Melanocortin-4 receptor regulation of reproductive function in black rockfish (Sebastes schlegelii)

Zhang, Ying; Wen, Haishen; Li, Yun; Lyu, Likang; Zhang, Zhanxiong; Wang, Xiaojie; Li, Jian-Shuang; Tao, Ya-Xiong; Qi, Xin

doi:10.1016/j.gene.2020.144541

Cited by 13 publications

(10 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we demonstrated that cloned culter mc4r was predicted to encode a protein of 326 amino acids with similar structural characteristics as MC4Rs of other species (Figure 1A and Supplementary Figure 2), including other teleost MC4Rs (27)(28)(29)(30)(31)37). Phylogenetic analysis revealed that caMC4R clustered with teleost MC4Rs (Figure 1B).…”

Section: Discussionmentioning

confidence: 56%

“…In addition to brain, culter mc4r was present more widely in the periphery in the male, especially in the testis (but not in the ovary) ( Figures 3A,B). These data suggest that teleost MC4Rs might play an important role in regulating reproductive function (28,30,37). Several studies have investigated the roles of teleost MC4R in regulating reproductive function (33, 35-37, 54, 55).…”

Section: Discussionmentioning

confidence: 81%

“…MC4R has also been characterized in teleosts (21,(24)(25)(26)(27)(28)(29)(30)(31)(32), and shown to act as a regulator in energy balance, sexual behavior, and reproduction (33)(34)(35)(36)(37). Non-mammalian MRAPs are different from those of mammals.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of Melanocortin-4 Receptor Pharmacology by Two Isoforms of Melanocortin Receptor Accessory Protein 2 in Topmouth Culter (Culter alburnus)

Tao

Huang

et al. 2020

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

Melanocortin-4 receptor (MC4R) plays important roles in regulation of multiple physiological processes, and interaction of MC4R and melanocortin receptor accessory protein 2 (MRAP2) is suggested to play pivotal role in energy balance of vertebrates. Topmouth culter (Culter alburnus) is an economically important freshwater fish in China. Herein we cloned culter mc4r, mrap2a, and mrap2b. Culter mc4r consisted of a 981 bp open reading frame encoding a protein of 326 amino acids. qRT-PCR revealed that mc4r, mrap2a, and mrap2b were primarily expressed in the central nervous system. In the periphery, mc4r and mrap2b were expressed more widely in the male, while mrap2a was expressed more widely in the female. Culter MC4R could bind to four peptide agonists and increase intracellular cAMP production dose dependently. Culter MC4R was constitutively active in both cAMP and ERK1/2 pathways, which was differentially regulated by culter MRAP2a and MRAP2b. Culter MRAP2a significantly increased B max and decreased agonist-stimulated cAMP, while MRAP2b increased cell surface and total expression but did not affect B max and agonist-stimulated cAMP. These results will aid the investigation of the potential physiological processes that MC4R might be involved in topmouth culter.

show abstract

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 81%

See 1 more Smart Citation

Regulation of Melanocortin-4 Receptor Pharmacology by Two Isoforms of Melanocortin Receptor Accessory Protein 2 in Topmouth Culter (Culter alburnus)

Tao

Huang

et al. 2020

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additional studies also demonstrated that asip1 overexpression in a zebrafish transgenic model increased egg production yet reduced size and spawn frequency, once more suggesting that the melanocortin system may modulate the reproductive axis in females [ 5 ]. In fact, a role of Mc4r in the regulation of gonadal development and reproduction [ 21 , 22 , 23 ] of zebrafish and medaka ( Oryzias latipes ) has already been proven, yet involvement of the melanocortin system in the gonad function is poorly understood and no data is available regarding its role in male reproduction. In this study, we take a closer look at the function of the melanocortin system in zebrafish gametogenesis, firstly, by screening the expression of the key components of the system in zebrafish gonads in a single study, followed by examining the in vitro effects of agonists and antagonists on basal and hGC-stimulated steroid secretion in both ovaries and testes.…”

