Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)

Duan, Jundan; Feng, Gongqing; Chang, Pei Ching; Zhang, Xueyan; Zhou, Qingchun; Chen, Qi; Shen, Xie; Zhao, Haobin

doi:10.1007/s10695-015-0029-x

Cited by 11 publications

(5 citation statements)

References 35 publications

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“…However, its established role as an anchor for maternal RNAs in unfertilised eggs and polymerization of cortical microtubule arrays has been demonstrated in Xenopus (Mei et al, 2013) needs verification in teleost. The subsequent occurrence of dnd in the embryonic and postnatal germline of G. holbrooki is consistent with those reported for other vertebrates (Youngren et al, 2005;Aramaki et al, 2007) including teleosts (Su et al, 2014;Duan et al, 2015;Wargelius et al, 2016) maintaining germline identity by protecting transcripts from miRNAmediated decay (Ketting, 2007;Goudarzi et al, 2013), FIGURE 7 | Comparative PGC development in representative lecithotrophic and matrotrophic species with increasing level of maternal care. In zebrafish (Raz, 2003;Eno and Pelegri, 2013) and G. holbrooki, PGC markers are compartmentalised in four distinct cells (red dots) by cleavage (8-cell and Cl4, respectively).…”

Section: Pgc Migration Pattern Shares Features Of Both Egg Laying Tel...supporting

confidence: 85%

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

Razmi

Patil

2022

Front. Cell Dev. Biol.

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Metazoans exhibit two modes of primordial germ cell (PGC) specification that are interspersed across taxa. However, the evolutionary link between the two modes and the reproductive strategies of lecithotrophy and matrotrophy is poorly understood. As a first step to understand this, the spatio-temporal expression of teleostean germ plasm markers was investigated in Gambusia holbrooki, a poecilid with shared lecitho- and matrotrophy. A group of germ plasm components was detected in the ovum suggesting maternal inheritance mode of PGC specification. However, the strictly zygotic activation of dnd-β and nanos1 occurred relatively early, reminiscent of models with induction mode (e.g., mice). The PGC clustering, migration and colonisation patterns of G. holbrooki resembled those of zebrafish, medaka and mice at blastula, gastrula and somitogenesis, respectively—recapitulating features of advancing evolutionary nodes with progressive developmental stages. Moreover, the expression domains of PGC markers in G. holbrooki were either specific to teleost (vasa expression in developing PGCs), murine models (dnd spliced variants) or shared between the two taxa (germline and somatic expression of piwi and nanos1). Collectively, the results suggest that the reproductive developmental adaptations may reflect a transition from lecithotrophy to matrotrophy.

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Section: Pgc Migration Pattern Shares Features Of Both Egg Laying Tel...supporting

confidence: 85%

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

Razmi

Patil

2022

Front. Cell Dev. Biol.

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“…In some species, such as mouse and xenopus, dnd expression is sex-dependent [ 13 , 28 ]. However, in fish species, dnd is expressed in both sexes, as observed in this study [ 7 , 13 , 18 , 29 , 30 ]. The expression in both ovary and testis means psdnd has specific roles for oogenesis and spermatogenesis during sexual maturation in starry flounder.…”

Section: Discussionmentioning

confidence: 56%

“…Our WISH results revealed that the psdnd expression pattern was similar with the PGC localization pattern, suggesting that psdnd is a germplasm involved in PGC migration. Similar quantitative and spatial expression patterns occur for dnd in other vertebrate species, including olive flounder, turbot, and rare minnow [ 7 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 83%

Dead-End (dnd) Gene Cloning and Gonad-Specific Expression Pattern in Starry Flounder (Platichthys stellatus)

Yoon

Cho

Lee

et al. 2021

Animals

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dnd is a germline-specific maternal RNA expressed in various vertebrate classes, which encodes an RNA-binding protein that is essential for PGC migration. The purpose of this study is fundamental research about starry flounder dnd gene for germ cell marker development. In this study, we cloned and analyzed the expression levels of Platichthys stellatus dead end (psdnd) in various tissues and embryonic stages. The psdnd gene was isolated from starry flounder ovaries, cloned into a pGEM-t vector, and sequenced. Full-length of psdnd cDNA was 1495 bp long, encoding 395 amino acids. psdnd expression levels were investigated by real-time polymerase chain reaction (qRT-PCR) in various tissues and embryo developmental stages. psdnd transcripts were detected in the testes and ovaries, but not in somatic tissues. Embryonic psdnd expression levels were higher during early embryo development stages than during late embryogenesis; psdnd expression was highest at the 1 cell stage, then gradually decreased throughout the subsequent developmental stages. The spatial expression pattern was analyzed by whole-mount in situ hybridization (WISH). The psdnd transcripts migration pattern was similar with zebrafish (Danio rerio). Our results suggest that psdnd may function as a germ cell-specific marker.

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“…These mechanisms may involve the vertebrate-specific protein Dead end (Dnd), whose RNA is expressed in the germline of different vertebrate classes, as it has been shown to play a major role in germline development (Aramaki et al, 2007;Duan et al, 2015;Horvay et al, 2006;Wargelius et al, 2016;Weidinger et al, 2003;Youngren et al, 2005). Dnd was shown to protect specific mRNAs from microRNA (miRNA)-mediated inhibition within the germline (Kedde et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation

Groß-Thebing¹,

Yigit²,

Pfeiffer³

et al. 2017

Developmental Cell

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Maintaining cell fate relies on robust mechanisms that prevent the differentiation of specified cells into other cell types. This is especially critical during embryogenesis, when extensive cell proliferation, patterning, and migration events take place. Here we show that vertebrate primordial germ cells (PGCs) are protected from reprogramming into other cell types by the RNA-binding protein Dead end (Dnd). PGCs knocked down for Dnd lose their characteristic morphology and adopt various somatic cell fates. Concomitantly, they gain a gene expression profile reflecting differentiation into cells of different germ layers, in a process that we could direct by expression of specific cell-fate determinants. Importantly, we visualized these events within live zebrafish embryos, which provide temporal information regarding cell reprogramming. Our results shed light on the mechanisms controlling germ cell fate maintenance and are relevant for the formation of teratoma, a tumor class composed of cells from more than one germ layer.

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Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)

Cited by 11 publications

References 35 publications

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

Dead-End (dnd) Gene Cloning and Gonad-Specific Expression Pattern in Starry Flounder (Platichthys stellatus)

The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation

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