Megaplasmids and the Degradation of Aromatic Compounds by Soil Bacteria

Vedler, Eve

doi:10.1007/978-3-540-85467-8_2

Cited by 10 publications

(7 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1) com parable in size with the pRHL2 (443 kb) and pRHL3 (332 kb) megaplasmids of the R. jostii RHA1 active destructor of aromatic compounds [25]. It should be noted that the plasmid profile of the strain KT112 7 did not change during cultivation in both a RRM and a RMM with aromatic substrates: biphenyl, BA, and o PA (the data are not shown).…”

Section: Methodsmentioning

confidence: 88%

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

Егорова

Korsakova

Демаков

et al. 2013

Appl Biochem Microbiol

View full text Add to dashboard Cite

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112 7 isolated from technogenic mineral waste products of the BKRU1 "Uralkalii" plant was investigated (Berezniki, Perm krai). The R. wratislaviensis KT112 7 was shown to utilize increased concentrations of o phthalic (o PA) (8 g/L) and benzoic (BA) (3.4 g/L) acids. The strain grows with o PA, BA, and biphenyl at a NaCl content of up to 75, 90 and 100 g/L in the culture medium, respectively. Based on an analysis of the metabolic profile and nucleotide sequences of the bphA1, benA, and phtB genes, the strain KT112 7 was found to degrade o PA via the formation of 3,4 dihydroxyphthalic and 3,4 dihydroxybenzoic acids. Degradation of biphenyl proceeds via the formation of BA and then at low concentrations of NaCl (up to 50 g/L) via the formation of 4 hydroxy benzoic acid with its subsequent oxidation, while at high concentrations of NaCl (over 60 g/L)-via the direct oxidation of benzoic acid to resultant catechol. These data indicate that the Rhodococcus wratislaviensis KT112 7 is a promising strain in the development of new biotechnologies directed for utilization (transfor mation) of aromatic compounds, including the conditions of increased mineralization.

show abstract

Section: Methodsmentioning

confidence: 88%

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

Егорова

Korsakova

Демаков

et al. 2013

Appl Biochem Microbiol

View full text Add to dashboard Cite

show abstract

“…Until now, no less than 37 plasmid-encoded pathways for metabolism of aromatic compounds were described (Vedeler 2009), as well as plasmids for the degradation of rubber (Bröker & Steinbüchel 2009), phthalate (Eaton 2001) or naphthalene (Rosselló-Mora et al 1994). These genetic elements thus allow the bacteria to grow in many contaminated environments as natural autochthonous microflora with a high potential for bioremediation of pollutants.…”

Section: Introductionmentioning

confidence: 99%

In-silico evidence of a pAO1 encoded pathway for the catabolism of tagatose derivatives in Arthrobacter nicotinovorans

Mihășan

2010

Biologia

View full text Add to dashboard Cite

Based on similarity searches, two putative pathways were previously described as being encoded by the pAO1 megaplasmid of Arthrobacter nicotinovorans: an almost fully established nicotine-degrading pathway and a yet unknown putative sugar-catabolic pathway. The general organization of the open reading frames (ORFs) of the latter indicated possible gene products as targets for docking experiments, aimed at identifying possible sugar substrates of this pathway. Homology modelling and docking results with the deduced proteins of three ORFs of the putative sugar catabolic pathway indicated D-tagatose-1,6-bisphosphate as a common ligand and thus as substrate of the pathway.

show abstract

“…Besides using their phenotypic plasticity, bacteria are capable of transferring single functional genes or whole gene clusters within and between species. Such horizontal gene transfer (HGT) is a main driver of the evolution of bacteria34. Conjugation, i.e.…”

mentioning

confidence: 99%

Mycelia as a focal point for horizontal gene transfer among soil bacteria

Berthold

Centler

Hübschmann

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Horizontal gene transfer (HGT) is a main mechanism of bacterial evolution endowing bacteria with new genetic traits. The transfer of mobile genetic elements such as plasmids (conjugation) requires the close proximity of cells. HGT between genetically distinct bacteria largely depends on cell movement in water films, which are typically discontinuous in natural systems like soil. Using laboratory microcosms, a bacterial reporter system and flow cytometry, we here investigated if and to which degree mycelial networks facilitate contact of and HGT between spatially separated bacteria. Our study shows that the network structures of mycelia promote bacterial HGT by providing continuous liquid films in which bacterial migration and contacts are favoured. This finding was confirmed by individual-based simulations, revealing that the tendency of migrating bacteria to concentrate in the liquid film around hyphae is a key factor for improved HGT along mycelial networks. Given their ubiquity, we propose that hyphae can act as focal point for HGT and genetic adaptation in soil.

show abstract

Megaplasmids and the Degradation of Aromatic Compounds by Soil Bacteria

Cited by 10 publications

References 78 publications

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

In-silico evidence of a pAO1 encoded pathway for the catabolism of tagatose derivatives in Arthrobacter nicotinovorans

Mycelia as a focal point for horizontal gene transfer among soil bacteria

Contact Info

Product

Resources

About