Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting

Abstract

The clonal cherry rootstock CAB-6P (Prunus cerasus L.) is a commercial cherry rootstock with limited practical use compared to Prunus avium L. and Prunus mahaleb (Dimassi-Theriou, Therios 2006). In general, sour cherry as a rootstock is reported to be winter-hardy, tolerant to wet soils but inconsistent compatibility with some sweet cherry cultivars. CAB-6P is a rootstock suitable for sweet and sour cherry varieties. Trees in the nursery are moderately vigorous and have an intermediate growth habit. The root system is moderately developed with good anchorage in the ground; tendency to suckering. It adapts well to heavy soils with low permeability, it is resistant to calcareous soils (9% active lime) as well as to nematodes and it is suitable for replanting.Gisela 6 is a hybrid of Prunus cerasus × Prunus canescens and used as a semi-dwarf rootstock for all kinds of sweet cherry. It is also satisfactory in a wide range of soils, especially heavy soils (Andersen et al. 1999). The average and slow growth of this rootstock has a great value for the establishment and development of modern intensive cherry orchards. Trees grafted on this rootstock do not have any suckering problems (Long, Kaiser 2010). Producing Gisela 6 rootstock in large scale using conventional methods such as cuttings and layering in order to meet the growing internal demand The effects of two strength media (full and half ) in inorganics and five polyvinylpyrrolidone (PVP) concentrations combined with 1 mg/l indole-3-butyric acid on the in vitro rooting of the CAB-6P, Gisela 6 and M × M 14 cherry rootstocks were investigated. For the CAB-6P, root number (5.80) was greatest by adding 5 g/l PVP to the half strength medium and 1 g/l PVP resulted in 80% rooting percentage. For Gisela 6, 1 g/l PVP in half strength medium led to the highest root number (6.30) as well as the highest rooting percentage (90.91%). On the contrary, root length was maximum (36.17 mm) in the full strength medium without adding PVP. In M × M 14, root number was maximum (8.33) in the PVP-free full strength medium, rooting percentage was 100% by adding 5 g/l PVP to either full or half strength medium and root length was greatest by incorporating 1 or 2.5 g/l PVP into the half strength medium. PVP seems to be a promising rooting agent in tissue culture systems.

OPTIMASI MEDIUM PERBANYAKAN IN VITRO TUNAS TALAS KALIURANG (Colocasia esculenta L.) DIPLOID DAN TETRAPLOID

“…Umbi talas bernilai gizi cukup tinggi, di antaranya mengandung karbohidrat, protein, vitamin B-kompleks, vitamin A, vitamin C, dan serat (Darkwa and Darkwa 2013, Sepulveda-Nieto et al 2017, Wada et al 2017. Talas sangat beragam di Indonesia; terdapat sekitar 180 kultivar, salah satunya adalah talas kultivar Kaliurang (Prana and Kuswara 2002). Talas Kaliurang telah lama dibudidayakan dan dikonsumsi oleh masyarakat di daerah Kaliurang, Yogyakarta, karena rasanya enak dan pulen, tanamannya toleran terhadap penyakit, dan tahan terhadap serangan hama.…”

“…Talas Kaliurang telah lama dibudidayakan dan dikonsumsi oleh masyarakat di daerah Kaliurang, Yogyakarta, karena rasanya enak dan pulen, tanamannya toleran terhadap penyakit, dan tahan terhadap serangan hama. Namun pengembangan talas Kaliurang belum pernah ditangani secara serius (Prana and Kuswara 2002). Hal itu menjadikan talas memiliki peluang besar untuk dikembangkan dalam upaya diversifikasi pangan.…”

“…Fase ini berhasil menginduksi akar 100% pada tunas yang sebelumnya belum berakar, dari semua media perlakuan perbanyakan in vitro tunas (Gambar 3). Media ½ MS mampu menghasilkan jumlah dan panjang akar yang lebih baik dibandingkan dengan media MS konsentrasi penuh (Sarropoulou et al 2015).…”

Impact of green initial cuttings of vegetative-active grafting points of cherry on rooting and its period, rods, and growth controlling plant substance concentration

“…The sensitivity of a number of fruit plants that are not prone to vegetative propagation to treatment with growth-regulating substances has been proven in the experiments of many scientists in recent years [8]. Under the influence of these substances is observed hydrolysis of starch, as well as acceleration of the arrival of sugars and nitrogenous substances from the leaves to the lower parts of the cuttings [9].…”

“…In addition, there are reports that mival, cherkas, germatranol and other substances have also been tested in fruit plants and good results have been obtained. The peculiarities of vegetatively propagating clone grafts of cherries from green cuttings have been very well illuminated in several studies [7][8][9][10][11].…”