Melatonin maintains the storage quality of fresh-cut Chinese water chestnuts by regulating phenolic and reactive oxygen species metabolism

Xu, Yuhan; Yu, Jingquan; Chen, Jinhui; Gong, Jiabao; Li, Peng; Yi, Yang; Ai, Youwei; Hou, Wenfu; Wang, Hongxun; Min, Ting

doi:10.1093/fqsafe/fyac002

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…AsA is a vitamin substance widely distributed in plant tissues. It participates in the redox effect in the electron transport system in plants and is an important reducing agent in non-enzymatic antioxidant system of plants ( Xu et al., 2022 ). The AsA content in fresh-peeled CWCs generally showed a downward trend ( Figure 8A ).…”

Section: Resultsmentioning

confidence: 99%

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Effect and mechanism of eugenol on storage quality of fresh-peeled Chinese water chestnuts

Chen

Xu²,

Yang³

et al. 2022

Front. Plant Sci.

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The study aimed to investigate the effect and mechanism of eugenol treatment on fresh-peeled Chinese water chestnuts (CWCs). The results found that eugenol treatment maintained the appearance of fresh-peeled CWCs, accompanied by higher L* value, total solids and O2 contents, as well as lower browning degree, weight loss rate, CO2 content, a* and b* values. In addition, eugenol treatment significantly reduced the activities of peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase, as well as the total content of soluble quinone in fresh-peeled CWCs. Meanwhile, fresh-peeled CWCs treated with eugenol showed markedly lower content of total flavonoids, which may be related to yellowing. Furthermore, eugenol treatment suppressed the rates of O2·- and OH·- production as well as the contents of H2O2 and malondialdehyde in fresh-peeled CWCs. During the storage, eugenol treatment not only increased the activities of catalase, superoxide dismutase, ascorbate peroxidase and glutathione reductase as well as the DPPH free radical scavenging rate, but also increased the total phenolics, ascorbic acid and glutathione contents. In summary, eugenol treatment delayed the surface discoloration of fresh-peeled CWCs by improving the antioxidant capacity, inhibiting the phenolic compound metabolism and scavenging ROS, thus effectively maintaining the quality of fresh-peeled CWCs while extending their shelf life.

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Section: Resultsmentioning

confidence: 99%

“…APX and GR are antioxidant enzymes that work together with antioxidant substances to remove ROS to protect the integrity of the cell membrane ( Xu et al., 2022 ). The APX activity of eugenol treatment group was higher than the other two groups.…”

Section: Resultsmentioning

confidence: 99%

Effect and mechanism of eugenol on storage quality of fresh-peeled Chinese water chestnuts

Chen

Xu²,

Yang³

et al. 2022

Front. Plant Sci.

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“…Melatonin has improved the antioxidant capacity of fresh‐cut broccoli significantly to maintain its quality 20 . It also delayed surface discoloration of fresh‐cut Chinese water chestnuts by reducing total flavonoid content 21 …”

Section: Introductionmentioning

confidence: 99%

“…20 It also delayed surface discoloration of fresh-cut Chinese water chestnuts by reducing total flavonoid content. 21 Recent studies attributed the discoloration of fruits and vegetables to flavonoid biosynthesis. 22 Flavonoids and phenolics were synthesized by the metabolic intermediates of the phenylpropanoid pathway through enzymes including PAL, cinnamic acid-4hydroxylase (C4H), 4-coumarateCoA ligase (4CL), chalcone isomerase (CHI), chalcone synthase (CHS), and flavonoid 3 0 hydroxylase (F3 0 H).…”

Section: Introductionmentioning

confidence: 99%

Melatonin‐induced myeloblastosis viral oncogene homologs alleviate fresh‐cut lotus root browning during storage by attenuating flavonoid biosynthesis and reactive oxygen species

et al. 2023

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BACKGROUND Lotus roots (Nelumbo nucifera Gaertn.) are rich in nutrients and have ornamental and food value. However, browning has caused huge economic losses and security risks during the storage and harvesting of fresh‐cut lotus. This study investigated the role of melatonin in inhibiting lotus browning, and illustrates its molecular mechanism. RESULTS The application of melatonin effectively retarded the process of lotus browning, enhanced reactive oxygen species (ROS) scavenging enzyme activity, and inhibited the activity of polyphenol oxidase (PPO), and peroxidase (POD). Melatonin reduced flavonoid content, and decreased enzymatic activity in flavonoid biosynthesis. Transcriptome Sequencing (RNA‐seq) was used to screen the genes regulated by exogenous melatonin when defending against fresh‐cut lotus browning. Gene co‐expression analysis (GCN) indicated that the transcription factors MYB5, MYB6, and MYB308, activated by melatonin, were negatively related to the expression of PPO and the genes related to flavonoid and phenylpropanoid biosynthesis. These myeloblastosis viral oncogene homologs (MYBs) were positively related to the expression of genes encoding the enzymes in glutathione metabolism. CONCLUSION Melatonin retarded lotus browning by transcriptional suppression of key genes associated with flavonoid and phenylpropanoid biosynthesis through the stimulation of MYB5, MYB6, and MYB308. © 2023 Society of Chemical Industry.

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“…In plum fruits, suitable concentration of MT treatment could keep the contents of fruit soluble solids and titratable acid stable and ensure the fruit flavor and quality ( Feng et al, 2020 ). MT could inhibit phenolic compound metabolism and improve antioxidant capacity to delay the surface discoloration of fresh-cut Chinese water chestnuts and prolong the shelf life ( Xu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

The mechanism analysis of exogenous melatonin in limiting pear fruit aroma decrease under low temperature storage

Wei

Jiao

Wang

et al. 2022

PeerJ

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Exogenous melatonin (MT) is widely used in fruit preservation, and can increase the storage time and delay the quality deterioration. Firstly, it was found that 150 μM MT was the optimal concentration to treat ‘Xinli No.7’ under storage at 4 °C for 60 days. MT could significantly improve oxidase activity and inhibit the reduction of physiological indexes, including pulp hardness, weight loss, titratable acid and soluble solid content. MT could also reduce ethylene release and limit the reduction of fruit aroma. The average content of fruit aroma substance increased by 43.53%. A relevant RNA-Seq database was built to further explore the regulation mechanism of MT. A total of 2,761 differentially expressed genes (DEGs) were identified. DEGs were enriched in 64 functional groups and 191 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. DEGs were mainly enriched in alpha-linolenic acid metabolism, fatty acid metabolism and plant hormone signal transduction pathway. The gene pycom09g05270 belonging to long chain acyl-CoA synthetase family and participating in fatty acid metabolism pathway was identified, and its expression level was consistent with fragments per kilobase per million mapped reads (FPKM) values, implying that pycom09g05270 might play a vital role in maintaining quality during the storage process.

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Melatonin maintains the storage quality of fresh-cut Chinese water chestnuts by regulating phenolic and reactive oxygen species metabolism

Cited by 6 publications

References 32 publications

Effect and mechanism of eugenol on storage quality of fresh-peeled Chinese water chestnuts

Effect and mechanism of eugenol on storage quality of fresh-peeled Chinese water chestnuts

Melatonin‐induced myeloblastosis viral oncogene homologs alleviate fresh‐cut lotus root browning during storage by attenuating flavonoid biosynthesis and reactive oxygen species

The mechanism analysis of exogenous melatonin in limiting pear fruit aroma decrease under low temperature storage

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