Melissa officinalis extract induces apoptosis and inhibits proliferation in colon cancer cells through formation of reactive oxygen species

Weidner, Christopher; Rousseau, Morten; Plauth, Annabell; Wowro, Sylvia J.; Fischer, Cornelius; Abdel-Aziz, H; Sauer, Sascha

doi:10.1016/j.phymed.2014.12.008

Cited by 66 publications

(56 citation statements)

References 28 publications

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“…Genistein also caused the cell cycle arrest in G2/M phase, which was accompanied by activation of the CDKN1A, CDKN1C, CDKN2A, CDKN2B, CDKN2C, and GADD45A protein expression, as described in [209]. Ethanolic extract from the leaves of lemon balm ( Melissa officinalis ) was shown to exhibit the anti-proliferative effects on human colon carcinoma HT-29 and T84 cells through a G2/M cell cycle arrest and reduced expression of CDK-2, -4, and -6, and cyclin D3, and induced expression of CDKN2C (p18) and CDKN1A (p21) proteins [210]. The ethanol extract from Tetrastigma hemsleyanum was found to induce the cell cycle arrest in S phase in hepatocellular carcinoma HepG2 cells associated with decreasing expression of CDK1, and downregulating of cyclin A-CDK1 complex [211].…”

Section: Cell Cycle Regulation By Natural Compounds Affecting Cyclinsmentioning

confidence: 99%

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

et al. 2017

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Natural compounds from various plants, microorganisms and marine species play an important role in the discovery novel components that can be successfully used in numerous biomedical applications, including anticancer therapeutics. Since uncontrolled and rapid cell division is a hallmark of cancer, unraveling the molecular mechanisms underlying mitosis is key to understanding how various natural compounds might function as inhibitors of cell cycle progression. A number of natural compounds that inhibit the cell cycle arrest have proven effective for killing cancer cells in vitro, in vivo and in clinical settings. Significant advances that have been recently made in the understanding of molecular mechanisms underlying the cell cycle regulation using the chemotherapeutic agents is of great importance for improving the efficacy of targeted therapeutics and overcoming resistance to anticancer drugs, especially of natural origin, which inhibit the activities of cyclins and cyclin-dependent kinases, as well as other proteins and enzymes involved in proper regulation of cell cycle leading to controlled cell proliferation.

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Section: Cell Cycle Regulation By Natural Compounds Affecting Cyclinsmentioning

confidence: 99%

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

et al. 2017

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“…Its association with severe forms of cardiomyopathy and/or congestive heart failure in cancer patients greatly limits DOX application [1–2]. Cellular damage induced by DOX is mediated by the oxidative damage of cardiomyocytes, plasma membranes and the consequent death of those cells by apoptosis [2–3].…”

Section: Introductionmentioning

confidence: 99%

“…Normally, herbal tea of MO is used for its aromatic, digestive and antispasmodic properties and to reduce gastrointestinal disorders and sleep disturbance [10–11]. Moreover, MO has been reported to show potent anti-tumor effects in a variety of human cancer cell lines [12–14] and to induce apoptosis in colon carcinoma cells through formation of reactive oxygen species (ROS) [1]. Caffeic acid, protocatechuic acid, rosmarinic acid, ferulic acid, and syringic acid have been reported as the most abundant phenolic compounds in MO [15].…”

Section: Introductionmentioning

confidence: 99%

Melissa officinalis Protects against Doxorubicin-Induced Cardiotoxicity in Rats and Potentiates Its Anticancer Activity on MCF-7 Cells

