Medical gas plasma promotes blood coagulation via platelet activation

Bekeschus, Sander; Poschkamp, Broder; Linde, Julia van der

doi:10.1016/j.biomaterials.2020.120433

Cited by 20 publications

(10 citation statements)

References 71 publications

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“…These stages can be influenced by CAP and thus make a final contribution to improved wound healing. Several studies have suggested CAP technology to be a useful tool for promoting hemostasis [ 28 , 29 ]. Furthermore, improved proliferation and remodeling after CAP treatment were observed [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

et al. 2021

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Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (TmaxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased TmaxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.

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Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

et al. 2021

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“…There was no conclusion on overall healing responses. Interestingly, the gas plasma treatment led to rapid hemostasis in pig wounds, a finding that was recently confirmed quantitatively in mice [ 119 ] and mechanistically in human donor blood [ 120 , 121 ] using the kINPen argon plasma jet. In another study using this jet, superficial and deep skin wounds in live pigs were exposed to gas plasma [ 9 ].…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 92%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

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“…The generation of cold plasma has recently developed into an innovative and attractive research field, and its application is the subject of different industries, especially in biophysics, plasma science, and medicine [ 142 ]. Physical plasmas are used to modify biorelevant surfaces, for decontamination purposes, and during argon (hot) plasma coagulation to provide hemostasis via cauterization [ 143 , 144 ]. Moreover, cold physical plasmas at tissue-tolerable temperatures of around 40°C are used therapeutically and directly on the body surfaces due to their antibacterial, anti-inflammatory, and wound healing properties [ 145 ].…”

Section: Plasma Medicinementioning

confidence: 99%

H2A.X Phosphorylation in Oxidative Stress and Risk Assessment in Plasma Medicine

Schütz

Stope²,

Bekeschus

2021

Oxidative Medicine and Cellular Longevity

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At serine139-phosphorylated gamma histone H2A.X (γH2A.X) has been established over the decades as sensitive evidence of radiation-induced DNA damage, especially DNA double-strand breaks (DSBs) in radiation biology. Therefore, γH2A.X has been considered a suitable marker for biomedical applications and a general indicator of direct DNA damage with other therapeutic agents, such as cold physical plasma. Medical plasma technology generates a partially ionized gas releasing a plethora of reactive oxygen and nitrogen species (ROS) simultaneously that have been used for therapeutic purposes such as wound healing and cancer treatment. The quantification of γH2A.X as a surrogate parameter of direct DNA damage has often been used to assess genotoxicity in plasma-treated cells, whereas no sustainable mutagenic potential of the medical plasma treatment could be identified despite H2A.X phosphorylation. However, phosphorylated H2A.X occurs during apoptosis, which is associated with exposure to cold plasma and ROS. This review summarizes the current understanding of γH2A.X induction and function in oxidative stress in general and plasma medicine in particular. Due to the progress towards understanding the mechanisms of H2A.X phosphorylation in the absence of DSB and ROS, observations of γH2A.X in medical fields should be carefully interpreted.

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Medical gas plasma promotes blood coagulation via platelet activation

Cited by 20 publications

References 71 publications

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

H2A.X Phosphorylation in Oxidative Stress and Risk Assessment in Plasma Medicine

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