Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats

Kahya, Mehmet Cemal; Nazıroğlu, Mustafa; Çiğ, Bilal

doi:10.3109/02699052.2015.1053526

Cited by 35 publications

(22 citation statements)

References 42 publications

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“…Melatonin also showed its antioxidant effect by decreasing malondialdehyde level and increasing glutathione level, superoxide dismutase, catalase, and glutathione‐S‐transferase activities. Baydas et al and Kahya et al found similar results showing that melatonin decreased lipid peroxidation and increased glutathione level in the brain tissue of diabetic rats . In histological evaluation, rats treated with melatonin revealed decreased neuronal degeneration when compared with the diabetic rats.…”

Section: Discussionmentioning

confidence: 64%

“…In this study, melatonin was also added to the insulin treatment showing that melatonin decreased lipid peroxidation and increased glutathione level in the brain tissue of diabetic rats. 81,82 In histological evaluation, rats treated with melatonin revealed decreased neuronal degeneration when compared with the diabetic rats. Main differences between the effects of melatonin alone and insulin alone treatments were observed in glutathione level, glutathione-S-transferase, myeloperoxidase, and TF activity and in histopathological scores.…”

Section: Histological Resultsmentioning

confidence: 99%

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Melatonin improves hyperglycemia induced damages in rat brain

Gurel-Gokmen

Ipekci

Oktay

et al. 2018

Diabetes Metabolism Res

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Section: Discussionmentioning

confidence: 64%

Section: Histological Resultsmentioning

confidence: 99%

Melatonin improves hyperglycemia induced damages in rat brain

Gurel-Gokmen

Ipekci

Oktay

et al. 2018

Diabetes Metabolism Res

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“…CGRP is a pain-transmitting neuropeptide present in the DRG, which is always up-regulated in pain conditions 72. TNF-α and IL-1β are critical Mel-mediated molecules in neuro-inflammation 53, 73, and their expression is associated with neuronal excitability and sensitization 74, 75. Our results indicated that cuff implantation increased the expression of c-fos , CGRP, and the inflammatory cytokines TNF-1α and IL-1β, which indicated pain sensation after cuff implantation.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

Lin

Zhao

et al. 2017

Theranostics

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Melatonin (Mel) and its receptors (MT1 and MT2) have a well-documented efficacy in treating different pain conditions. However, the anti-nociceptive effects of Mel and Mel receptors in neuropathic pain (NP) are poorly understood. To elucidate this process, pain behaviors were measured in a dorsal root ganglia (DRG)-friendly sciatic nerve cuffing model. We detected up-regulation of MT2 expression in the DRGs of cuff-implanted mice and its activation by the agonist 8-M-PDOT (8MP). Also, Mel attenuated the mechanical and thermal allodynia induced by cuff implantation. Immunohistochemical analysis demonstrated the expression of MT2 in the DRG neurons, while MT1 was expressed in the satellite cells. In cultured primary neurons, microarray analysis and gene knockdown experiments demonstrated that MT2 activation by 8MP or Mel suppressed calcium signaling pathways via MAPK1, which were blocked by RAR-related orphan receptor alpha (RORα) activation with a high dose of Mel. Furthermore, expression of nitric oxide synthase 1 (NOS1) was down-regulated upon Mel treatment regardless of MT2 or RORα. Application of Mel or 8MP in cuff-implanted models inhibited the activation of peptidergic neurons and neuro-inflammation in the DRGs by down-regulating c-fos, calcitonin gene-related peptide [CGRP], and tumor necrosis factor-1α [TNF-1α] and interleukin-1β [IL-1β]. Addition of the MT2 antagonist luzindole blocked the effects of 8MP but not those of Mel. In conclusion, only MT2 was expressed in the DRG neurons and up-regulated upon cuff implantation. The analgesic effects of Mel in cuff-implanted mice were closely associated with both MT2-dependent (MAPK-calcium channels) and MT2-independent (NOS1) pathways in the DRG.

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“…Brain samples were obtained and prepared as previously described (Dilek et al 2010;Senol et al 2014;Kahya et al 2015). The removed tissues of brain cortex samples were washed twice with cold physiological saline.…”

Section: Preparation Of Brain Samplesmentioning

confidence: 99%

“…Plasma cytokine [TNF-α, interleukin (IL)-1β, and IL-4] levels were measured using a commercial enzyme-linked immunosorbent assay (ELISA), following the manufacturer's instructions as described in previous studies (Senol et al 2014;Kahya et al 2015) (Multimode microplate reader, Infinite® 200 PRO series, Männedorf Switzerland). They employ the quantitative sandwich techniques.…”

Section: Cytokine Determinations In Plasmamentioning

confidence: 99%

Raloxifene and Tamoxifen Reduce PARP Activity, Cytokine and Oxidative Stress Levels in the Brain and Blood of Ovariectomized Rats

Yazğan

Nazıroğlu

2016

J Mol Neurosci

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It is well known that 17β-estradiol (E2) has an antioxidant role on neurological systems in the brain. Raloxifene (RLX) and tamoxifen (TMX) are selective estrogen receptor modulators. An E2 deficiency stimulates mitochondrial functions for promoting apoptosis and increasing reactive oxygen species (ROS) production. However, RLX and TMX may reduce the mitochondrial ROS production via their antioxidant properties in the brain and erythrocytes of ovariectomized (OVX) rats. We aimed to investigate the effects of E2, RLX, and TMX on oxidative stress, apoptosis, and cytokine production in the brain and erythrocytes of OVX rats.Forty female rats were divided into five groups. The first group was used as a control group. The second group was the OVX group. The third, fourth, and fifth groups were OVX + E2, OVX + TMX, and OVX + RLX groups, respectively. E2, TMX, and RLX were given subcutaneously to the OVX + E2 and OVX + TMX, OVX + RLX groups for 14 days after the ovariectomy respectively.While brain and erythrocyte lipid peroxidation levels were high in the OVX group, they were low in the OVX + E2, OVX + RLX, and OVX + TMX groups. OVX + E2, OVX + RLX, and OVX + TMX treatments increased the lowered glutathione peroxidase activity in erythrocytes and the brain and reduced glutathione and vitamin E concentrations in the brain. β-carotene and vitamin A concentrations in the brain and TNF-α and interleukin (IL)-1β levels in the plasma of the five groups were not significantly changed by the treatments. However, increased plasma IL-4 levels and Western blot results for brain poly (ADP-ribose) polymerase (PARP) in the OVX groups were decreased by E2, TMX, and RLX treatments, although proapoptotic procaspase 3 and 9 activities were increased by the treatments.In conclusion, we observed that E2, RLX, and TMX administrations were beneficial on oxidative stress, inflammation, and PARP levels in the serum and brain of OVX rats by modulating antioxidant systems, DNA damage, and cytokine production.

show abstract

Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats

Abstract: Melatonin and selenium induced protective effects against diabetes-induced brain and erythrocyte oxidative injuries through regulation of the antioxidant level and cytokine production.

Cited by 35 publications

References 42 publications

Melatonin improves hyperglycemia induced damages in rat brain

Melatonin improves hyperglycemia induced damages in rat brain

Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

Raloxifene and Tamoxifen Reduce PARP Activity, Cytokine and Oxidative Stress Levels in the Brain and Blood of Ovariectomized Rats

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