Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Son, Jemin; Lee, Kang Hyun; Lee, Taek; Kim, Hyun Soo; Shin, Weon Ho; Oh, Jong‐Min; Koo, Sang‐Mo; Yu, Byung Jo; Yoo, Hah Young; Park, Chulhwan

doi:10.3390/ijerph19020866

Cited by 22 publications

(10 citation statements)

References 37 publications

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“…The studies on BC as a precursor of CNs are associated with excellent and unique nanoscale properties [ 30 , 33 , 34 , 35 , 36 ], with hydrolyzates from pretreated lignocellulose being examined as the nutrient medium [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. There are examples of hydrolyzates derived from Miscanthus [ 32 , 45 ]. Additionally, there is a need to upscale the biosynthesis process to ensure sufficient amounts of the precursor for promising CNs [ 46 ], but studies of this kind are scarce.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Production of Cellulose Nitrates and Bacterial Cellulose from Lignocellulose of Energy Crop

Kashcheyeva,

Korchagina,

Gismatulina

et al. 2023

Polymers

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This study is focused on exploring the feasibility of simultaneously producing the two products, cellulose nitrates (CNs) and bacterial cellulose (BC), from Miscanthus × giganteus. The starting cellulose for them was isolated by successive treatments of the feedstock with HNO3 and NaOH solutions. The cellulose was subjected to enzymatic hydrolysis for 2, 8, and 24 h. The cellulose samples after the hydrolysis were distinct in structure from the starting sample (degree of polymerization (DP) 1770, degree of crystallinity (DC) 64%) and between each other (DP 1510–1760, DC 72–75%). The nitration showed that these samples and the starting cellulose could successfully be nitrated to furnish acetone-soluble CNs. Extending the hydrolysis time from 2 h to 24 h led to an enhanced yield of CNs from 116 to 131%, with the nitrogen content and the viscosity of the CN samples increasing from 11.35 to 11.83% and from 94 to 119 mPa·s, respectively. The SEM analysis demonstrated that CNs retained the fiber shape. The IR spectroscopy confirmed that the synthesized material was specifically CNs, as evidenced by the characteristic frequencies of 1657–1659, 1277, 832–833, 747, and 688–690 cm−1. Nutrient media derived from the hydrolyzates obtained in 8 h and 24 h were of good quality for the synthesis of BC, with yields of 11.1% and 9.6%, respectively. The BC samples had a reticulate structure made of interlaced microfibrils with 65 and 81 nm widths and DPs of 2100 and 2300, respectively. It is for the first time that such an approach for the simultaneous production of CNs and BC has been employed.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Production of Cellulose Nitrates and Bacterial Cellulose from Lignocellulose of Energy Crop

Kashcheyeva,

Korchagina,

Gismatulina

et al. 2023

Polymers

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“…, kiwifruit peel [ 32 ], citrus peel [ 33 ], pineapple waste [ 34 ], and wine pomace [ 35 ]) as well as lignocellulosic biomass ( e.g. , Miscanthus [ 36 ], sweet sorghum [ 37 ], and pine tree [ 15 ]) have been actively studied and reported as a potential feedstock for BC production. Similarly, we attempted to valorize CS through microbial conversion to BC, and the results were promising.…”

Section: Resultsmentioning

confidence: 99%

Microbial Production of Bacterial Cellulose Using Chestnut Shell Hydrolysates by Gluconacetobacter xylinus ATCC 53524

Lee

Kim

et al. 2022

J. Microbiol. Biotechnol.

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Bacterial cellulose (BC) is gaining attention as a carbon-neutral alternative to plant cellulose, and as a means to prevent deforestation and achieve a carbon-neutral society. However, the high cost of fermentation media for BC production is a barrier to its industrialization. In this study, chestnut shell (CS) hydrolysates were used as a carbon source for the BC-producing bacteria strain, Gluconacetobacter xylinus ATCC 53524. To evaluate the suitability of the CS hydrolysates, major inhibitors in the hydrolysates were analyzed, and BC production was profiled during fermentation. CS hydrolysates (40 g glucose/l) contained 1.9 g/l acetic acid when applied directly to the main medium. As a result, the BC concentration at 96 h using the control group and CS hydrolysates was 12.5 g/l and 16.7 g/l, respectively (1.3-fold improved). In addition, the surface morphology of BC derived from CS hydrolysates revealed more densely packed nanofibrils than the control group. In the microbial BC production using CS, the hydrolysate had no inhibitory effect during fermentation, suggesting it is a suitable feedstock for a sustainable and eco-friendly biorefinery. To the best of our knowledge, this is the first study to valorize CS by utilizing it in BC production.

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“…Similar to the relationship of temperature-dependent ATP expenditure and M13 phage production, other major parameters for phage production such as pH and ionic condition of medium could also be interpreted in the same manner. M13 phage assembly/extrusion is considered as a significant process for phage yield and is affected by many parameters such as pH and ionic environment, despite the relatively lower ATP expenditure proportion compared to phage protein synthesis [20,48,49]. The gp1/gp11 complex expressed by M13 phage is used for phage assembly/extrusion as an ATPase for ATP hydrolysis.…”

Section: Atp Expenditure Analysis and Comparison With Residual Energy...mentioning

confidence: 99%

Investigation of the Relation between Temperature and M13 Phage Production via ATP Expenditure

et al. 2022

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M13 bacteriophage is a promising biomolecule capable of various bionano and material science applications. The biomaterial can self-assemble into matrices to fabricate bioscaffolds using high phage concentration and high phage purity. Previous studies aimed to acquire these conditions in large-scale phage production and have identified the optimal culture temperature range at 28–31 °C. However, explanations as to why this temperature range was optimal for phage production is absent from the work. Therefore, in this study, we identified the relation between culture temperature and M13 phage production using ATP expenditure calculations to comprehend the high yield phage production at the optimal temperature range. We extended a coarse-grained model for the evaluation of phage protein and ribosomal protein synthesis with the premise that phage proteins (a ribosomal protein) are translated by bacterial ribosomes in E. coli through expenditure of ATP energy. By comparing the ATP energy for ribosomal protein synthesis estimated using the coarse-grained model and the experimentally calculated ATP expenditure for phage production, we interpreted the high phage yield at the optimal temperature range and recognized ATP analysis as a reasonable method that can be used to evaluate other parameters for phage production optimization.

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Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

Cited by 22 publications

References 37 publications

Simultaneous Production of Cellulose Nitrates and Bacterial Cellulose from Lignocellulose of Energy Crop

Simultaneous Production of Cellulose Nitrates and Bacterial Cellulose from Lignocellulose of Energy Crop

Microbial Production of Bacterial Cellulose Using Chestnut Shell Hydrolysates by Gluconacetobacter xylinus ATCC 53524

Investigation of the Relation between Temperature and M13 Phage Production via ATP Expenditure

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