Meiotic maturation and in vitro maturation of bovine oocytes

Abstract

Contents: In vitro production of embryos, and, as part of this method, in vitro maturation of oocytes, have received great attention in last ten-fifteen years. It is well established in bovine. Here, in this review is presented importance of this method, usual meiotic division is described, as well, as importance of biochemical investigations of several protein factors and enzymes, which control these processes.

Using RT-PCR and glutathione level to study the effect of follicular fluid on in vitro maturation and gene expression of sheep oocytes

“…Although oocytes matured in vitro and in vivo have similar rates of nuclear maturation, fertilization and cleavage, they clearly differ in their developmental potential (Blondin and Sirard, 1995, Dieleman et al, 2002, Dunning et al, 2007, Kyasari et al, 2012, Rizos et al, 2002). Thus, the blastocyst rate after in vitro oocyte maturation, fertilization and embryo culture is 30–40%, whereas 70% of in vivo mature oocytes develop into blastocysts (Dieleman et al, 2002, Humblot et al, 2005, Rizos et al, 2002, Romar et al, 2011, Smiljakovic and Tomek, 2006, van de Leemput et al, 1999). Thus, several substances have been added to test different culture conditions for IVM.…”