Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan.

Chen, Mao-Yen; Lin, Guang-Huey; Lin, Yung-Ting; Tsay, San-San

doi:10.1099/00207713-52-5-1647

Cited by 24 publications

(25 citation statements)

References 31 publications

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“…Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were obtained from hot springs in Taiwan (12,13,30). All biochemical tests and identification procedures followed previously described methods (6,30).…”

Section: Methodsmentioning

confidence: 99%

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Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus

Yang

Jao

et al. 2006

Journal of Lipid Research

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The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 29-O-(1,2-diacyl-sn-glycero-3-phospho) -39-O-(a-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-Ophosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C 15:0 , -C 16:0 , and -C 17:0 and anteiso-C 15:0 and -C 17:0 . The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso-to anteiso-branched fatty acids increase when grown at the higher temperature. Supplementary key words nuclear magnetic resonance spectroscopy . matrix-assisted laser desorption ionization mass spectroscopy . capillary electrophoresis-mass spectroscopy . tandem mass spectrometry . Thermus oshimai NTU-063 . Thermus thermophilus NTU-077 . Meiothermus ruber NTU-124 . Meiothermus taiwanensis NTU-220Bacteria have evolved interesting thermoadaptive mechanisms, including changes in their membranes (1). For example, when Escherichia coli, Thermus aquaticus, Candida species, thermophilic Bacillus species, and Staphylococcus aureus are grown at high temperatures, the proportion of branched-chain fatty acids increases and the proportions of monoenoic and heptanoic fatty acids decrease (2). Temperature affects both the chain length and the degree of saturation of fatty acid components in Synechococcus species (3, 4). Polar lipid fatty acids can be used as a biochemical marker because many of the lipids of thermophilic bacteria isolated from microbial mats in hot springs have unique diol, plasmalogen, monoether, and diether structures (5).Thermus and Meiothermus species are Gram-negative thermophilic rods isolated from thermal hot springs, industrial and domestic water traps, and hydrothermal vents with neutral to alkaline pH (6). In these species, polar lipids occupy a large proportion of the cellular membrane fractions and usually include a major phospholipid, a major glycolipid, and minor phospholipids and glycolipids (7,8). The glycolipids usually contain three hexoses, one N-hexosamine, and one glycerol (6, 9-13). The hydrophobic parts are predominantly iso-and anteiso-branched fatty acids; straight-chain fatty acids are minor components. The high proportion of glycolipids in the cell membranes could possibly contribute to the ability of the bacteria to grow at high temperatures, because the relative proportions of the major glycolipids increase concomitantly with the growth temperature (14, 15).Thermus and Meiothermus species have been reported to have phosphoglycolipids (16) Abbreviations: CE-MS, capillary electrophoresis-mass spectroscopy; DQF-COSY, double quantum filtered-c...

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Section: Methodsmentioning

confidence: 99%

“…All biochemical tests and identification procedures followed previously described methods (6,30). The voucher specimens (Thermus NTU-063, Thermus NTU-077, Meiothermus NTU-124, and Meiothermus NTU-220) were deposited at the Institute of Biological Chemistry, Academia Sinica (Taipei, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus

Yang

Jao

et al. 2006

Journal of Lipid Research

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“…However, based on differences in optimal growth temperature range, 2-hydroxy fatty acid content and 16S rRNA gene sequences, they were later reclassified within a separate genus (Nobre et al, 1996). At the time of writing, the genus Meiothermus comprises nine recognized species (Albuquerque et al, 2009(Albuquerque et al, , 2010Chen et al, 2002;Chung et al, 1997;Pires et al, 2005;Zhang et al, 2010). Many strains belonging to the genus Meiothermus have been isolated from various natural geothermal samples as well as from man-made environments (Ekman et al, 2007).…”

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Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Mori

Iino

Ishibashi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A novel moderately thermophilic, red-pigmented bacterium, designated strain AZM34c11 T , was isolated from the bottom of a 1000 m-deep drilled well located in a Japanese hot spring. Cells were Gram-negative and grew optimally at 50 6C, at pH 7.6 and with 0-0.3 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that the isolate belonged to the genus Meiothermus. Levels of 16S rRNA gene sequence similarity between strain AZM34c11 T and the type strains of recognized Meiothermus species were 88.2-94.8 %. Strain AZM34c11 T was distinguished from recognized Meiothermus species by its cellular fatty acid profile: iso-C 16 : 0 was one of the predominant components and hydroxy fatty acids were present only in trace amounts. The strain could also be differentiated based on its growth substrate preferences and characteristic enzyme reactions. On the basis of these results, strain AZM34c11 T is considered to represent a novel species of the genus Meiothermus, for which the name Meiothermus hypogaeus sp. nov. is proposed. The type strain is AZM34c11 T (5NBRC 106114 T 5DSM 23238 T ).

