Melatonin regulates the development and function of bovine Sertoli cells via its receptors MT1 and MT2

Yang, Wenguang; Tang, Kai-Fu; Fu, Changzhen; Riaz, Hasan; Zhang, Qiong; Liu, Zan

doi:10.1016/j.anireprosci.2014.03.017

Cited by 67 publications

(68 citation statements)

References 40 publications

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“…In our study, we found that the melatonin receptors MT1 and MT2 in the goat seminiferous tubule were increased during the breeding season, indicating that melatonin during the breeding season increased and influenced the process of spermatogenesis. Meanwhile, many studies have shown that melatonin receptors are expressed in spermatozoa and spermatocytes [35–37]. However, we found that melatonin receptors are expressed in almost every subtype of spermatogenesis cells in dairy goats.…”

Section: Discussionmentioning

confidence: 58%

“…Sertoli cells can secrete several factors, such as GDNF, fibroblast growth factor-2 (FGF2), bone morphogenetic protein 4 (BMP4) and so on, all of which are related to spermatogenesis [36]. Recent reports have shown that exogenous melatonin acts via its receptors and appears to play regulatory roles in the development and function of bovine Sertoli cells [37]. In our study, ELISA showed that the GDNF concentration was increased in the melatonin added group, indicating that melatonin can promote Sertoli cell produced GDNF.…”

Section: Discussionmentioning

confidence: 99%

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Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

Niu

et al. 2016

Oncotarget

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Melatonin has been reported to be an important endogenous hormone for regulating neurogenesis, immunityand the biological clock. Recently, the effects of melatonin on neural stem cells (NSCs), mesenchymal stem cells(MSCs), and induced pluripotent stem cells(iPSCs) have been reported; however, the effects of melatonin on spermatogonia stem cells (SSCs) are not clear. Here, 1μM and 1nM melatonin was added to medium when goat SSCs were cultured in vitro, the results showed that melatonin could increase the formation and size of SSC colonies. Real-time quantitative PCR (QRT-PCR) and western blot analysis showed that the expression levels of SSC proliferation and self-renewal markers were up-regulated. Meanwhile, QRT-PCR results showed that melatonin inhibit the mRNA expression level of SSC differentiation markers. ELISA analysis showed an obvious increase in the concentration of GDNF (a niche factor secreted by Sertoli cells) in the medium when treated with melatonin. Meanwhile, the phosphorylation level of AKT, a downstream of GDNF-GFRa1-RET pathway was activated. In conclusion, melatonin promotes goat SSC proliferation by stimulating GDNF production in Sertoli cells.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

Niu

et al. 2016

Oncotarget

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“…This result differs from those found in other species. The melatonin receptor MT 1 has been previously detected in isolated Leydig cells from Syrian hamster testes [17], whereas both MT 1 and MT 2 receptors have been evidenced in isolated bovine Sertoli cells [52]. However, these differences could be due to the dissimilar identification methods for melatonin receptors.…”

Section: Discussionmentioning

confidence: 99%

Melatonin MT1 and MT2 Receptors in the Ram Reproductive Tract

González-Arto

Aguilar

Gaspar-Torrubia

et al. 2017

IJMS

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Some melatonin functions in mammals are exerted through MT1 and MT2 receptors. However, there are no reports of their presence in the reproductive tract of the ram, a seasonal species. Thus, we have investigated their existence in the ram testis, epididymis, accessory glands and ductus deferens. Real-time polymerase chain reaction (qPCR) revealed higher levels of m-RNA for both receptors in the testis, ampulla, seminal vesicles, and vas deferens, than in the other organs of the reproductive tract (p < 0.05). Western blot analyses showed protein bands compatible with the MT1 in the testis and cauda epididymis, and for the MT2 in the cauda epididymis and deferent duct. Immunohistochemistry analyses revealed the presence of MT1 receptors in spermatogonias, spermatocytes, and spermatids, and MT2 receptors in the newly-formed spermatozoa in the testis, whereas both receptors were located in the epithelial cells of the ampulla, seminal vesicles, and ductus deferens. Indirect immunofluorescence showed significant differences in the immunolocation of both receptors in spermatozoa during their transit in the epididymis. In conclusion, it was demonstrated that melatonin receptors are present in the ram reproductive tract. These results open the way for new studies on the molecular mechanism of melatonin and the biological significance of its receptors.

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“…Melatonin action must be mediated by specific melatonin receptors; therefore, mutations in the MTNR1A gene could affect BMTs and MQTs. The polymorphism of MTNR1A gene had been extensive studies in Sarda goat, Rasa Aragonesa sheep, pig, Sika deer and Mediterranean Italian buffaloes, and association studies revealed a significant relationship with reproductive activities (Chu et al, 2007;Martínez-Royo et al, 2012;Yang et al, 2014aYang et al, , 2014b. However, studies of MTNR1A with BMTs and MQTs are relatively rare.…”

Section: Discussionmentioning

confidence: 99%

“…In mammals, MTNR1A and MTNR1B have been cloned in many species and it has been found that MTNR1A has a unique capacity for high-affinity binding to melatonin and that it mediates numerous physiological functions (Reppert et al, 1996;Lai et al, 2013;Yang et al, 2014aYang et al, , 2014b. Based on this background, it is rational to hypothesize that the MTNR1A gene may be correlated with body measurement traits (BMTs) and meat quality traits (MQTs).…”

Section: Introductionmentioning

confidence: 99%

Association study and expression analysis of MTNR1A as a candidate gene for body measurement and meat quality traits in Qinchuan cattle

Wang

Zan

2015

Gene

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Melatonin regulates the development and function of bovine Sertoli cells via its receptors MT1 and MT2

Cited by 67 publications

References 40 publications

Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

Melatonin MT1 and MT2 Receptors in the Ram Reproductive Tract

Association study and expression analysis of MTNR1A as a candidate gene for body measurement and meat quality traits in Qinchuan cattle

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