Section: Discussionmentioning

confidence: 99%

Role of the Melanocortin System in Gonadal Steroidogenesis of Zebrafish

Navarro

Crespo

Schulz

et al. 2022

Animals

View full text Add to dashboard Cite

In teleost, as in other vertebrates, stress affects reproduction. A key component of the stress response is the pituitary secretion of the adrenocorticotropic hormone (ACTH), which binds to the melanocortin 2 receptor (MC2R) in the adrenal glands and activates cortisol biosynthesis. In zebrafish, Mc2r was identified in male and female gonads, while ACTH has been shown to have a physiological role in modulating reproductive activity. In this study, the hypothesis that other melanocortins may also affect how the zebrafish gonadal function is explored, specifically steroid biosynthesis, given the presence of members of the melanocortin signaling system in zebrafish gonads. Using cell culture, expression analysis, and cellular localization of gene expression, our new observations demonstrated that melanocortin receptors, accessory proteins, antagonists, and agonists are expressed in both the ovary and testis of zebrafish (n = 4 each sex). Moreover, melanocortin peptides modulate both basal and gonadotropin-stimulated steroid release from zebrafish gonads (n = 15 for males and n = 50 for females). In situ hybridization in ovaries (n = 3) of zebrafish showed mc1r and mc4r in follicular cells and adjacent to cortical alveoli in the ooplasm of previtellogenic and vitellogenic oocytes. In zebrafish testes (n = 3), mc4r and mc1r were detected exclusively in germ cells, specifically in spermatogonia and spermatocytes. Our results suggest that melanocortins are, directly or indirectly, involved in the endocrine control of vitellogenesis in females, through modulation of estradiol synthesis via autocrine or paracrine actions in zebrafish ovaries. Adult zebrafish testes were sensitive to low doses of ACTH, eliciting testosterone production, which indicates a potential role of this peptide as a paracrine regulator of testicular function.

show abstract

“…Therefore, studies on black rockfish immune-related genes can expand our understanding of ncRNA that related to immune response and regulatory mechanisms, and are also helpful to guide the prevention and control of its diseases. Previous studies have investigated several immune-related genes, such as high mobility group box 1, c-type lectin, galectin-8, cathepsin K, chemokine ligand 25, and melanocortin-4, in black rockfish in response to pathogen stimulation (32)(33)(34)(35)(36)(37). However, no systematic report on the interactions of ncRNAs and coding RNAs in S. schlegelii during infection has been performed.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

Cao

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Sebastes schlegelii, an important aquaculture species, has been widely cultured in East Asian countries. With the increase in the cultivation scale, various diseases have become major threats to the industry. Evidence has shown that non-coding RNAs (ncRNAs) have remarkable functions in the interactions between pathogens and their hosts. However, little is known about the mechanisms of circular RNAs (circRNAs) and coding RNAs in the process of preventing pathogen infection in the intestine in teleosts. In this study, we aimed to uncover the global landscape of mRNAs, circRNAs, and microRNAs (miRNAs) in response to Edwardsiella tarda infection at different time points (0, 2, 6, 12, and 24 h) and to construct regulatory networks for exploring the immune regulatory mechanism in the intestine of S. schlegelii. In total, 1,794 mRNAs, 87 circRNAs, and 79 miRNAs were differentially expressed. The differentially expressed RNAs were quantitatively validated using qRT-PCR. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that most of the differentially expressed mRNA genes and the target genes of ncRNAs were related to immune signaling pathways, such as the NF-κB signal pathway, pathogen recognition receptors related to signaling pathways (Toll-like receptors and Nod-like receptors), and the chemokine signaling pathway. Based on these differentially expressed genes, 624 circRNA-miRNA pairs and 2,694 miRNA-mRNA pairs were predicted using the miRanda software. Integrated analyses generated 25 circRNA-miRNA-mRNA interaction networks. In a novel_circ_0004195/novel-530/IκB interaction network, novel_530 was upregulated, while its two targets, novel_circ_0004195 and IκB, were downregulated after E. tarda infection. In addition, two circRNA-miRNA-mRNA networks related to apoptosis (novel_circ_0003210/novel_152/apoptosis-stimulating of p53 protein 1) and interleukin (novel_circ_0001907/novel_127/interleukin-1 receptor type 2) were also identified in our study. We thus speculated that the downstream NF-κB signaling pathway, p53 signaling pathway, and apoptosis pathway might play vital roles in the immune response in the intestine of S. schlegelii. This study revealed a landscape of RNAs in the intestine of S. schlegelii during E. tarda infection and provided clues for further study on the immune mechanisms and signaling networks based on the circRNA-miRNA-mRNA axis in S. schlegelii.

show abstract

Melanocortin-4 receptor regulation of reproductive function in black rockfish (Sebastes schlegelii)

Cited by 13 publications

References 59 publications

Regulation of Melanocortin-4 Receptor Pharmacology by Two Isoforms of Melanocortin Receptor Accessory Protein 2 in Topmouth Culter (Culter alburnus)

Regulation of Melanocortin-4 Receptor Pharmacology by Two Isoforms of Melanocortin Receptor Accessory Protein 2 in Topmouth Culter (Culter alburnus)

Role of the Melanocortin System in Gonadal Steroidogenesis of Zebrafish

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

Contact Info

Product

Resources

About