Meconial Methanobrevibacter smithii suggests intrauterine methanogen colonization in preterm neonates

Sérémé, Youssouf; Filleron, Anne; Corbeau, Pierre; Tran, Tú-Anh; Drancourt, Michel; Vitte, Joana; Grine, Ghiles

doi:10.1016/j.crmicr.2021.100034

Cited by 8 publications

(14 citation statements)

References 34 publications

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“…Intrauterine life is associated with low levels of maternal microbial translocation [ 52 ]. However, we have recently demonstrated the presence of the viable methanogenic Archaea Methanobrevibacter smithii in the meconium, suggesting intrauterine colonization of the fetus by this microorganism [ 26 ]. Here, we provided evidence for postnatal persistence of methanogenic Archaea in fecal samples and suggest a possible role in the orientation of intestinal immunity, supported by the negative association between Archaea abundance (inversely proportional to CT values) and the concentrations of EDN, IL-1β, and IL-6.…”

Section: Discussionmentioning

confidence: 99%

“…The amplification reaction was performed in a CFX96 thermocycler (BioRad, Marnes-la-Coquette, France) incorporating a protocol with a cycle of 50 °C for 2 min, followed by 39 cycles of 95 °C for 45 s, 95 °C for 5 s and finally 60 °C for 30 s. Samples with a CT < 40 were considered positive. Gene amplification and PCR sequencing were performed as previously described [ 25 , 26 , 32 , 33 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…We have shown that the neonatal gut is colonized by methanogenic Archaea from the first postnatal hours, possibly starting in utero [ 21 , 22 ]. Gut microbiome establishment is altered in preterm and low- birth-weight infants [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases

Sérémé

Michel

Mezouar

et al. 2022

JCM

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Background: Preterm birth is a major cause of morbidity and mortality in infants and children. Non-invasive methods for screening the neonatal immune status are lacking. Archaea, a prokaryotic life domain, comprise methanogenic species that are part of the neonatal human microbiota and contribute to early immune imprinting. However, they have not yet been characterized in preterm neonates. Objective: To characterize the gut immunological and methanogenic Archaeal (MA) signature in preterm neonates, using the presence or absence of atopic conditions at the age of one year as a clinical endpoint. Methods: Meconium and stool were collected from preterm neonates and used to develop a standardized stool preparation method for the assessment of mediators and cytokines and characterize the qPCR kinetics of gut MA. Analysis addressed the relationship between immunological biomarkers, Archaea abundance, and atopic disease at age one. Results: Immunoglobulin E, tryptase, calprotectin, EDN, cytokines, and MA were detectable in the meconium and later samples. Atopic conditions at age of one year were positively associated with neonatal EDN, IL-1β, IL-10, IL-6, and MA abundance. The latter was negatively associated with neonatal EDN, IL-1β, and IL-6. Conclusions: We report a non-invasive method for establishing a gut immunological and Archaeal signature in preterm neonates, predictive of atopic diseases at the age of one year.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases

Sérémé

Michel

Mezouar

et al. 2022

JCM

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“…Sequences were edited using ChromasPro software (ChromasPro 1.7, Technelysium Pty Ltd., Tewantin, Australia) as previously used Sereme et al, 2021;Sogodogo, Doumbo, et al, 2019;Sogodogo, Fellag, et al, 2019). Molecular phylogenetic and evolutionary analyses were conducted in MEGA7 as previously described (Kumar et al, 2016).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

“…Methanogens represent the archaea most present in the mammalian microbiota, especially in the human digestive microbiota where they account for 10% digestive tract of the anaerobic microorganisms (Dridi et al, 2011;Guindo, 2020;Nkamga et al, 2017). Methanogens are detected in humans from birth (Grine et al, 2017) and there is a diversification of methanogens in humans over the years: while only Methanobrevibacter smithii (M. smithii) has been detected and cultured in the neonates (Grine et al, 2017;Koenig et al, 2011;Mihajlovski et al, 2010;Odamaki et al, 2016;Palmer et al, 2007;Sereme et al, 2021), M. smithii, Methanosphaera stadtmanae (M. stadtmanae), Methanobrevibacter millerae (M.…”

Section: Introductionmentioning

confidence: 99%

Dairy products as sources of methanogens for humans

Drancourt

Grine

2021

Preprint

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Methanogens are detected in human gut from the first moments of life and there is a diversification of methanogens during infancy. However, the sources of acquisition of methanogens are not well elucidated. We therefore investigated 56 dairy products as potential sources of methanogens by applying molecular biology. In the presence of negative controls, we obtained an overall prevalence of methanogens in 85.7% (48/56) of samples by real-time PCR. Further PCR-sequencing identified 73.2% (41/56) of Methanobrevibacter smithii. We also found for the first time in dairy products 1.8% (1/56) of Methanobrevibacter oralis, 7.1% (4/56) of Methanobrevibacter millerae, 1.8% (1/56) of Methanobrevibacter ruminantium, 1.8% (1/56) of Methanocorpusculum sp. We observed a significant presence (p-value=0.001) of methanogens in fermented dairy products compared to unfermented dairy products. This study gives credit to the fact that dairy products could be considered as a source of methanogens for humans, especially for children.

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Rapid identification of clinically interesting methanogens using an improved MALDI-TOF-MS assay

Amir

Couderc

Drancourt

et al. 2022

Access Microbiology

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Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly being detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to identify broth-cultured methanogens by matrix-assisted laser desorption time-of-flight MS (MALDI-TOF-MS). A database incorporating 34 reference spectra derived from 16 methanogen reference strains representative of eight species supported further identification of 21 Methanobrevibacter smithii and 14 Methanobrevibacter oralis isolates broth-cultured from human stool and oral fluid, respectively, with scores >2. In addition, MALDI-TOF-MS differentiated five Methanobrevibacter smithii genotypes incorporated in the study. The data reported here found MALDI-TOF-MS as a first-line identification method for methanogens recovered from microbiota and clinical samples.

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Meconial Methanobrevibacter smithii suggests intrauterine methanogen colonization in preterm neonates

Cited by 8 publications

References 34 publications

A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases

A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases

Dairy products as sources of methanogens for humans

Rapid identification of clinically interesting methanogens using an improved MALDI-TOF-MS assay

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