Medaka dead end encodes a cytoplasmic protein and identifies embryonic and adult germ cells

Liu, Lixiu; Hóng, Ní; Xu, Hongyan; Li, Mingyou; Yan, Yan; Purwanti, Yovita Ida; Yi, Meisheng; Li, Zhendong; Wang, Li; Hong, Yunhan

doi:10.1016/j.gep.2009.06.008

Cited by 66 publications

(79 citation statements)

References 33 publications

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“…This procedure features multiple combinations between bright-field optics and three colors of fluorescent optics, allowing for precise comparisons of the relative signal intensity and distribution of different molecules. FISH in medaka provides detection sensitivity~100 times that of the chromogenic ISH (Liu et al, 2009;Xu et al, 2009). …”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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Germ cells make two major decisions when they move from an indeterminate state to their final stage of gamete production. One decision is sexual commitment for sperm or egg production, and the other is to maintain mitotic division or entry into meiosis. It is unclear whether the two decisions are made as a single event or separate events, because there has been no evidence for the presence of germ cell sex prior to meiosis. Here we report direct evidence in the fish rainbow trout that gonia have distinct sexuality. We show that dazl expression occurs in both male and female gonia but exhibits differential intracellular distribution. More strikingly, we show that boule is highly expressed in male gonia but absent in female gonia. Therefore, mitotic gonia possess sex, sperm/egg decision and mitosis/meiosis decision are two independent events, and sperm/egg decision precedes mitosis/meiosis decision in rainbow trout, making this organism a unique vertebrate model for mechanistic understanding of germ cell sex differentiation and relationship between the two decisions.

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Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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“…On the contrary, mouse dnd1 expression is restricted to germ cells of adult testis (Bhattacharya et al, 2007). The adult expression of medaka dnd occurs in germ cells of both sexes (Liu et al, 2009). This expression pattern was also found in turbot Scophthalmus maximus (Lin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Dead end (dnd) is a germ plasm component in vertebrates that is crucial for zebrafish PGC migration and survival (Weidinger et al, 2003). The gene has been subsequently isolated in diverse vertebrates, including mouse, Xenopus, chicken, and medaka (Youngren et al, 2005;Horvay et al, 2006;Aramaki et al, 2007;Liu et al, 2009). In mouse, mutations of the dnd gene were shown to cause germ cell loss and increase the incidence of testicular germ cell tumor (Youngren et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis

Yang

Yue

et al. 2015

Gene

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“…In one study, the knockdown of Dnd resulted in loss of germ cells without effects on the somatic cells in zebrafish (Weidinger et al 2003). The homologous genes of dnd have been identified in medaka (Oryzias latipes) (Liu et al 2009), loach (Misgurnus anguillicaudatus) (Fujimoto et al 2010), African clawed frog (Xenopus laevis) (Weidinger et al 2003;Horvay et al 2006), chicken (Gallus gallus) (Weidinger et al 2003;Aramaki et al 2009) and mouse (Mus musculus) (Weidinger et al 2003;Youngren et al 2005;Bhattacharya et al 2007). Loss of dnd also resulted in loss of germ cells in loach (Fujimoto et al 2010), frog (Horvay et al 2006) and mouse (Youngren et al 2005;Bhattacharya et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)

Duan

Feng

Chang

et al. 2015

Fish Physiol Biochem

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Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow.

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Medaka dead end encodes a cytoplasmic protein and identifies embryonic and adult germ cells

Cited by 66 publications

References 33 publications

Germ cell sex prior to meiosis in the rainbow trout

Germ cell sex prior to meiosis in the rainbow trout

Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis

Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)

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