominal cavity of bees (Apis mellifera) [31,32]. For thousands of years, people have used bee products in various forms to treat diseases, with the curative effects being mentioned in the Holy Quran, the Bible, and the Veda [33]. BV was used medically in ancient Egypt and Greece, and in China, BV therapy has been known and applied for about 5000 years [33].…”

“…For thousands of years, people have used bee products in various forms to treat diseases, with the curative effects being mentioned in the Holy Quran, the Bible, and the Veda [33]. BV was used medically in ancient Egypt and Greece, and in China, BV therapy has been known and applied for about 5000 years [33]. Historically, BV has generally been associated with the treatment of inflammatory conditions, rheumatism, and skin diseases, as well as malaria [34,35].…”

Bee venom: A potential natural alternative to conventional preservatives for prolonging the shelf-life of soft cheese ‘Talaga’

“…In various studies, honey-BV has been identified for its antimicrobial properties against both bacteria and fungi [ 21 , 39 , 40 ]. Furthermore, recent research suggests that honey-BV possesses antimicrobial properties that could potentially be utilized in food preservation [ 41 ]. Therefore, using honeybee venom as a food preservative is a relatively novel concept that has gained some attention in recent years.…”

Chemical Composition of Volatile and Extractive Components of Canary (Tenerife) Propolis

“…In recent years, increasing attention has been paid to propolis as an antidote to human microbial pathogens. In terms of antisepticity, propolis stands out among most other beekeeping products, second only to bee venom in terms of potency [6][7][8]. It exhibits a wide range of medicinal properties, which are not limited to antimicrobial action, as reflected in a number of recent comprehensive reviews [9][10][11][12].…”