Megakaryoblastic leukemia in an infant: Establishment of a megakaryocytic tumor cell line in athymic nude mice

Abstract

A 17-month-old infant with clinical and pathologic features of acute megakaryoblastic leukemia and myelofibrosis developed soft tissue metastases. Tissue from an orbital metastasis was biopsied and transplanted into a nude mouse. Histologically the tumor was composed of pleomorphic cells with single convoluted nuclei or multilobed nuclei and prominent granular cytoplasm and had an alveolar histologic pattern in some areas. The ultrastructural features of the tumor cells include multilobed nuclei with prominent nucleoli and cytoplasmic granules with the characteristics of alpha-granules. The tumor has been successfully passaged over a 1-year interval and appears to be a stable megakaryoblastic tumor cell line (CHRF-288). Cells from the tumor line are reactive for factor VIII related antigen and also have GpIIb IIIa complex antigen on the plasma membrane surface. This tumor line may be a useful system for investigation of megakaryocytic functions including the production and regulation of the various factors they produce.

Maps of Open Chromatin Guide the Functional Follow-Up of Genome-Wide Association Signals: Application to Hematological Traits

“…Both cell lines exhibit markers characteristic for megakaryocytes/platelets [40], [41] and erythroblasts [42], [43], respectively. CHRF-288-11 cells were maintained in RPMI-1640 medium, supplemented with 20% horse serum (heat-inactivated) and 1% L-glutamine-penicillin-streptomycin solution.…”

JAK2T875N is a novel activating mutation that results in myeloproliferative disease with features of megakaryoblastic leukemia in a murine bone marrow transplantation model

“…CHRF-288-11 was originally established from a biopsy of a granulocytic sarcoma in a 17-month-old infant with AMKL and myelofibrosis. 43 CHRF-288-11 differs from a number of other cell lines derived from patients with AMKL in its expression of megakaryocyte markers, but not erythroid markers. 44 In addition to the JAK2T875N mutation described in this report, CHRF-288-11 presents several chromosomal abnormalities including several that are often identified in patients with AMKL, including trisomy 21 and trisomy 19.…”

Calcium adaptation to sodium pump inhibition in a human megakaryocytic cell line

“…Excitation wavelengths were set at 340 and 385 nm, and emission wavelength was set at 505 nm. Calibration of Na c was accomplished by the gramicidin method as previously described (23). Gramicidin D (2 mol/l), monensin (10 mol/l), and nigericin (10 mol/l) were added to calibration solutions consisting of (in mmol/l) 0 -115 Na gluconate, 0 -115 K gluconate, 30 KCl, 1 CaCl 2, 1 MgCl2, 10 glucose, and 10 HEPES (pH 7.4).…”