Calcium adaptation to sodium pump inhibition in a human megakaryocytic cell line

Kimura, Masayuki; Cao, Xiaojian; Aviv, Abraham

doi:10.1152/ajpcell.00080.2005

Cited by 5 publications

(4 citation statements)

References 26 publications

(18 reference statements)

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“…by PCr breakdown (Adams et al 1990;Bendahan et al 2003). [It has been pointed out before (Kemp 2005) that approximate treatments which over-simplify the charge stoichiometry (Kemp 2005;Kemp et al 2006;Kushmerick 1997;Lindinger et al 2005;Robergs et al 2004) cannot account for this alkalinisation, which results from the difference in charge between PCr and Pi at physiological pH (Kemp 2005;Kemp et al 2006;Kushmerick 1997;Lindinger et al 2005;Robergs et al 2004)]. Secondly, we can conclude that recovery data (Fig.…”

Section: Discussionmentioning

confidence: 74%

“…Glycolytic ATP turnover produces what one can think of as 1 H ? per lactate (Kemp 2005) [this approach to cellular acid-base balance is in this context quantitatively compatible ) with the alternative 'physiochemical' approach (Lindinger et al 2005)]. If H ?…”

Section: Methodsmentioning

confidence: 94%

“…is generated by post-exercise PCr resynthesis at a pH lower than that at which most H ? is consumed during exercise; as the stoichiometric coefficient increases in magnitude with decreasing pH (Kushmerick 1997) [because of the relatively greater pH-dependence of the smaller charge on Pi, compared to PCr (Kemp 2005;Kemp et al 2006;Kushmerick 1997;Lindinger et al 2005;Robergs et al 2004)], H ? generated in recovery slightly exceeds H ?…”

Section: Discussionmentioning

confidence: 99%

“…by the resynthesis of ATP at the expense of phosphocreatine (PCr), and moderated by various membrane transport processes (Juel 2008). The concepts of intracellular acid-base physiology are still a matter of some debate (Kemp 2005;Kemp et al 2006;Kushmerick 1997;Lindinger et al 2005;Robergs et al 2004). Furthermore, data on cellular pH-buffering in skeletal muscle are relatively limited (Kemp et al 2001b) [compared to e.g.…”

Section: Introductionmentioning

confidence: 99%

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Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle’s disease

et al. 2008

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Cellular pH control is important in muscle physiology, and for interpretation of (31)P magnetic resonance spectroscopy (MRS) data. Cellular acidification in exercise results from coupled glycolytic ATP production mitigated by cytosolic buffering, 'consumption' of H(+) by phosphocreatine (PCr) breakdown, and membrane transport processes. Ex vivo methods for cytosolic buffer capacity are vulnerable to artefact, and MRS methods often require assumptions. (31)P MRS of early exercise, when pH increases unopposed by glycolysis, is conceptually simple, but limited in normal muscle by time resolution and signal-to-noise. A therapeutic trial (Martinuzzi A et al. Musc Nerve 37: 350-357, 2007) in McArdle's disease (glycogen phosphorylase deficiency), where pH does not decrease with exercise, offered the opportunity to test (31)P MRS data obtained throughout incremental plantar flexion exercise and recovery in ten McArdle's patients against the simple model of cellular pH control. Changes in pH, [Pi] and [PCr] throughout exercise and recovery were quantitatively consistent with mean +/- SEM buffer capacity of 10 +/- 1 mM/(pH unit), which was not significantly different from the control subjects under the initial-exercise conditions where the comparison could be made. The simple model of cellular acid-base balance therefore gives an adequate account of cellular pH changes during both exercise and recovery in McArdle's disease.

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Section: Discussionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle’s disease

et al. 2008

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Microneedles for Transdermal Biosensing: Current Picture and Future Direction

Ventrelli

Strambini

Barillaro

2015

Adv Healthcare Materials

186

126

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A novel trend is rapidly emerging in the use of microneedles, which are a miniaturized replica of hypodermic needles with length-scales of hundreds of micrometers, aimed at the transdermal biosensing of analytes of clinical interest, e.g., glucose, biomarkers, and others. Transdermal biosensing via microneedles offers remarkable opportunities for moving biosensing technologies and biochips from research laboratories to real-field applications, and envisages easy-to-use point-of-care microdevices with pain-free, minimally invasive, and minimal-training features that are very attractive for both developed and emerging countries. In addition to this, microneedles for transdermal biosensing offer a unique possibility for the development of biochips provided with end-effectors for their interaction with the biological system under investigation. Direct and efficient collection of the biological sample to be analyzed will then become feasible in situ at the same length-scale of the other biochip components by minimally trained personnel and in a minimally invasive fashion. This would eliminate the need for blood extraction using hypodermic needles and reduce, in turn, related problems, such as patient infections, sample contaminations, analysis artifacts, etc. The aim here is to provide a thorough and critical analysis of state-of-the-art developments in this novel research trend, and to bridge the gap between microneedles and biosensors.

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Membrane Tension Modulates the Effects of Apical Cholesterol on the Renal Epithelial Sodium Channel

Wei

Chou

et al. 2007

J Membrane Biol

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We used patch-clamp techniques and A6 distal nephron cells as a model to determine how cholesterol regulates the renal epithelial sodium channel (ENaC). We found that luminal methyl-beta-cyclodextrin (mbetaCD, a cholesterol scavenger) did not acutely affect ENaC activity at a previously used concentration of 10 mM: but significantly decreased ENaC activity both when the cell membrane was stretched and at a higher concentration of 50 mM: Luminal cholesterol had no effect on ENaC activity at a concentration of 50 microg/ml but significantly increased ENaC activity both when the cell membrane was stretched and at a higher concentration of 200 microg/ml. Confocal microscopy data indicate that membrane tension facilitates both mbetaCD extraction of cholesterol and A6 cell uptake of exogenous cholesterol. Together with previous findings that cholesterol in the apical membrane is tightly packed with sphingolipids and that stretch can affect lipid distribution, our data suggest that membrane tension modulates the effects of mbetaCD and cholesterol on ENaC activity, probably by facilitating both extraction and enrichment of apical cholesterol.

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Calcium adaptation to sodium pump inhibition in a human megakaryocytic cell line

Cited by 5 publications

References 26 publications

Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle’s disease

Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle’s disease

Microneedles for Transdermal Biosensing: Current Picture and Future Direction

Membrane Tension Modulates the Effects of Apical Cholesterol on the Renal Epithelial Sodium Channel

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