Medium Optimization and Proteome Analysis of Protease Production by Janthinobacterium sp.

Kim, Hyun-do; Choi, Jong‐il

doi:10.1007/s12257-020-0110-x

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Table 3 shows the ANOVA results for the quadratic model of BC production. The F -value, which indicates the accuracy of the model [ 28 ], for the predictive model was 25.28. A value of p < 0.05 was considered statistically significant [ 29 ], and the results of our predictive model were demonstrated to be statistically significant ( p < 0.0001).…”

Section: Resultsmentioning

confidence: 99%

Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Son

Lee

et al. 2022

IJERPH

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Biorefineries are attracting attention as an alternative to the petroleum industry to reduce carbon emissions and achieve sustainable development. In particular, because forests play an important role in potentially reducing greenhouse gas emissions to net zero, alternatives to cellulose produced by plants are required. Bacterial cellulose (BC) can prevent deforestation and has a high potential for use as a biomaterial in various industries such as food, cosmetics, and pharmaceuticals. This study aimed to improve BC production from lignocellulose, a sustainable feedstock, and to optimize the culture conditions for Gluconacetobacter xylinus using Miscanthus hydrolysates as a medium. The productivity of BC was improved using statistical optimization of the major culture parameters which were as follows: temperature, 29 °C; initial pH, 5.1; and sodium alginate concentration, 0.09% (w/v). The predicted and actual values of BC production in the optimal conditions were 14.07 g/L and 14.88 g/L, respectively, confirming that our prediction model was statistically significant. Additionally, BC production using Miscanthus hydrolysates was 1.12-fold higher than in the control group (commercial glucose). Our result indicate that lignocellulose can be used in the BC production processes in the near future.

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Section: Resultsmentioning

confidence: 99%

Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Son

Lee

et al. 2022

IJERPH

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“…The mean squares were computed by dividing the sum of squares by the degrees of freedom [ 31 ]. The accuracy of models was described by the F-value [ 32 ]. F-values of each model were found to be 10.99 for GC and 9.42 for ED.…”

Section: Resultsmentioning

confidence: 99%

Improvement of Enzymatic Glucose Conversion from Chestnut Shells through Optimization of KOH Pretreatment

Lee

et al. 2021

IJERPH

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Worldwide, about one-third of food produced for human consumption is wasted, which includes byproducts from food processing, with a significant portion of the waste still being landfilled. The aim of this study is to convert chestnut shells (CNSs) from food processing into a valuable resource through bioprocesses. Currently, one of the highest barriers to bioprocess commercialization is low conversion of sugar from biomass, and KOH pretreatment was suggested to improve enzymatic digestibility (ED) of CNS. KOH concentration of 3% (w/w) was determined as a suitable pretreatment solution by a fundamental experiment. The reaction factors including temperature, time and solid/liquid (S/L) ratio were optimized (77.1 g/L CNS loading at 75 °C for 2.8 h) by response surface methodology (RSM). In the statistical model, temperature and time showed a relatively significant effect on the glucan content (GC) and ED, but S/L ratio was not. GC and ED of the untreated CNS were 45.1% and 12.7%, respectively. On the other hand, GC and ED of pretreated CNS were 83.2% and 48.4%, respectively, and which were significantly improved by about 1.8-fold and 3.8-fold compared to the control group. The improved ED through the optimization is expected to contribute to increasing the value of byproducts generated in food processing.

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Biotechnological potential of cold-adaptive extremozymes

Sarsan,

Rodhe,

Roy

et al. 2024

Microbial Essentialism

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Medium Optimization and Proteome Analysis of Protease Production by Janthinobacterium sp.

Cited by 4 publications

References 26 publications

Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Improvement of Enzymatic Glucose Conversion from Chestnut Shells through Optimization of KOH Pretreatment

Biotechnological potential of cold-adaptive extremozymes

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