Melibiose, Difructose Anhydride III and Difructose Anhydride IV Enhance Net Calcium Absorption in Rat Small and Large Intestinal Epithelium by Increasing the Passage of Tight Junctions In Vitro

Abstract

An Ussing chamber technique was used to determine the effects of three indigestible disaccharides on net Ca transport from the luminal side to the basolateral side of isolated preparations of jejunal, ileal, cecal and colonic epithelium in rats. Permeability of Lucifer Yellow (LY) and transepithelial electrical resistance (TEER), which are indicators of intercellular passage of the intestinal mucosa, were also determined. The concentrations of Ca in the serosal and mucosal media were 1.25 mmol/L and 10 mmol/L, respectively. After a 30-min incubation, the net Ca transport, LY passage and TEER were determined. In the control experiment, LY permeability was lowest, and TEER value was highest in the colon. The addition of 1-100 mmol/L melibiose, difructose anhydride (DFA)III, or DFAIV to the mucosal medium increased the net Ca absorption and LY permeability dose-dependently in the jejunum, ileum, cecum and colon preparations. Melibiose decreased TEER dose-dependently in the jejunum and cecum, but not in the ileum and colon. DFAIII decreased TEER dose-dependently in the jejunum, cecum and colon, but not in the ileum. DFAIV decreased TEER dose-dependently in all four intestinal portions. Positive linear relationships were found between net Ca transport and LY passage in all portions of the intestine, whereas negative linear relationships were found between net Ca absorption and TEER. We concluded that the three indigestible saccharides directly affect the epithelial tissue and activate the passage of tight junctions, thereby promoting Ca absorption in the small and large intestine in vitro.

Effect of Difructose Anhydride III on Calcium Absorption in Humans

“…Dose-dependent enhancement by DFAIII of calcium excretion has also been demonstrated by the Ussing chamber technique. 4) It is therefore intriguing that the results obtained from different types of experiment are very similar in respect of dose-dependent enhancement.…”

“…It has been demonstrated by the Ussing chamber technique with rats that the calcium absorption in all gastrointestinal areas was dose-dependently stimulated by DFAIII. 4) It has been also clarified that the mechanism for such stimulation involved active transport through the tight junction of the enteric canal. 4) However, it is unclear whether the stimulation of calcium absorption in the present study was caused by the same mechanism or not.…”

“…4) It has been also clarified that the mechanism for such stimulation involved active transport through the tight junction of the enteric canal. 4) However, it is unclear whether the stimulation of calcium absorption in the present study was caused by the same mechanism or not. Meanwhile, we compared the urinary calcium excretion after an intake of DFAIII with that of fructooligosaccharide, whose stimulatory effect on calcium absorption has been demonstrated.…”

“…It has been demonstrated DFAIII increased calcium absorption in in vivo balance study with nomal and ovariectomized rats and reveals by the Ussing-type chamber technique with the rats isolated intestinal mucosa that DFAIII increased calcium absorption through the paracellular route in the small and large intestine. [4][5][6][7] However, DFAIII has not been demonstrated as a special food valuable for calcium absorption in humans. We examine here the effects of DFAIII intake on calcium absorption in healthy male adults that were chosen to eliminate any influence of sexual cyclerelated hormonal changes.…”

Sugarcane yeast inclusion for broilers at post-hatch

“…The growing broilers need to deposit higher levels of protein and minerals in the carcass to ensure maximum muscle and bone growth. The use of oligosaccharides improves the intestinal absorption of minerals in rats and humans (Ohta et al, 1998;Mineo et al, 2002); however, this effect has been poorly studied in birds (Oliveira et al, 2009). In this study, a greater body retention of minerals was not observed, possibly because whole yeast cell was used instead of isolated MOS, and lower concentrations of MOS are found in the whole yeast cell.…”