This paper first introduces the catalytic electrochemical biosensors, analyses their basic principle as well as the types and so on, and from the angle of experiment, this paper discusses the research and application of paperbased glucose electrochemical biosensors. Two application experiments, namely, the experiment of disposable capillary sampling biamperometry glucose biosensor and biamperometry analysis method on paper-based glucose biosensor according to the injecting process are conducted, and through the analysis and discussion of the experimental results, the final conclusions are reached.