Melanin Produced by
            <i>Bordetella parapertussis</i>
            Confers a Survival Advantage to the Bacterium during Host Infection

Hiramatsu, Yukihiro; Nishida, Takashi; Nugraha, Dendi Krisna; Sugihara, Fuminori; Horiguchi, Yasuhiko

doi:10.1128/msphere.00819-21

Cited by 12 publications

(10 citation statements)

References 36 publications

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“…Transposon-based integration of the Km r -P tac -GFP fragment into the B. bronchiseptica genome was confirmed by colony PCR using the primers BS333-F and BS334-R. The integration site of Km r -P tac -GFP was determined as previously described ( 44 ). Briefly, genomic DNA (gDNA) of a single mutant was isolated using the DNeasy Blood and Tissue kit (Qiagen) and digested with Sau3AI (TaKaRa).…”

Section: Methodsmentioning

confidence: 99%

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

et al. 2023

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The pathogenic bacterium Bordetella bronchiseptica , which causes respiratory diseases in various mammals, exhibits distinct Bvg + and Bvg − phenotypes. The former represents the virulent phase, in which the bacteria express a set of virulence factors, while the role of the latter in the bacterial life cycle remains to be understood.

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Section: Methodsmentioning

confidence: 99%

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

et al. 2023

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“…The Bvg + phase is generally considered to represent the virulent phenotype, while Bvg − phase represents the avirulent phenotype. The Bvg − -locked mutant of B. parapertussis , which constitutively exhibits the Bvg − phenotype ( 10 , 11 ), did not increase Bdk-induced cAMP production ( Fig. 2 A ).…”

Section: Resultsmentioning

confidence: 91%

DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα _i GTPases and contributes to cough

Hiramatsu,

Nishida,

Ota

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the G i family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis , which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric G i GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx.

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“…A Himar1‐based transposon vector, pMariG, 11 was introduced into B. bronchiseptica RB50 via biparental conjugation through E.coli S17‐1 λpir. After a 6‐h incubation, the bacteria in the mixture were collected and cultured on BG plates supplemented with Gm for 48 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

Ma,

Nugraha,

Hiramatsu

et al. 2023

Microbiology and Immunology

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The Gram‐negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high‐throughput transposon sequencing (Tn‐seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN‐deficient mutant strain was defective in tracheal colonization when co‐inoculated with the wild‐type strain. rpoN encodes σ54, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.

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Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection

Cited by 12 publications

References 36 publications

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα _i GTPases and contributes to cough

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

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Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection

Cited by 12 publications

References 36 publications

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα i GTPases and contributes to cough

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

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DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gα _i GTPases and contributes to cough