et al. 2016

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Cardiotoxicity is a limiting factor of doxorubicin (DOX)-based anticancer therapy. Due to its beneficial effects, we investigated whether standardized extract of Melissa officinalis (MO) can attenuate doxorubicin-induced cardiotoxicity and can potentiate the efficacy of DOX against human breast cancer cells. MO was administered orally to male albino rats once daily for 10 consecutive days at doses of 250, 500 and 750 mg/kg b.wt. DOX (15 mg/kg b.wt. i.p.) was administered on the 8th day. MO protected against DOX-induced leakage of cardiac enzymes and histopathological changes. MO ameliorated DOX-induced oxidative stress as evidenced by decreasing lipid peroxidation, protein oxidation and total oxidant capacity depletion and by increasing antioxidant capacity. Additionally, MO pretreatment inhibited inflammatory responses to DOX by decreasing the expressions of nuclear factor kappa-B, tumor necrosis factor-alpha and cyclooxygenase-2 and the activity of myeloperoxidase. MO ameliorated DOX-induced apoptotic tissue damage in heart of rats. In vitro study showed that MO augmented the anticancer efficacy of DOX in human breast cancer cells (MCF-7) and potentiated oxidative damage and apoptosis. Thus, combination of DOX and MO may prove future cancer treatment protocols safer and more efficient.

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“…Induction of apoptosis in DU-145 cells after extract and synthetic phenolic compounds treatments Apoptosis is accepted as a natural defense mechanism against cancers. It is evident that plants and their phytochemicals induces apoptosis and inhibits proliferation of cancer cells, showing significant anticancer activities (Mei et al, 2016, Weidner et al, 2015. To reveal which type of cell death induced after S. pilifera extract treatments, we used Annexin V/7ADD double staining assay.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Cytotoxic Effect of Salvia pilifera Extracts and Synthetic Chlorogenic and Caffeic Acids on DU145 Prostate Cancer Cells Line

Yumrutaş¹,

Pehlivan²,

Güven³

et al. 2018

Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi

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Salvia species have been used in the treatment of many diseases due to their medical effects, and the effects of these species on prostate cancer cells should be investigated in more detail. In this study, we aimed to determine anti-carcinogenic activities of dichloromethane (DCM) and methanol (MeOH) extracts of Salvia pilifera and the synthetic chloregenic (CGA) and caffeic acids (CA) on DU-145 prostate cancer cells. The cytotoxicity of extracts and synthetic compounds on cell viability of DU-145 was measured by using MTT method. Induction of apoptosis was tested by using Annexin V and 7ADD staining. DNA fragmentation was evaluated in cells. Also, transcripton levels of Bax, Caspase 3, Caspase 9 and Bcl-2 and Bcl-xL genes were determined. Lastly, the phenolic compounds in MeOH extract were determined by HPLC. In MTT test, extracts, CGA and CA were found to be diminished proliferation of DU145 cells. However, in apoptosis assay, no apoptotic activity for extract and synthetic compounds was observed. In DNA fragmentation test, while no significant difference in extracts group was observed as compared to controls, fragmentation as swab in synthetic compound groups was observed. Small changes were observed in transcription levels of apoptotic and antipoptotic genes. A total of 11 phenolic acids were determined including fumaric acid, gallic acid, gallocatechin, catechin, oleorufein, 4-hydroxybenzoic acid, caffeic acid, syringic acid, ellagic acid, 3-hydroxy cinnamic acid and protocatechuic acid. Results of the present study suggest that S. pilifera extracts and synthetic CGA and CA might have cytotoxic effects on DU145 cell at certain concentration (≥50 µg ml -1 for DCM; ≥ 100 µg ml -1 for MeOH; ≥ 1 µg ml -1 for CGA and CA) yet that these effects may be manifested through another pathway but apoptosis.

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Melissa officinalis extract induces apoptosis and inhibits proliferation in colon cancer cells through formation of reactive oxygen species

Cited by 66 publications

References 28 publications

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

Melissa officinalis Protects against Doxorubicin-Induced Cardiotoxicity in Rats and Potentiates Its Anticancer Activity on MCF-7 Cells

Investigation of Cytotoxic Effect of Salvia pilifera Extracts and Synthetic Chlorogenic and Caffeic Acids on DU145 Prostate Cancer Cells Line

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