MEK4 Function, Genistein Treatment, and Invasion of Human Prostate Cancer Cells

Xu, Li; Ding, Yongzeng; Catàlona, William J.; Yang, Ximing J.; Anderson, W.F.; Jovanovic, Borko; Wellman, K.; Killmer, Jaqueline; Huang, Xiaoke; Scheidt, Karl A.; Montgomery, Robert B.; Bergan, Raymond C.

doi:10.1093/jnci/djp227

Cited by 99 publications

(114 citation statements)

References 54 publications

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“…[1][2][3][4][5][6][7][8] Nevertheless, concerns have been raised about the use of cell lines that can be divided into two categories of criticism. First, mainly due to poor experimental conduct, false (derived from another cell population within the cell culture than the one intended), cross-contaminated (with other human cells or cells from other species) and/or pathogen-(mycoplasm) contaminated cell lines have been discovered repeatedly.…”

mentioning

confidence: 99%

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

Mohseny

Machado

Cai

et al. 2011

Laboratory Investigation

163

151

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Cancer cell lines represent in vitro models for studying malignancies, general cell biology, drug discovery and more. Whether they can be considered as exact representative models of the parental tumors remains uncertain given the acquisition of additional ex vivo changes of the cells and the lack of tissue architecture and stroma. Previously, within the EuroBoNeT consortium, we characterized a collection of bone sarcoma cell lines on genomic and proteomic level. Here, we address the phenotypical and functional characterization of the unique set of osteosarcoma cell lines (n ¼ 19) in vitro and in vivo. For functional analysis of differentiation capacity, cells were stimulated towards osteoblasts, adipocytes and chondrocytes. Furthermore, all cell lines were injected subcutaneously and intramuscularly into nude mice to assay their in vivo tumor formation capacity as well as for phenotypical analysis of the tumors. All formed tumors were further characterized histologically and immunohistochemically. Out of 19 cell lines, 17 (89%) showed adipogenic differentiation, 13/19 (68%) could differentiate towards osteoblasts and in 6/19 (32%) cell lines chondrogenic differentiation was evident. About half of the cell lines (8/19, 42%) produced tumors in vivo after subcutaneous and intramuscular injections. Several cell lines showed invasion into adjacent tissues and one tumor developed several lung metastases. The use of cell lines, especially in cancer research, is of paramount importance. Here, we identify comprehensively characterized osteosarcoma cell lines, which robustly represent clinical osteosarcoma providing researchers useful in vitro and in vivo models to study the genetics and functional characteristics of this highly malignant neoplasm.

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confidence: 99%

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

Mohseny

Machado

Cai

et al. 2011

Laboratory Investigation

163

151

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“…Genistein is considered that inhibit cell proliferation through regulation of MAPK [28][29][30] , and inhibit phosphorylation of ERK and Akt [31][32][33] . The high concentration group (G100) showed suppress the cell proliferation after 3 days incubation.…”

Section: Discussionmentioning

confidence: 99%

The possibility of genistein as a new direct pulp capping agent

et al. 2013

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Genistein, kind of soy isoflavones, is well-known as natural ingredients and consumed as health foods and supplements. They are expected to improve renal function. They have high-affinity to estrogen receptor β expressed predominantly in bone tissue, they prevent osteoporosis specifically and safely. We examined whether genistein can be a new direct capping agent. In this study, we examined the effect of genistein for the proliferation and differentiation of rat dental pulp cells in vitro and the ability of tertiary dentin formation in vivo. As a result, rat dental pulp cells with genistein were increased activity of ALPase and showed alizarin red positive-staining. Calcification-related genes expression has been confirmed by the addition of genistein. From in vivo study, high quality of tertiary dentin formation and minor pulp reaction were observed. From these findings, it was suggested that genistein may be useful agent for direct pulp capping.

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“…Kousidou et al demonstrated that genistein suppresses the invasive potential of human breast cancer cells through the transcriptional regulation of metalloproteinases and their tissue inhibitors (19). Results by Huang et al revealed that genistein inhibits p38 MAP kinase activation, matrix metalloproteinase type 2, and cell invasion in human prostate epithelial cells (20,21). Lakshman et al also reported that dietary genistein inhibited the metastasis of human prostate cancer in mice (22).…”

Section: Discussionmentioning

confidence: 99%

Genistein inhibits placental choriocarcinoma cell line JAR invasion through ERβ/MTA3/Snail/E-cadherin pathway

Liu

et al. 2011

Oncol Lett

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Abstract. Genistein, the most abundant phytoestrogen in soybeans, may bind to estrogen receptors and perform anticancer activities. Choriocarcinoma is a malignant, trophoblastic and aggressive cancer of the placenta. Few studies are currently available concerning the effects of genistein on choriocarcinoma. In the present study, we investigated the effect of genistein on the invasive potential of the choriocarcinoma cell line JAR and its underlying mechanism. Our data revealed that genistein inhibited JAR cell invasion in a dose-dependent manner by a matrigel invasion assay. Moreover, genistein was found to have decreased the metastasis-associated gene MTA3 mRNA level, increased the transcriptional suppressor Snail mRNA level and upregulated the protein expression of the cell-cell adhesion molecule E-cadherin by real-time RT-PCR and Western blot analysis, respectively. ERβ siRNA was used to knock down ERβ expression in JAR cells. In the ERβ-knockdown JAR cells, genistein failed to inhibit JAR cell invasion. The effects of genistein on MTA3, Snail and E-cadherin expression were also eradicated following ERβ siRNA transfection. These results demonstrated that genistein triggered the MTA3/Snail/E-cadherin regulatory pathway by binding with ERβ, thereby inhibiting JAR cell invasion. In conclusion, our findings have significant implications for the prevention and therapy of choriocarcinoma.

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MEK4 Function, Genistein Treatment, and Invasion of Human Prostate Cancer Cells

Cited by 99 publications

References 54 publications

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

The possibility of genistein as a new direct pulp capping agent

Genistein inhibits placental choriocarcinoma cell line JAR invasion through ERβ/MTA3/Snail/E-cadherin pathway

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