Medium Optimization for the Production of Probiotic Lactobacillus acidophilus A12 Using Response Surface Methodology

Lee, Na Kyoung; Park, Yeo Lang; Choe, Ga Jin; Chang, Hyo Ihl; Paik, Hyun Dong

doi:10.5851/kosfa.2010.30.3.359

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Furthermore, 0.15% magnesium sulfate was foremost important salt that could stimulate GSH production and cell growth. Lee et al (2010) reported similar effect for magnesium sulfate on L. acidophilus A12. In the present work, all the above media components were already included in MRS in addition to other ingredients that are sufficient to enhance the cell density and GSH productivity.…”

Section: Resultsmentioning

confidence: 61%

Optimization of Reduced Glutathione Production by a Lactobacillus plantarum Isolate Using Plackett–Burman and Box–Behnken Designs

2017

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In this work, we aim to optimize the production of reduced glutathione (GSH) synthesized intracellularly by a food-grade microorganism through a statistical approach. Using a colorimetric method, 25 Lactobacillus plantarum isolates were screened in an attempt to find a GSH-producing strain. It was found that 36% of the tested isolates showed positive result. Isolate (L7) was found to produce 152.61 μM glutathione per gram which was the highest amount produced intracellularly. Accordingly, the later isolate was selected for the optimization process using Plackett–Burman and Box–Behnken designs. Temperature, amino acids, and urea were found to be the most significant independent variables. Following data analysis, the composition of the optimized medium was De Man-Sharp-Rogosa broth as a basal medium supplemented with NaCl (5%), H2O2 (0.05%), sodium dodecyl sulfate (0.05%), amino acids (0.0281%), and urea (0.192%). The pH of the medium was adjusted to 8 and incubated for 24 h at 40°C. The GSH amount was increased by 10-fold (851%) using the optimized medium. Hence, our optimization design estimated the biotechnological potential of L. plantarum (L7) for the production of GSH in the industry.

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Section: Resultsmentioning

confidence: 61%

Optimization of Reduced Glutathione Production by a Lactobacillus plantarum Isolate Using Plackett–Burman and Box–Behnken Designs

2017

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“…After optimization, the optimum medium components for biomass production were found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced (Lee et al, 2010) . The growth kinetics of probiotic Lactobacillus strains in the alternative, costefficient semi-solid fermentation medium was studied by Śliżewska and Chlebicz-Wójcik (2020).…”

Section: Resultsmentioning

confidence: 99%

Pilot-Plant Scale Biomass Production by Lactobacillus Rhamnosus Gg Atcc 53103: A Comparison Between Batch and Fed-Batch Fermentation

Armand

Ghasemi

Fazeli³

et al. 2023

J microb biotech food sci

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Probiotics such as Lactobacilli are important in improving normal intestinal flora and hindering the growth of harmful bacteria in the digestive system. Given the above reasons, the industrial production of probiotics and the use of high-yield strains is of great importance. The present study compares the biomass production by Lactobacillus rhamnosus GG ATCC 53103 in batch and fed-batch cultures conditions at a pilot plant scale. An optimized medium containing the following compounds (g/L): glucose 112.50, sugar beet molasses 56.25, casein 18.75, yeast extract 18.75, K2HPO4 13.13, Tween 80 1.88, MgSO4.7H2O 0.3750, MnSO4. 4H2O 0.0750, CaCl2. 2H2O 0.1875 and Simethicone 1.25 was used for biomass production. During the fermentation process, culture conditions such as pH, temperature, and oxygen concentration were monitored using process analytical technology (PAT). Based on the obtained results, the maximum biomass production in the batch condition in the first 20 hours of culture in the optimized medium was about 68.14 g/L. After three stages of fed-batch culture, the biomass production by L. rhamnosus GG ATCC 53103 reached 93.5 g/L at 37°C with agitation and aeration rates of 100 rev/min and 300 VV-1min-1, respectively. Therefore, biomass production increased about 2.67-fold more than the basal medium.

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Optimization of Media Composition for Maximum Growth of Probiotic Lactobacillus fermentum NBC-08 Using Response Surface Methodology

KAVAK¹,

Selen

TAMTÜRK³

2022

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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In this study, it was aimed to determine the medium where Lactobacillus fermentum microorganisms with probiotic properties grow most efficiently by using Response Surface Method (RSM). Studies have been conducted on a 500 ml erlenmeyer scale, the medium optimization of the Lactobacillus fermentum strain was carried out according to the Central Composite Design (CCD) included in RSM. The effects of glucose, yeast extract, inorganic salts, and Tween 80 were investigated on the growth rate of the L. fermentum NBC-08 strain. Samples were taken at regular intervals from the erlenmeyer flask and the number of viable cells was measured by planting them in petri agar medium. In the study, the number of viable cells log10 (cfu/ml) was chosen as the response variable. As a result of the study, it was concluded that glucose and yeast extract are absolutely essential components in the medium. The optimum medium composition was found as 96.06 g/L glucose, 40.76 g L-1 yeast extract, 19.43 g L-1 inorganic salts, and 11.01 ml/L Tween 80. The production of the maximum L. fermentum strain was determined as 10.75 log10 (cfu ml-1). It is predicted that this study will make positive contributions to the fermentation conditions and medium optimization studies for production of lactic acid bacteria

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Medium Optimization for the Production of Probiotic Lactobacillus acidophilus A12 Using Response Surface Methodology

Cited by 4 publications

References 19 publications

Optimization of Reduced Glutathione Production by a Lactobacillus plantarum Isolate Using Plackett–Burman and Box–Behnken Designs

Optimization of Reduced Glutathione Production by a Lactobacillus plantarum Isolate Using Plackett–Burman and Box–Behnken Designs

Pilot-Plant Scale Biomass Production by Lactobacillus Rhamnosus Gg Atcc 53103: A Comparison Between Batch and Fed-Batch Fermentation

Optimization of Media Composition for Maximum Growth of Probiotic Lactobacillus fermentum NBC-08 Using Response Surface Methodology

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