Meiotic Nondisjunction in the Mouse: Methodology for Genetic Testing and Comparison with Other Methods

Russell, Liane B.

doi:10.2307/3429148

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Germ cells in the fetal as well as the adult ovar,y are potential targets for cytogenetic toxicity. Aneuploiqy induction could possibly involve toxicant exposure at any of the three stages: 1) during early oogenesis causing reduced chiasmata frequencies; 2) during meiotic division leading to spindle damage; or 3) throughout oogenesis whenever the kinetochore is susceptible to damage (Russell, 1979).…”

Section: The Effects Of Metals On Oocyte Maturation Fertilization Anmentioning

confidence: 99%

Reproductive and Developmental Toxicity of Metals: Female Reproductive System

Mattison

Gates

Léonard³

et al. 1983

Reproductive and Developmental Toxicity of Metals

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Section: The Effects Of Metals On Oocyte Maturation Fertilization Anmentioning

confidence: 99%

Reproductive and Developmental Toxicity of Metals: Female Reproductive System

Mattison

Gates

Léonard³

et al. 1983

Reproductive and Developmental Toxicity of Metals

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Aneuploidy and health risk assessment: Current status and future directions

1985

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The U.S. Environmental Protection Agency (EPA) recently sponsored a workshop to discuss the contribution of aneuploidy to human disease and disability, the development of tests for detecting chemicals that induce aneuploidy and the relevance of these tests to human risk, and the current understanding of mechanisms by which aneuploidy arises. This summary is based on the presentations given at the workshop. It is hoped that this summary will stimulate thinking in this vitally important area of risk assessment and contribute to the establishment of priorities for basic research, development of new test methods, and validation of existing test approaches. Such research is needed to enhance the scientific basis of risk assessment for aneuploidy-producing chemicals.

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Oocyte aneuploidy screening using superovulating prepubertal mice: Effect of methotrexate

1981

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Numerical chromosomal aberrations (aneuploidy) are a major factor in pregnancy wastage, birth defects, and mental retardation. Consequently, effective cytogenetic procedures in mammalian gamates are required for studying mechanisms and causes of chromosomal nondisjunction. Our aims were to determine for oocytes from superovulating prepubertal mice: (1) the yield of chromosomally scorable ova, following application of a double fixation procedure: (2) the background level of aneuploidy, and (3) the sensitivity to induction of aneuploidy (by methotrexate). Superovulation was induced in C129F1 hybrid mice, 22-24 days old, with pregnant mare serum and human chorionic gonadotropin (HCG). Diurnal photoperiodicity and injections were scheduled to assure HCG-induced ovulation of known timing. Methotrexate (200 mg/kg) was given at 3 hr and ova were recovered at 15 hr after HCG. We describe the adaptation of a double fixation procedure to mouse oocytes. Methotrexate led to significantly increased hypoploidy (2 1/2-fold) but not to the hyperploidy reported by others for adult mice. There was a high yield of ova with exactly countable chromosomes (average of 9.5 ova per mouse). Concomitantly, the background level of aneuploidy was very low (0/465 scorable ova were hyperploid). Given the additional advantages of economy and convenience, the superovulating 3-week-old mouse could be an effective source of ova for testing environmental agents for their aneuploidy-inducing potential; however, further studies are needed to establish the degree to which such ova are susceptible to aneuploidy induction.

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Meiotic Nondisjunction in the Mouse: Methodology for Genetic Testing and Comparison with Other Methods

Cited by 3 publications

References 29 publications

Reproductive and Developmental Toxicity of Metals: Female Reproductive System

Reproductive and Developmental Toxicity of Metals: Female Reproductive System

Aneuploidy and health risk assessment: Current status and future directions

Oocyte aneuploidy screening using superovulating prepubertal mice: Effect of methotrexate

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