Melatonin suppresses milk fat synthesis by inhibiting the <scp>mTOR</scp> signaling pathway via the <scp>MT</scp>1 receptor in bovine mammary epithelial cells

Wang, Yujuan; Guo, Wenli; Xu, Haichao; Tang, Kai-Fu; Liu, Zan; Yang, Wenguang

doi:10.1111/jpi.12593

Cited by 43 publications

(46 citation statements)

References 63 publications

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“…A classic mTOR activator named MHY1485 was applied to validate the internal route of CRYAB commanding M2 polarization in vitro 24,25 . In CRYAB low-expression groups, M2 markers, Akt1/mTOR pathway and phosphorylation of pivotal downriver transcription factors, were declined ( Fig.…”

Section: Trigger Of Akt1/mtor Was Engaged In Cryab-inducing M2 Polarimentioning

confidence: 99%

Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB

et al. 2020

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Hepatic ischemia/reperfusion injury (IRI) is an unavoidable course in liver transplantation, during which the immune response of inflammation plays a leading part. MicroRNA-450b-5p (miR-450b-5p), which has been reported to participate in several inflammatory diseases, was investigated in this study. The purpose of this study is to identify the potential function of miR-450b-5p toward remission of hepatic IRI and elucidate the specific mechanism. Herein we found that expression of miR-450b-5p, interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), and IL-6 was stimulated in hepatic IRI. Inhibition of miR-450b-5p could remarkably alleviate mouse hepatic IRI and improve liver function measured by hematoxylin-eosin (HE) staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), and enzyme-linked immunosorbent assay (ELISA). We further assessed protein expression undergoing Western blot and immunofluorescence, and discovered that miR-450b-5p suppressed alpha B-crystallin (CRYAB), thus restraining the inhibitory κB kinase (IKK) β-mediated canonical nuclear factor-κB (NF-κB) signaling, instead of the noncanonical path guided by IKKα in hepatic IRI. In addition, we demonstrated CRYAB as an activator of M2 polarization through protein kinase B (Akt) 1/mammalian target of rapamycin (mTOR), thus resulting in relief of liver IRI. Combination treatment containing both paths revealed a better antidamage efficacy than adjusting either path alone, suggesting that the joint therapy might be a promising solution in hepatic IRI.

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Section: Trigger Of Akt1/mtor Was Engaged In Cryab-inducing M2 Polarimentioning

confidence: 99%

Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB

et al. 2020

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“…In this study, we observed that melatonin decreased milk fat content in different seasons, ages and lactation periods. Molecular mechanism study has identi ed that melatonin inhibits the mTOR signaling pathway through MT1 receptors in bovine mammary epithelial cells and via this pathway melatonin suppresses the synthesis of milk fat [50]. This observation provides a new basis for applying melatonin to regulate milk fat metabolism.…”

Section: Discussionmentioning

confidence: 93%

Effects of melatonin on Dairy Herd Improvement (DHI) of Holstein cow with mastitis

Yao

Wang

et al. 2020

Preprint

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Background: Mastitis is a common disease in cows breeding. The milk quality will be significantly reduced with increased milk somatic cells which often occur in cows with mastitis. In the current study, we reported that melatonin significantly reduced the milk somatic cell count in Holstein cow with mastitis. In addition, the nutritional value of the milk also significantly improved by melatonin treatment. Results: The results showed that melatonin significantly suppressed the milk somatic cell score under any of the tested conditions. A long term of melatonin application (one year) to the cows with mastitis suppressed the somatic cell score by roughly 63% which was more efficiency than that of some antibiotic treatments. Melatonin treatment also improved the milk nutritional value by reducing its fat but increasing its lactose and protein contents. Conclusions: Melatonin application significantly improved DHI. The beneficial effects of melatonin on DHI are likely attributed to the antioxidant and anti-inflammatory activities of melatonin.

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“…BMECs were collected using 0.25% trypsin (Solarbio, Beijing, China) and lysed in RIPA buffer (Solarbio, Beijing, China) containing 1% PMSF (Pierce, Rockford, IL, USA). Western blot was carried out as described previously [5]. Primary antibodies against β-actin (mAbcam 8226) (1:2000, Abcam), Smad3 (C679H) (1:1000, Cell Signaling Technology), and HRP goat Anti-Rabbit IgG secondary antibody (ab97051) (1:2000, Abcam) were used.…”

Section: Western Blot Assaymentioning

confidence: 99%

“…Cow milk is a natural food with high nutritional benefits and is therefore considered as basic food in many diets. Milk fat is the main determinant of its nutritional value, and as such it is usually applied to indicate milk quality [1,2], and therefore its synthesis process has been widely studied [3][4][5]. Milk fat synthesis is influenced by several factors, such as heredity, hormone, physiology, environment, and nutrition [6][7][8], but the understanding of the regulation mechanism of milk fat synthesis is still minimal.…”

Section: Introductionmentioning

confidence: 99%

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2020

Animals

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Milk fat is the main nutritional component of milk and is also an important indicator for evaluating milk quality. Substantial evidence has implicated miRNAs in the synthesis of milk fat. miR-143 is one of the miRNAs closely related to lipid metabolism. Herein, miR-143 upregulation remarkably promoted the production of lipid droplets and increased the level of intracellular triglyceride (TAG). Meanwhile, miR-143 suppression overtly repressed TAG synthesis and lipid droplet accumulation in bovine mammary epithelial cells (BMECs). At the same time, miR-143 significantly upregulated the genes associated with lipid synthesis, including PPARγ, SCD1, CEBPβ, and SREBP1. To examine the regulatory mechanism of miR-143 in milk fat synthesis, Smad3 was predicted as a new potential miR-143 target gene by TargetScan. Further studies found that miR-143 expression was inversely related to the levels of Smad3 mRNA and protein. Furthermore, luciferase reporter assays confirmed Smad3 to be a miR-143 direct target. Moreover, Smad3 gene silencing significantly increased intracellular TAG level in BMECs. These findings revealed that miR-143 promotes the TAG synthesis in BMECs via increasing the lipid synthesis related gens expression by targeting Smad3. The results of this study can be exploited in devising novel approaches for improving the nutritional value of milk in dairy cows.

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Melatonin suppresses milk fat synthesis by inhibiting the mTOR signaling pathway via the MT1 receptor in bovine mammary epithelial cells

Cited by 43 publications

References 63 publications

Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB

Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB

Effects of melatonin on Dairy Herd Improvement (DHI) of Holstein cow with mastitis

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

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