Melatonin may play a role in modulation of bax and bcl-2 expression levels to protect rat peripheral blood lymphocytes from gamma irradiation-induced apoptosis

Mohseni, Mehran; Mihandoost, Ehsan; Shirazi, Alireza; Sepehrizadeh, Zargham; Bazzaz, Javad Tavakkoly; Ghazi‐Khansari, Mahmoud

doi:10.1016/j.mrfmmm.2012.08.006

Cited by 69 publications

(47 citation statements)

References 48 publications

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“…In addition, Akt can protect cellular apoptosis by regulating a proapoptotic protein such as Bax [121–124]. Several studies demonstrated that melatonin may regulate the Bax expression and may be involved in the apoptosis signaling [125, 126]. In the present study, our results showed that melatonin may regulate the Bax expression through regulating Akt activation.…”

Section: Discussionsupporting

confidence: 69%

The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury

Song

Kang

Lee

et al. 2014

Oxidative Medicine and Cellular Longevity

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Melatonin has a cellular protective effect in cerebrovascular and neurodegenerative diseases. Protection of brain endothelial cells against hypoxia and oxidative stress is important for treatment of central nervous system (CNS) diseases, since brain endothelial cells constitute the blood brain barrier (BBB). In the present study, we investigated the protective effect of melatonin against oxygen-glucose deprivation, followed by reperfusion- (OGD/R-) induced injury, in bEnd.3 cells. The effect of melatonin was examined by western blot analysis, cell viability assays, measurement of intracellular reactive oxygen species (ROS), and immunocytochemistry (ICC). Our results showed that treatment with melatonin prevents cell death and degradation of tight junction protein in the setting of OGD/R-induced injury. In response to OGD/R injury of bEnd.3 cells, melatonin activates Akt, which promotes cell survival, and attenuates phosphorylation of JNK, which triggers apoptosis. Thus, melatonin protects bEnd.3 cells against OGD/R-induced injury.

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Section: Discussionsupporting

confidence: 69%

The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury

Song

Kang

Lee

et al. 2014

Oxidative Medicine and Cellular Longevity

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show abstract

“…These changes jeopardize the quality of in vitro cultivated microinjected pronuclear embryos and cause substantially low pregnancy and birth rates when these embryos are implanted into recipients. As mentioned previously, melatonin is a potent free radical scavenger and antioxidant, and the beneficial effects of melatonin on embryo development have been attributed to its effects on increasing intracellular glutathione, reducing ROS production, upregulating an anti-apoptotic gene ( Bcl-2 ), and downregulating pro-apoptotic genes ( Bax and caspase-3 ) [14,15,32]. Therefore, the supplementation of melatonin with a 10 −9 M concentration lowered the apoptosis of blastocysts and improved both the embryo quality and the efficiency of embryo implantation [16].…”

Section: Discussionmentioning

confidence: 99%

Melatonin Promotes the In Vitro Development of Microinjected Pronuclear Mouse Embryos via Its Anti-Oxidative and Anti-Apoptotic Effects

Tian

Feng

Zhang

et al. 2017

IJMS

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CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) combined with pronuclear microinjection has become the most effective method for producing transgenic animals. However, the relatively low embryo developmental rate limits its application. In the current study, it was observed that 10−7 M melatonin is considered an optimum concentration and significantly promoted the in vitro development of murine microinjected pronuclear embryos, as indicated by the increased blastocyst rate, hatching blastocyst rate and blastocyst cell number. When these blastocysts were implanted into recipient mice, the pregnancy rate and birth rate were significantly higher than those of the microinjected control, respectively. Mechanistic studies revealed that melatonin treatment reduced reactive oxygen species (ROS) production and cellular apoptosis during in vitro embryo development and improved the quality of the blastocysts. The implantation of quality-improved blastocysts led to elevated pregnancy and birth rates. In conclusion, the results revealed that the anti-oxidative and anti-apoptotic activities of melatonin improved the quality of microinjected pronuclear embryos and subsequently increased both the efficiency of embryo implantation and the birth rate of the pups. Therefore, the melatonin supplementation may provide a novel alternative method for generating large numbers of transgenic mice and this method can probably be used in human-assisted reproduction and genome editing.

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“…With MT1 and MT2 receptors, melatonin was found to inhibit the production of forskolin-stimulated cyclic AMP (cAMP), cyclic GMP (cGMP) and diacylglycerol (DAG), leading to the improved immunity [183,184]. Besides, the specific nuclear melatonin receptors have been identified in Jurkat cells [185], lymphocytes [186], thymus [175] and spleen [187], which belong to the RZR/ROR subfamily of nuclear receptors.…”

Section: Bioactivities Of Melatoninmentioning

confidence: 99%

Dietary Sources and Bioactivities of Melatonin

et al. 2017

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Insomnia is a serious worldwide health threat, affecting nearly one third of the general population. Melatonin has been reported to improve sleep efficiency and it was found that eating melatonin-rich foods could assist sleep. During the last decades, melatonin has been widely identified and qualified in various foods from fungi to animals and plants. Eggs and fish are higher melatonin-containing food groups in animal foods, whereas in plant foods, nuts are with the highest content of melatonin. Some kinds of mushrooms, cereals and germinated legumes or seeds are also good dietary sources of melatonin. It has been proved that the melatonin concentration in human serum could significantly increase after the consumption of melatonin containing food. Furthermore, studies show that melatonin exhibits many bioactivities, such as antioxidant activity, anti-inflammatory characteristics, boosting immunity, anticancer activity, cardiovascular protection, anti-diabetic, anti-obese, neuroprotective and anti-aging activity. This review summaries the dietary sources and bioactivities of melatonin, with special attention paid to the mechanisms of action.

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Melatonin may play a role in modulation of bax and bcl-2 expression levels to protect rat peripheral blood lymphocytes from gamma irradiation-induced apoptosis

Cited by 69 publications

References 48 publications

The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury

The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury

Melatonin Promotes the In Vitro Development of Microinjected Pronuclear Mouse Embryos via Its Anti-Oxidative and Anti-Apoptotic Effects

Dietary Sources and Bioactivities of Melatonin

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