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“…Isolation and purification of the glycolipids M. taiwanensis ATCC BAA-400 (Wu-rai hot spring, Taiwan) was grown aerobically in Thermus modified medium [14,16] at 55°C and harvested until the late exponential phase (D 660 ¼ 1.6). A suspension of wet bacteria in absolute ethanol (1 : 10, w/v, Riedel-de-Hae¨n, Germany) was shaken at room temperature for 2 h. After centrifugation, the supernatant was collected, concentrated, and purified through a Sephadex LH-20 column (Amersham Pharmacia, 80 · 1.1 cm) eluted with methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Although the sequences of those carbohydrate moieties have been studied by chemical and mass spectroscopic analysis, no complete structure is available as yet due to the lack of information on the linkages and configurations of the carbohydrate moiety. We have been working on a newly discovered species of thermophilic bacteria, Meiothermus taiwanensis, recently isolated from the Wu-rai hot spring in Taiwan [16], as part of our program to investigate the immunomodulation activity of the glycolipids and the structure-activity relationship. In this study, we determined the structure of a major glycolipid from the thermophilic bacteria M. taiwanensis ATCC BAA-400.…”

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Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis

Yang

Chen³

et al. 2004

European Journal of Biochemistry

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The polar glycolipids were isolated from the thermophilic bacteria Meiothermus taiwanensis ATCC BAA-400 by ethanol extraction and purified by Sephadex LH-20 and silica gel column chromatography. The fatty acid composition of O-acyl groups in the glycolipids was obtained by gas chromatography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of C 15:0 (34.0%) and C 17:0 (42.3%) fatty acids, with the majority as branched fatty acids (over 80%). Removal of O-acyl groups under mild basic conditions provided two glycolipids, which differ only in N-acyl substitution on a hexosamine. Electrospray mass spectroscopy analysis revealed that one has a C 17:0 N-acyl group and the other hydroxy C 17:0 in a ratio of about 1 : 3.5. Furthermore, complete de-lipidation with strong base followed by selective N-acetylation resulted in a homogeneous tetraglycosyl glycerol. The linkages and configurations of the carbohydrate moiety were then elucidated by MS and various NMR analyses. Thus, the major glycolipid from M. taiwanensis ATCC BAA-400 was determined with the following structure:where N-acyl is C 17:0 or hydroxy C 17:0 fatty acid and the glycerol esters were mainly iso-and anteisobranched C 15:0 and C 17:0 .Keywords: glycolipid; Meiothermus taiwanensis; MS; NMR; thermophilic bacteria.The thermophilic bacteria such as Aquifex pyrophilus, Thermodesulfotobacterium commune, Thermus scotoductus, Thermomicrobium roseum and Thermodesulfatator indicus contain unique polar lipids as major membrane components [1][2][3][4][5][6][7][8]. Those lipids are essential for the thermal stability and biological functions of the bacteria in extreme environments [9][10][11]. The polar lipids found in Thermus aquaticus, Thermus filiformis, Thermus scotoductus, and Thermus oshimai were mostly phospholipids and glycolipids [12], and the glycolipids from Thermus species examined thus far usually contain three hexoses, one N-hexosamine, and one glycerol [7,10,[12][13][14][15]. Although the sequences of those carbohydrate moieties have been studied by chemical and mass spectroscopic analysis, no complete structure is available as yet due to the lack of information on the linkages and configurations of the carbohydrate moiety. We have been working on a newly discovered species of thermophilic bacteria, Meiothermus taiwanensis, recently isolated from the Wu-rai hot spring in Taiwan [16], as part of our program to investigate the immunomodulation activity of the glycolipids and the structure-activity relationship. In this study, we determined the structure of a major glycolipid from the thermophilic bacteria M. taiwanensis ATCC BAA-400. The fatty acids were examined by gas chromatography mass spectroscopy (GC-MS) analysis on their methyl esters derived from methanolysis, whereas, the structure of the carbohydrate moiety was elucidated by MS/MS and NMR spectroscopic analyses. To the best of our knowledge this is the first complete glycolipid structure from thermophilic bacteria. Materials and methodsIsolation and...

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Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan.

Abstract: Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan

Cited by 24 publications

References 31 publications

Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus

Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus

Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis

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