Meiosis in<i>Allium fistulosum, Allium cepa</i>, and their hybrid

Emsweller, S. L.; Jones, Howard A.

doi:10.3733/hilg.v09n05p275

Cited by 24 publications

(20 citation statements)

References 9 publications

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“…The frequency of homoeologous chromosome pairing and recombination is quite high in the meiosis of F 1 hybrids between these two species (Emsweller and Jones, 1935;Maeda, 1937;Levan, 1941;Cochran, 1950;Tashiro, 1984;Peffley, 1986). In the present study, the two of single-alien deletion plants which belonged in the subgroup 7 and 8 showed disagreement between cytogenetics and molecular data.…”

Section: Discussionmentioning

confidence: 99%

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

et al. 2009

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To develop the bunching onion (Allium fistulosum L.; genomes, FF) chromosomespecific genetic markers for identifying extra chromosomes, eight shallot (A. cepa L. Aggregatum group; genomes, AA) -A. fistulosum monosomic addition plants (AA+nF) and 62 shallot -A. fistulosum single-alien deletion plants (AAF-nF) were analyzed by 23 different chromosome-specific genetic markers of shallot. The eight monosomic addition plants consisted of one AA+2F, two AA+6F, and five AA+8F. Of the 62 single-alien deletion plants, 60 could be identified as six different single-alien deletion lines (AAF-1F, -3F, -4F, -6F, -7F, and -8F) out of the eight possible types. Several single-alien deletion lines were classified on the basis of leaf and bulb characteristics. AAF-8F had the largest number of expanded leaves of five deletion plants. AAF-7F grew most vigorously, as expressed by its long leaf blade and biggest bulb size. AAF-4F had very small bulbs. AAF-7F and AAF-8F had different bulbs from those of shallot as well as other types of single-alien deletion lines in skin and outer scale color. Regarding the sugar content of the bulb tissues, the single-alien deletion lines showed higher fructan content than shallot. Moreover, shallot could not produce fructan with degree of polymerization (DP) 12 or higher, although the single-alien deletion lines showed DP 20 or higher. The content of S-alk(en)yl-L-cysteine sulfoxide (ACSO) in the single-alien deletion lines was significantly lower than that in shallot. These results indicated that chromosomes from A. fistulosum might carry anonymous factors to increase the highly polymerized fructan production and inhibit the synthesis of ACSO in shallot bulbs. Accordingly, alien chromosomes from A. fistulosum in shallot would contribute to modify the quality of shallot bulbs.

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Section: Discussionmentioning

confidence: 99%

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

et al. 2009

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“…Jones, 1938). Emsweller and Jones (1935a, 1935b, 1938 as well as Peffley (1986) and Maeda (1937) reported regular bivalentization (Fig. 2) in about 70% of tested cells, whereas Levan (1941) reported regular bivalent formation in only 2% of the tested PMC in his A. cepa x A. fistulosum hybrids.…”

Section: Hybrids With Introgression From a Fistulosummentioning

confidence: 75%

“…Hence, hybrids between onion and A. fistulosum as well as several backcross progenies were deeply studied, with A. cepa as either the female parent (Emsweller & H.A. Jones, 1935a, 1935bLevan, 1941;Van der Meer and Van Benekom, 1978) or pollen parent (Peffley, 1986;Peters et al, 1984;van der Valk et al 1991a). Cytological studies have shown that chromosome pairing is highly variable in the F 1 hybrid depending on the parental combination (Emsweller & H.A.…”

Section: Discussionmentioning

confidence: 99%

“…When the bivalents were formed by chromosomes of different length, they sometimes separated in association long plus long chromatid, as well as long plus short chromatid. Emsweller and Jones (1935b) observed extension of long arm of conjugated chromosomes well beyond the end of the other. That condition depends on the arrangement of chiasmata at the ends of a bivalent (Emsweller & H.A.…”

Section: Hybrids With Introgression From a Fistulosummentioning

confidence: 95%

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Meiotic Irregularities in Interspecific Crosses Within Edible Alliums

Kiełkowska¹

2012

Meiosis - Molecular Mechanisms and Cytogenetic Diversity

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“…This result shows that in each addition line an entire (unrecombined) A. cepa (shallot) chromosome is present in an integral diploid background of A. fistulosum. Since the frequency of homoeologous pairing and recombination is quite high in the meiosis of F 1 hybrids between these two species (Emsweller and Jones, 1935;Maeda, 1937;Levan, 1941;Cochran, 1950;Tashiro, 1984;Peffley, 1986), this implies a considerable degree of selection for entire chromosomes in the process of constructing the monosomic addition lines. Our monosomic addition lines were selected from the backcross progenies of interspecific triploids (2 × A. fistulosum + 1 × shallot).…”

Section: Resultsmentioning

confidence: 99%

Identification of alien chromosomes in a series of Allium fistulosum. A. cepa monosomic addition lines by means of genomic in situ hybridization.

et al. 1998

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Genomic in situ hybridization (GISH) was used to identify alien chromosomes in a series of eight different Allium fistulosum -A. cepa monosomic addition lines. Biotin-labeled total genomic DNA from shallot (A. cepa L. Aggregatum group) was used as a probe together with an excess amount of unlabeled blocking DNA from the recipient plant, Japanese bunching onion (A. fistulosum L.). Probe hybridization sites were detected by FITC-conjugated avidin and anti-avidin antibody using an epifluorescence microscope. In each mitotic metaphase cell of all the eight types of monosomic addition lines, the alien chromosomes were successfully discriminated from other 16 A. fistulosum chromosomes. Furthermore, no clear exchanges of chromosome segments between A. cepa and A. fistulosum were observed. This finding indicates that in each addition line an entire (unrecombined) A. cepa chromosome is present in an integral diploid background of A. fistulosum.

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Meiosis inAllium fistulosum, Allium cepa, and their hybrid

Cited by 24 publications

References 9 publications

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

Meiotic Irregularities in Interspecific Crosses Within Edible Alliums

Identification of alien chromosomes in a series of Allium fistulosum. A. cepa monosomic addition lines by means of genomic in situ hybridization.

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