Megacolon in Rats

Oliveira, Julicristie Machado de; Llorach-Velludo, Maria A.; Sales-Neto, V. N.

doi:10.1159/000200239

Cited by 15 publications

(14 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An experimental model similar to that of the digestive form of Chagas' disease, which also involves denervation of the myenteric plexus is chemical ablation using serosal surface application of benzalkonium chloride which induces disturbances of motility and absorption. However, there is little information about epithelial morphological changes and thickening of smooth muscle in the colon [14,26]. Thus, the present study was designed to investigate smooth muscle thickening and epithelial cell proliferation in the descending colon after different periods of myenteric neural denervation.…”

Section: Introductionmentioning

confidence: 98%

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

et al. 1997

View full text Add to dashboard Cite

The effects of myenteric neuronal denervation on smooth muscle thickening and epithelial cell proliferation were studied in the descending colon of rats treated by serosal application of 2 mM benzalkonium chloride (BAC) for 30 min. Control animals were treated with saline (0.9% NaCl). The animals were divided into six groups of 13 animals each and killed 10, 45 and 120 days after BAC treatment. A significant reduction in neuron number was observed in the myenteric plexus of animals treated with BAC, as well as smooth muscle thickening and an increase in crypt cell population, crypt cell production per crypt and a decrease in cell cycle time. These findings permit us to conclude that a relationship may exist between the increase of epithelial cell proliferation, smooth muscle thickening and myenteric neuron denervation in the descending colon caused by BAC, the latter probably playing an important role in the integration of the other two.

show abstract

Section: Introductionmentioning

confidence: 98%

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Chagas' disease is endemic in many countries of Latin America and is characterized by intrinsic visceral denervation, which leads to visceromegalies, mainly in the intestine [7]. There is an experimental model of intrinsic enteric denervation that resembles Chagas' disease [8], which is obtained by topical serosal application of benzalkonium chloride (BAC), a cationic surfactant agent that selectively destroys the myenteric neurones [9]. We have previously reported that ileal myenteric denervation by BAC leads to the development of asymptomatic megaileum in rats, with visceral dilatation and mucosal hyperplasia [10].…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Myenteric Denervation: A New Model to Increase the Intestinal Absorptive Surface in Short-Bowel Syndrome

Garcia

Kawasaky

Silva

et al. 1999

Journal of Surgical Research

View full text Add to dashboard Cite

“…Subsequently, the hearts and livers were excised and weighed and fragments of right inferior lobe of the lung were collected and sent for routine histological examinations. A series of 15 cross-sectional sections of the atria of each animal were assessed to determine the number of heart intrinsic neurons, according to the method described by OLIVEIRA et al [8].…”

Section: Histopathologic Studymentioning

confidence: 99%

“…Thus, there are no descriptions in the literature of a specific experimental model of denervation of the CINS, which preserves the extrinsic system. Benzalkonium chlorate (BC) is a cationic surfactant that, when applied topically to the digestive tract, causes denervation of intrinsic neurons, causing significant alterations to the physiology and morphology of this organ [7,8]. As the cardiac neurons are located in the epicardium [9], by analogy, the intra-pericardial administration of BC should destroy them, producing an experimental model of denervation.…”

Section: Introductionmentioning

confidence: 99%

Um modelo experimental de ablação do Sistema Nervoso Intrínseco Cardíaco reduz a contratilidade do coração de ratos

Filho¹,

Nakamura²,

Faria³

et al. 2004

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

Introduction: The function of the Intrinsic Cardiac Nervous System is largely unknown, as is its role in heart disease. In the digestive system, a topic application of Benzalkonium chloride leads to intrinsic denervation of the viscera. Thus, our aim was to obtain an experimental model of cardiac intrinsic denervation by the application of Benzalkonium chloride on the heart. Method: Thirty male Wistar rats received intrapericardial injections of 0.3% Benzalkonium chloride (BC animals) and thirty similar animals received saline (C animals). After 15 days the animals were divided in three groups, with 10 CBtreated and 10 saline-treated animals in each. Group I was submitted to radiological and morphologic studies. The cardiac shadow area (CSA) and cardiothoracic index were calculated in roentgenograms with a semi-automatic image analysis system (MINI-MOP). The day after the animals were weighed and sacrificed with heart, liver and lung collected for histopathologic analysis. The animals of Group II were submitted to a hemodynamic study. Measurements of blood pressure, heart rate and cardiac output were performed using the Cardiomax II thermodilution system and a Thermistor sensor. With the animals of the Group III, the integrity of the extrinsic parasympathetic cardiac innervation was examined by measuring heart rate response to electrical stimulation of the right vagus. Electrical activity was assessed by ECG.Results: CB animals presented increases in cardiothoracic index, CSA, body and liver weight. In these animals the histopathologic analysis showed passive chronic congestion and reduction of the number of atrial neurons. In the hemodynamic study, total peripheral resistance and heart rate were similar in both groups, but blood pressure and cardiac index were reduced in the CB group. The vagal stimulation and ECG were similar in both groups.Conclusion: Intrinsic Cardiac Nervous System denervation caused dilated cardiopathy in rats with left and right heart failure. The etiology of some dilated cardiopathies in human is largely unknown. This unpublished experimental model should provide future studies with the objective of elucidating the relationship between neuronal injures and heart disease.

show abstract

Megacolon in Rats

Cited by 15 publications

References 10 publications

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

The relationship between myenteric neuronal denervation, smooth muscle thickening and epithelial cell proliferation in the rat colon

Intrinsic Myenteric Denervation: A New Model to Increase the Intestinal Absorptive Surface in Short-Bowel Syndrome

Um modelo experimental de ablação do Sistema Nervoso Intrínseco Cardíaco reduz a contratilidade do coração de ratos

Contact Info

Product

Resources

About