Mekani̇k Ve Enzi̇mati̇k Yöntem İle İzole Edi̇len Stromal Vasküler Fraksi̇yon’un Yara İyi̇leşmesi̇ Üzeri̇ndeki̇ Etki̇si̇ni̇n in Vi̇tro İncelenmesi̇

Tiryaki, Kemal Tunç

doi:10.26650/iuitfd.2019.0051

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In many other studies, very different numbers were presented, 4,22,26,28 even in several studies using the same product. 27,29,31 More recently, Cohen et al published a comparison of two different commercial mechanical stromal cell isolation products. 32 The first one was the Tulip NanoTransfer kit (Tulip Medical, Inc., San Diego, CA), which consists of 2.4,1.4 and 1.2 mm connectors and 400 and 600 μ two-layered filters.…”

Section: Discussionmentioning

confidence: 99%

Indication-based protocols with different solutions for mechanical stromal-cell transfer

Çöpçü¹

2022

Scars, Burns & Healing

View full text Add to dashboard Cite

Background Regenerative medicine is the fastest developing branch of plastic surgery in recent times. Adipose tissue is one of the largest and most important sources in the body for stromal cells. Although mechanical isolation methods are both very popular and have many advantages, they still have no accepted protocols. Objective We developed new protocols called indication-based protocols (IPs) for standardization and new techniques called mechanical stromal-cell transfer (MEST) by using ultra-sharp blades and dilution of adipose tissue with different solutions (saline, Ringer and 5% Dextrose) Methods & material: In order to obtain the desired physical structure (liquid, gel, solid) and the desired volume, four different types of IPs have been defined. Adipose tissue was prediluted with different solutions using 10 or 20 cc injectors in IPs 1 and 2, while condensed adipose tissue was used directly in IPs 3 and 4. Results In MEST, stromal cells were obtained from 100 mL of condensed fat using different IPs with 92% mean viability and cell counts of 26.80–91.90 × 106. Stromal cells can be obtained in the desired form and number of cells by using four different IPs. Conclusion Isolation of stromal cells by cutting fat with sharp blades will prevent the death of fat tissue and stromal cells and will allow high viability and cell count with our new technique. Predilution with different solutions: Diluting the condensed adipose tissue with the desired solutions (saline, Ringer or 5% Dextrose) before the adinizing process will provide even more stromal cells. Lay Summary Obtaining regenerative stromal cells from adipose tissue can be done by two methods: Enzymatic and mechanical. Mechanical methods have many advantages. Although mechanical stromal cell extraction from adipose tissue is very popular and many techniques have been described, there are still no accepted protocols, definition for the end product, and no consensus on the status of the stromal cells. In this study, stromal cells were obtained mechanically by using ultra-sharp blade systems, without exposing adipose tissue to blunt trauma. Thus, a higher number of cells and higher viability could be obtained. An “Indication based” protocol has been defined for the first time in order to obtain the desired number and status (solid, semi-solid, liquid) end product. Diluting the condensed adipose tissue with the desired solutions (saline, Ringer or 5% Dextrose) before the adinizing process will provide even more stromal cells. This will provide an opportunity for clinicians to obtain and apply a stromal cell solution for different indications in different anatomical regions.

show abstract