Melatonin protects mast cells against cytotoxicity mediated by chemical stimuli PMACI: Possible clinical use

Maldonado, Mónica; García-Moreno, Héctor; Calvo, Juan R.

doi:10.1016/j.jneuroim.2013.06.013

Cited by 12 publications

(15 citation statements)

References 43 publications

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“…PMACI chemical stimulation triggered a state of hyper‐stimulation in the mast cells that led to cellular apoptosis [Maldonado et al, ]. Cell viability was determined by using the MTT assay.…”

Section: Resultsmentioning

confidence: 99%

“…Supporting this idea, the luzindole, a non‐selective melatonin receptor antagonist, blocked their protection by 1 mM melatonin, but not completely. This may well be due to the lingering presence of MELx in mast cells, which renders any cytoprotection, via melatonin receptor independent [Reiter et al, ; Maldonado et al, ].…”

Section: Discussionmentioning

confidence: 99%

“…Mast cells were subjected to PMACI stimulation, with and without pre‐treatment of MELx. PMACI is formed by the union of PMA (phorbol‐12‐myristate 13‐acetate) used at doses of 50 × 10 −8 M and CI (calcium ionophore A23187) used at doses of 5 × 10 −7 M, for 2 and 12 h. Previous pilot experiments of this study helped towards the selection of the times and concentrations of the combined PMA and CI for optimal cell stimulation in subsequent experiments [Maldonado et al, ]. Cell viability was determined by using 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT assay; Sigma–Aldrich).…”

Section: Methodsmentioning

confidence: 99%

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Possible Involvement of the Inhibition of NF‐κB Factor in Anti‐Inflammatory Actions That Melatonin Exerts on Mast Cells

Maldonado

García-Moreno

González-Yanes

et al. 2016

J of Cellular Biochemistry

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Melatonin is a molecule endogenously produced in a wide variety of immune cells, including mast cells (RBL-2H3). It exhibits immunomodulatory, anti-inflammatory and anti-apoptotic properties. The physiologic mechanisms underlying these activities of melatonin have not been clarified in mast cells. This work is designed to determine the anti-inflammatory effect and mechanism of action of melatonin on activated mast cells. RBL-2H3 were pre-treated with exogenous melatonin (MELx) at physiological (100nM) and pharmacological (1 mM) doses for 30 min, washed and activated with PMACI (phorbol 12-myristate 13-acetate plus calcium ionophore A23187) for 2 h and 12 h. The data shows that pre-treatment of MELx in stimulated mast cells, significantly reduced the levels of endogenous melatonin production (MELn), TNF-α and IL-6. These effects are directly related with the MELx concentration used. MELx also inhibited IKK/NF-κB signal transduction pathway in stimulated mast cells. These results indicate a molecular basis for the ability of melatonin to prevent inflammation and for the treatment of allergic inflammatory diseases through the down-regulation of mast cell activation. J. Cell. Biochem. 117: 1926-1933, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Possible Involvement of the Inhibition of NF‐κB Factor in Anti‐Inflammatory Actions That Melatonin Exerts on Mast Cells

Maldonado

García-Moreno

González-Yanes

et al. 2016

J of Cellular Biochemistry

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“…Nas células mononucleares do sistema imunológico, o aumento da atividade fagocitária induzido pela melatonina é mediado pelo receptor MT2 (Pires-Lapa et al, 2013), enquanto que a indução da expressão de IL-2 ocorre via ativação de receptores MT1 (Carrillo-Vico et al, 2003). Já o efeito anti-apoptótico da melatonina sobre as células do sistema imunológico pode ser tanto dependente (Radogna et al, 2007;Maldonado et al, 2013) quanto independente (Hardeland, 2009;Espino et al, 2010) da ativação de receptores de melatonina.…”

Section: Mecanismos De Ação Da Melatoninaunclassified

Sistema melatonérgico como alvo do peptídeo β-amiloide

Cecon¹,

Markus²

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Aβ peptídeo beta amiloide APP proteína precursora amiloide AA-NAT enzima arilalquilamina N-acetiltransferase ACh acetilcolina ASMT enzima acetilserotonina metiltransferase AD doença de Alzheimer AFMK N1-acetil-N2-formil-5-metoxiquinuramina AMK N1-acetil-5-metoxiquinuramina AMPA receptor de ácido α-amino-3-hidroxi-5-metil-4-isoxazolepropiônico AMPc monofosfato cíclico de adenosina AP-1 proteína ativadora 1 AT2 receptor de angiotensina II ATP adenosina trifosfato BCG Bacilo Calmette-Guerin BRET transferência de energia bioluminescente por ressonância COX enzima ciclooxigenase CREB proteína de ligação ao elemento responsivo ao AMPc

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“…Em linfócitos humano, a MEL desempenha papel crucial na modulação do sistema de receptores IL-2/IL2R, sendo essa interleucina fundamental na maturação de linfócitos (Carrilo-Vico et al, 2004). Nos mastócitos, a MEL tem efeito protetor contra citotoxicidade (Maldonado et al, 2013) e no tecido endotelial a melatonina atua na redução do rolamento e adesão de neutrófilos, tendo assim, um possível efeito regulatório sobre a montagem de respostas inflamatórias (Tamura et al, 2010).…”

Section: Introdução 11 Melatoninaunclassified

Efeitos da corticosterona sobre a produção de melatonina em macrófagos de linhagem RAW 264.7

Silva¹

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Melatonin protects mast cells against cytotoxicity mediated by chemical stimuli PMACI: Possible clinical use

Cited by 12 publications

References 43 publications

Possible Involvement of the Inhibition of NF‐κB Factor in Anti‐Inflammatory Actions That Melatonin Exerts on Mast Cells

Possible Involvement of the Inhibition of NF‐κB Factor in Anti‐Inflammatory Actions That Melatonin Exerts on Mast Cells

Sistema melatonérgico como alvo do peptídeo β-amiloide

Efeitos da corticosterona sobre a produção de melatonina em macrófagos de linhagem RAW 264.7

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