Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Bulan, Nihal Ömür; Sarikaya-Unal, Guner; Tunalı, Sevim; Pirincci, Pelin Arda; Yanardağ, Refiye

doi:10.3906/biy-1507-53

Cited by 4 publications

(2 citation statements)

References 63 publications

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“…This enzyme activity increases due to oxidative stress. It has been shown that the MPO is enhanced in rats given Al by various researchers [35,36]. Our results demonstrated that in Al combination group toxicity, administration of Mel inhibited neutrophil infiltration and balanced oxidant-antioxidant levels in the brain tissue.…”

Section: Discussionsupporting

confidence: 61%

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

Karabulut-Bulan¹,

Bayrak²,

Sarikaya-Unal³

et al. 2019

jrp

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Aluminum (Al), an ubiquitous element in nature, enters the body primarily through gastrointestinal tract, respiratory system and skin. Being a powerful neurotoxin for human brain, Al was reported to be involved in the etiology of Alzheimer's disease due to its easy access and accumulation in the central nervous system. Melatonin (Mel) is a tryptophan-derived neurohormone in animals and plants, and produced in the pineal gland of all mammalian species. The present study examines the effects of Mel on Al-induced oxidative stress, inflammation, tissue factor production and brain damage in rat brain. Wistar albino rats were divided into four groups. Group I: control animals; Group II: rats injected with 10 mg/kg Mel; Group III: rats injected with 5 mg/kg Al2(SO4)3; and Group IV: rats injected combination of Al and Mel (5 mg/kg Al2(SO4)3 and 10 mg/kg Mel). Animals were injected three times a week for one month. At the end of the month, rats were sacrificed, their brains were removed. It was found that lipid peroxidation, protein carbonyl, advanced oxidation protein products, hydroxyproline levels, tissue factor, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, myeloperoxidase, acetylcholine esterase, alkaline phosphatase, acid phosphatase and glucose-6-phosphate dehydrogenase activities were increased, while paraoxonase, arylesterase, sodium potassium ATPase activities and glutathione levels were decreased in the Al-treated group. Mel treatment reversed these changes by demonstrating significant antioxidant effects. Results indicated that Mel has potential therapeutic value against Al-induced oxidative stress in the rat brain tissue and these effects may be related to its antioxidant activities.

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Section: Discussionsupporting

confidence: 61%

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

Karabulut-Bulan¹,

Bayrak²,

Sarikaya-Unal³

et al. 2019

jrp

Self Cite

View full text Add to dashboard Cite

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“…Some researchers have reported Al toxicity in the small intestine of rats. Bulan et al (2015) showed that chronic exposure of aluminum sulfate induced degenerative tissue changes and oxidative parameters in the small intestine (Bulan et al 2015). In this study, rats were injected intraperitoneally with 5 mg=kg aluminum sulfate [Al 2 ðSO 4 Þ 3 ].…”

Section: Environmental Health Perspectivesmentioning

confidence: 99%

Effects of Aluminum on the Integrity of the Intestinal Epithelium: An in Vitro and in Vivo Study

Jeong

Kwon

Kim

et al. 2020

Environ Health Perspect

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BACKGROUND: Aluminum (Al) is the most abundant and ubiquitous metal in the environment. The main route of human exposure to Al is through food and water intake. Although human exposure to Al is common, the influence of Al on the gastrointestinal tract remains poorly understood. OBJECTIVES: We aimed to further understand the toxic effect of Al and to elucidate the underlying cellular mechanisms in the intestinal barrier. METHODS: The human intestinal epithelial cell line HT-29 and C57BL6 mice were exposed to AlCl 3 at 0-16 mM (1-24 h) and 5-50 mg=kg body weight (13 weeks), respectively. In cell culture experiments, intracellular oxidative stress, inflammatory protein and gene expression, and intestinal epithelial permeability were measured. In animal studies, histological examination, gene expression, and myeloperoxidase (MPO) activity assays were conducted. RESULTS: Cellular oxidative stress level (superoxide production) in AlCl 3-treated cells (4 mM, 3 h) was approximately 38-fold higher than that of the control. Both protein and mRNA expression of tight junction (TJ) components (occludin and claudin-1) in AlCl 3-treated cells (1-4 mM, 24 h) was significantly lower than that of the control. Transepithelial electrical resistance (TEER) decreased up to 67% in AlCl 3-treated cells (2 mM, 24 h) compared with that of the control, which decreased approximately 7%. Al activated extracellular signal-regulated kinase 1/2 and nuclear factor-kappa B (NF-jB), resulting in mRNA expression of matrix metalloproteinase-9, myosin light-chain kinase, and inflammatory cytokines [tumor necrosis factor alpha (TNF-a), interleukin-1b (IL-1b), and IL-6] in HT-29 cells. Moreover, oral administration of AlCl 3 to mice induced pathological alteration, MPO activation, and inflammatory cytokine (TNF-a, IL-1b, and IL-6) production in the colon. CONCLUSION: Al induced epithelial barrier dysfunction and inflammation via generation of oxidative stress, down-regulation of the TJ proteins, and production of inflammatory cytokines in HT-29 cells. In addition, Al induced toxicity in the colon by increasing the levels of inflammatory cytokines and MPO activity and induced histological damage in a mouse model. Our data suggest that Al may be a potential risk factor for human intestinal diseases.

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Melatonin ameliorates sodium valproate-induced hepatotoxicity in rats

et al. 2019

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Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Cited by 4 publications

References 63 publications

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

Effects of Aluminum on the Integrity of the Intestinal Epithelium: An in Vitro and in Vivo Study

Melatonin ameliorates sodium valproate-induced hepatotoxicity in rats

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