Medium Optimization for Production of Beauveriabassiana BNBCRC Spores from Biohydrogen Effluent of Palm Oil Mill Using Taguchi Design

Petlamul, Wanida; Prasertsan, Poonsuk

doi:10.7763/ijbbb.2014.v4.320

Cited by 1 publication

(1 citation statement)

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“…The method also accounts multiple interaction possibilities between the parameters which is significant in industrial applications. Unlike the past decade, many works were currently relying on taguchi methods for culture parameter optimization [ 46 ], process optimization [ 47 , 48 ], medium optimization [ 49 , 50 ] and overall yield of enzyme production [ 51 ]. In this optimization process the most influencing factors affecting laccase production were found to be cupric sulphate, followed by substrate length and inoculum concentration.…”

Section: Resultsmentioning

confidence: 99%

Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

et al. 2017

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BackgroundFungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production.ResultsProduction of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications.ConclusionStudy optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.

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