MEKC‐LIF method for analysis of amino acids after on‐capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

Abstract

Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo's physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo's surroundings can determine the embryo's physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo's surroundings was developed. AAs were analyzed by means of MEKC-LIF after on-capillary derivatization by naphthalene-2,3-dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology. The resulting derivatives of all the standard AAs were baseline resolved in the BGE comprised of 35 mM sodium tetraborate, 55 mM SDS, 2.7 M urea, 1 mM BIS-TRIS propane, and 23 mM NaOH within 50 min. The method was applied on an analysis of spent culture media used in assisted reproduction to culture embryos after in vitro fertilization.

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

“…There were 12 papers on in‐capillary derivatisation . The derivatisation was performed by adding the reagents to the separation buffer or by zone injections of reagents and sample.…”

“…An advantage of the in‐capillary mode and zone injections is that multiple reagents and injection configuration can be probed easily. For instance, Celá and co‐workers investigated configurations of three and five injected zones for the derivatisation of amino acids using mixing by diffusion or voltage application . Figure shows the injection scheme.…”

A novel in‐capillary assay for dynamically monitoring fast binding between antibody and peptides using CE

“…Consequently, it is imperative to develop suitable strategies for rapid detection of antibody-peptide interactions in-flow. In 1992, such a concept was presented utilizing incapillary, which was further applied to enzymatic reactions, interaction studies, and in-capillary derivatization [37][38][39][40][41][42].…”

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

“…Compared with traditional separation means like ion exchange chromatography and HPLC , CE, including high‐speed capillary electrophoresis (HSCE), has significant advantages, like high efficiency, less sample consumption and short analysis time. These advantages make CE very suitable for analyzing these compounds including AAs and peptides . Fang et al.…”

“…Compared with traditional separation means like ion exchange chromatography [12] and HPLC [13,14], CE, including high-speed capillary electrophoresis (HSCE), has significant advantages, like high efficiency, less sample consumption and short analysis time. These advantages make CE very suitable for analyzing these compounds including AAs and peptides [15][16][17][18][19][20][21][22][23][24][25][26][27]. Fang et al proposed a picoliterscale spontaneous sample injection technique for HSCE and obtained ultra-high column efficiency in a very short separation time [28,29].…”