Association study and expression analysis of MTNR1A as a candidate gene for body measurement and meat quality traits in Qinchuan cattle

Wang, Yaning; Fu, Changzhen; Zan, L.

doi:10.1016/j.gene.2015.06.012

Cited by 12 publications

(9 citation statements)

References 42 publications

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“…The same region was found to be a QTL, having an effect on abomasum displacement in German Holstein cattle (Mömke et al, 2013). MNTR1A (melatonin receptor 1A) was previously found associated with intramuscular fat and subcutaneous fat (Yang et al, 2015) in Qinchuan beef cattle, and it was also found to be a candidate gene of serum LDL in our study. In summary, GWAS was conducted using two statistical models on 23 serum biochemical traits in a Chinese Holstein cattle population.…”

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confidence: 76%

Identification of Whole-Genome Significant Single Nucleotide Polymorphisms in Candidate Genes Associated With Serum Biochemical Traits in Chinese Holstein Cattle

et al. 2020

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A genome-wide association study (GWAS) was conducted on 23 serum biochemical traits in Chinese Holstein cattle. The experimental population consisted of 399 cattle, each genotyped by a commercial bovine 50K SNP chip, which had 49,663 SNPs. After data cleaning, 41,092 SNPs from 361 Holstein cattle were retained for GWAS. The phenotypes were measured values of serum measurements of these animals that were taken at 11 days after parturition. Two statistical models, a fixed-effect linear regression model (FLM) and a mixed-effect linear model (MLM), were used to estimate the association effects of SNPs. Genome-wide significant and suggestive thresholds were set up to be 1.22E−06 and 2.43E−06, respectively. In the Chinese Holstein population, FLM identified 81 genome-wide significant (0.05/41,092 = 1.22E−06) SNPs associated with 11 serum traits. Among these SNPs, five SNPs (BovineHD0100005950, ARS-BFGL-NGS-115158, BovineHD1500021175, BovineHD0800028900, and BTB-00442438) were also identified by the MLM to have genome-wide suggestive effects on CHE, DBIL, and LDL. Both statistical models pinpointed two SNPs that had significant effects on the Holstein population. The SNP BovineHD0800028900 (located near the gene LOC101903458 on chromosome 8) was identified to be significantly associated with serum high-and low-density lipoprotein (HDL and LDL), whereas BovineHD1500021175 (located in 73.4Mb on chromosome 15) was an SNP significantly associated with total bilirubin and direct bilirubin (TBIL and DBIL). Further analyses are needed to identify the causal mutations affecting serum traits and to investigate the correlation of effects for loci associated with fatty liver disease in dairy cattle.

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confidence: 76%

Identification of Whole-Genome Significant Single Nucleotide Polymorphisms in Candidate Genes Associated With Serum Biochemical Traits in Chinese Holstein Cattle

et al. 2020

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“…In mammals, melatonin mediates many physiological effects by activating MT1 and MT2. Recent studies have found coexpression of MT1 and MT2 in mammalian tissues such as testis, ovary, and adipose tissue . Additionally, some studies have shown that MT1 is expressed in the suprachiasmatic nucleus of humans and mouse, while MT2 is expressed only in the suprachiasmatic nucleus of mouse; MT1 is expressed in the pars tuberalis of mouse, but not humans, and only MT2 expression was detected in human and pig coronary arteries .…”

Section: Discussionmentioning

confidence: 68%

Melatonin suppresses milk fat synthesis by inhibiting the mTOR signaling pathway via the MT1 receptor in bovine mammary epithelial cells

