Melittin antimicrobial peptide thin layer on bone implant chitosan-antibiotic coatings and their bactericidal properties

Zarghami, Vahid; Ghorbani, Mohammad; Bagheri, Kamran Pooshang; Shokrgozar, Mohammad Ali

doi:10.1016/j.matchemphys.2021.124432

Cited by 30 publications

(20 citation statements)

References 44 publications

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“…The compounds bind through weak intermolecular interactions, which allows easy release of the antibiotic and, consequently, the fight against bacteria. 121…”

Section: Chitosan As a Scaffold Surface Modifiermentioning

confidence: 99%

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

Kołakowska

Gadomska‐Gajadhur

Ruśkowski³

2021

Preprint

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The article focuses on a polysaccharide of natural origin – chitosan and its application in tissue engineering. The preparation process and physicochemical properties of the saccharide are described. The degradation of chitosan and the properties influencing the process both outside and in living organism were examined. Four applications in bone tissue engineering can be distinguished: preparation of cell scaffolds exclusively from chitosan, from a chitosan composite or from a chitosan polyelectrolyte complex. The fourth way is to modify the surface of scaffolds made of other materials by covering them with a layer of chitosan. At the end of the article, the processes taking place after placing the implant inside the body are described, how the structure of chitosan affects the behaviour of bone cells in the adhesion process and life processes.

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“…The compounds bind through weak intermolecular interactions, which allows easy release of the antibiotic and, consequently, the fight against bacteria. 121…”

Section: Chitosan As a Scaffold Surface Modifiermentioning

confidence: 99%

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

Kołakowska

Gadomska‐Gajadhur

Ruśkowski³

2021

Preprint

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“…Accordingly, it has been found that the combination of antimicrobial peptides (AMPs) with conventional antibiotics can be a promising strategy against MDR bacterial pathogens ( Zusman et al, 2013 ; Schmid et al, 2019 ). Currently, AMPs are found as novel developing antibacterial agents that could serve as an alternative to antibiotics ( Bardbari et al, 2018 ; Memariani et al, 2018 ; Aghazadeh et al, 2019 ; Akbari et al, 2019 ; Bevalian et al, 2021 ; Zarghami et al, 2021a ). Furthermore, the application of AMPs along with conventional antibiotics usually enhances the results of mono drug therapies ( Bardbari et al, 2018 ; Akbari et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Highly Synergistic Effects of Melittin With Vancomycin and Rifampin Against Vancomycin and Rifampin Resistant Staphylococcus epidermidis

et al. 2022

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Methicillin-resistant Staphylococcus epidermidis (MRSE) strains are increasingly emerging as serious pathogens because they can be resistant to many antibiotics called multidrug resistance (MDR) that limit the therapeutic options. In the case of vancomycin- and rifampin-resistant MDR-MRSE, the physicians are not allowed to increase the doses of antibiotics because of severe toxicity. Accordingly, we investigated the synergistic activity of melittin antimicrobial peptide with vancomycin and rifampin against vancomycin-resistant, and rifampin-resistant MDR-MRSE isolates. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration index (FICi), and fractional bactericidal concentration index (FBCi) of antimicrobial agents against isolates were determined. Coagulate activities and serum and salt stability as well as melittin cytotoxicity on the human embryonic kidney (HEK) 293 cells and human red blood cells (RBCs) at their synergistic concentrations. MIC and MBC values for melittin were in the range of 0.312–2.5 and 0.312–5, respectively. Results also showed that the interaction of melittin with drugs was highly synergistic in which the geometric means of FICi and FBCi were < 0.5. Induced synergism led to a decrease in melittin, rifampin, and vancomycin concentrations by 8–1,020, 2–16, and 4–16-folds, respectively. This phenomenon caused a reduction in melittin toxicity by which the synergistic concentration of melittin needed to kill bacteria did not show cytotoxicity and hemolytic activity. Besides, no coagulation activity was found for the synergistic and alone concentrations of melittin in both Prothrombin Time (PT) and Partial Thromboplastin Time (PTT). Interestingly, the antibacterial activity of melittin in Mueller Hinton Broth (MHB) containing human serum did no significant differences between MIC and MBC values of melittin in MHB and MHB containing 10% human serum. The present findings showed that the therapeutic index of melittin was improved by 32.08- and 12.82-folds when combined with vancomycin and rifampin, respectively. Taken together, the obtained data show that melittin alone was effective against MDR-MRSE isolates and this antimicrobial peptide showed highly synergistic effects with vancomycin and rifampin without causing toxicity. Therefore, the combination of melittin and traditional antibiotics could be a promising strategy for the treatment of infections caused by MDR-MRSE.

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“…Melittin, an antimicrobial peptide of 26 amino acid residues, is the main component of honey bee venom with potent antibacterial activity [ 28 – 30 ]. Melittin induces pores in membranes and has a suitable synergistic effect with antibiotics on killing the resistant bacteria and, also, inhibiting biofilm formation [ 23 , 28 , 31 – 34 ].…”

Section: Introductionmentioning

confidence: 99%

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Zarghami

Ghorbani

Bagheri

et al. 2022

J Mater Sci: Mater Med

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Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants. The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection. Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate. Melittin was then coated on composite coatings by drop casting method. The surfaces were analyzed by FTIR, XRD, and SEM instruments. Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity. Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings. Melittin increased the proliferation of MC3T3 cells. Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria. In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.

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Melittin antimicrobial peptide thin layer on bone implant chitosan-antibiotic coatings and their bactericidal properties

Cited by 30 publications

References 44 publications

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

Biomimetic scaffolds based on chitosan in bone regeneration. A review.

Highly Synergistic Effects of Melittin With Vancomycin and Rifampin Against Vancomycin and Rifampin Resistant Staphylococcus epidermidis

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

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