Wang

Guo

et al. 2019

Journal of Pineal Research

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Milk fat content is an important criterion for assessing milk quality and is one of the main target traits of dairy cattle breeding. Recent studies have shown the importance of melatonin in regulating lipid metabolism, but the potential effects of melatonin on milk fat synthesis in bovine mammary epithelial cells (BMECs) remain unclear. Here, we showed that melatonin supplementation at 10 μmol/L significantly downregulated the mRNA expression of lipid metabolism–related genes and resulted in lower lipid droplet formation and triglyceride accumulation. Moreover, melatonin significantly upregulated melatonin receptor subtype melatonin receptor 1a (MT1) gene expression, and the negative effects of melatonin on milk fat synthesis were reversed by treatment with the nonselective MT1/melatonin receptor subtype melatonin receptor 1b (MT2) antagonist. However, a selective MT2 antagonist did not modify the negative effects of melatonin on milk fat synthesis. In addition, KEGG analysis revealed that melatonin inhibition of milk fat synthesis may occur via the mTOR signaling pathway. Further analysis revealed that melatonin significantly suppressed the activation of the mTOR pathway by restricting the phosphorylation of mTOR, 4E‐BP1, and p70S6K, and the inhibition of melatonin on milk fat synthesis was reversed by mTOR activator MHY1485 in BMECs. Furthermore, in vivo experiments in Holstein dairy cows showed that exogenous melatonin significantly decreased milk fat concentration. Our data from in vitro and in vivo studies revealed that melatonin suppresses milk fat synthesis by inhibiting the mTOR signaling pathway via the MT1 receptor in BMECs. These findings lay a foundation to identify a new potential means for melatonin to modulate the fat content of raw milk in Holstein dairy cows.

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“…Genomic DNA was then diluted to 50 ng/μL for polymerase chain reaction (PCR) analysis. We also collected phenotypic data following the methods of Wang et al [ 10 ] and Yang et al [ 11 ]. Traits measured in this study were BL, WH, HH, HG, abdominal girth (AGR), hip width (HW), and pin bone width (PBW).…”

Section: Methodsmentioning

confidence: 99%

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

et al. 2022

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Objective: The growth differentiation factor 8 (GDF8) gene plays a key role in bone formation, resorption, and skeletal muscle development in mammals. Here, we studied the genetic variants of GDF8 and their contribution to body conformation traits in Chinese Dabieshan cattle.Methods: Single nucleotide polymorphisms were identified in the bovine GDF8 gene by DNA sequencing. Phylogenetic analysis, motif analysis, and genetic diversity analysis were conducted using bioinformatics software. Association analysis between five SNPs, haplotype combinations, and body conformation traits was conducted in 380 individuals.Results: GDF8 was highly conserved in seven species, and the GDF8 sequence of cattle was most similar to the sequences of sheep and goat based on the phylogenetic analysis. The motif analysis showed that there were 12 significant motifs in GDF8. Genetic diversity analysis indicated that the polymorphism information content of the five studied SNPs was within 0.25 to 0.5. Haplotype analysis revealed that a total of 12 different haplotypes and those with a frequency of < 0.05 were excluded. Linkage disequilibrium analysis showed that there was a strong linkage (r 2 > 0.330) between the following SNPs: g.5070C > A, g.5076T > C, and g.5148A > C. Association analysis indicated that these five SNPs were associated with some of the body conformation traits (p < 0.05), and the animals with haplotype combination H1H1 (-GGGGCCTTAA-) had greater wither height, hip height, heart girth, abdominal girth, and pin bone width than the other animals (p < 0.05) in Dabieshan cattle. Conclusion:Overall, our results indicate that the genetic variants of GDF8 affected the body conformation traits of Chinese Dabieshan cattle, and the GDF8 gene could make a strong candidate gene in Dabieshan cattle breeding programs.

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Association study and expression analysis of MTNR1A as a candidate gene for body measurement and meat quality traits in Qinchuan cattle

Cited by 12 publications

References 42 publications

Identification of Whole-Genome Significant Single Nucleotide Polymorphisms in Candidate Genes Associated With Serum Biochemical Traits in Chinese Holstein Cattle

Identification of Whole-Genome Significant Single Nucleotide Polymorphisms in Candidate Genes Associated With Serum Biochemical Traits in Chinese Holstein Cattle

Melatonin suppresses milk fat synthesis by inhibiting the mTOR signaling pathway via the MT1 receptor in bovine mammary epithelial cells

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

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