Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Matsumura, Masanori; Nomoto, Mika; Itaya, Tomotaka; Aratani, Yuri; Iwamoto, Mizuki; Matsuura, Takakazu; Hayashi, Yuji; Mori, Tsuyoshi; Skelly, Michael; Yamamoto, Yoshiharu Y.; Kinoshita, Toshinori; Mori, Izumi C.; Suzuki, Takamasa; Betsuyaku, Shigeyuki; Spoel, Steven H.; Toyota, Masatsugu; Tada, Yutaka

doi:10.1038/s41467-022-28813-8

Cited by 51 publications

(50 citation statements)

References 79 publications

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“…A second independent transgenic line of pLipoP1∷NLS-3xmCitrine exhibited a similar expression pattern, but had more robust expression at 72h (Figure S6A). A previous transcriptional reporter CBP60gpro:EYFP-NLS has been characterized and this line exhibited similar baseline expression as well as expression in skirt cells (see below, Matsumura et al, 2022). Together, these data highlight activation of immune marker genes at early infection stages and downregulation at late stages, consistent with the pseudotime trajectory.…”

Section: Resultsmentioning

confidence: 99%

Single-cell profiling of complex plant responses toPseudomonas syringaeinfection

Zhu

Lolle

Tang

et al. 2022

Preprint

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Plant response to pathogen infection varies within a leaf, yet this heterogeneity is not well resolved. We exposed Arabidopsis to Pseudomonas syringae or mock treatment and profiled >11,000 individual cells using single-cell RNA sequencing. Integrative analysis of cell populations from both treatments identified distinct pathogen responsive cell clusters exhibiting transcriptional responses ranging from immunity to susceptibility. Pseudotime analyses through pathogen infection revealed a continuum of disease progression from an immune to susceptible state. Confocal imaging of promoter reporter lines for transcripts enriched in immune cell clusters expressed surrounding substomatal cavities colonized or in close proximity to bacterial colonies, suggesting cells within immune clusters represent sites of early pathogen invasion. Susceptibility clusters exhibited more general localization and were highly induced at later stages of infection. Overall, our work uncovers cellular heterogeneity within an infected leaf and provides unique insight into plant differential response to infection at a single-cell level.

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Section: Resultsmentioning

confidence: 99%

Single-cell profiling of complex plant responses toPseudomonas syringaeinfection

Zhu

Lolle

Tang

et al. 2022

Preprint

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“…(2022) are not surprising in the context of another recent study, which may help us to understand how this sensory system may have evolved. When the leaf trichome of non-carnivorous Arabidopsis thaliana transformed with the same Ca 2+ sensor (GCaMP3) was mechanically stimulated, a Ca 2+ wave was triggered, beginning at the trichome base before propagating away from the trichome as a concentric wave ( Matsumura et al., 2022 ). In the cells that received a Ca 2+ signal, the immune response was initiated in a phytohormone (e.g., JA)-dependent and independent manner.…”

Section: Main Textmentioning

confidence: 99%

“…These findings suggest that the prerequisite for the sensory system of Droseraceae was already present in non-carnivorous plants and that evolution only fine-tuned the process for botanical carnivory. Because the Ca 2+ wave in the Arabidopsis trichome does not enter neighboring trichomes ( Matsumura et al., 2022 ), an increase in tentacle density or a decrease in the resistance to Ca 2+ wave propagation may lead to coordination and complex trap behavior. Presumably, this hypothesis can be tested using mutants of transgenic GCaMP3 Arabidopsis with increased trichome density.…”

Section: Main Textmentioning

confidence: 99%

How the sensory system of carnivorous plants has evolved

Pavlovič

2022

Plant Communications

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“…Concerning the latter, the secretion of, for instance, phenolic substances (mainly by glandular trichomes) and the deployment of an initial physical barrier (mainly by non‐glandular trichomes) have been identified as pivotal strategies to prevent herbivore attack (Karabourniotis, Liakopoulos, Nikolopoulos, & Bresta, 2020). Moreover, non‐glandular trichomes of the model plant Arabidopsis thaliana were recently described as mechanosensors that initiate immune responses upon rainfall, to preempt attacks by rain‐dispersed fungal spores or bacteria (Matsumura et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Efficient Isolation and Purification of High‐Quality Arabidopsis thaliana Trichomes

2022

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Trichomes are fine outgrowths on the surface of aerial plant organs which play a role in protecting plants against water loss, UV radiation, and herbivore feeding. Throughout the years, trichomes have become a popular paradigm in biological research. For example, trichomes on rosette leaves of the reference plant Arabidopsis thaliana have been used as a model to investigate cell development, cell differentiation, and, more recently, cell wall biogenesis. State of the art ‐omics studies on specific cell types or tissues often require physical separation, enrichment, and purification. This, of course, also applies to leaf trichomes, and various methods have thus been proposed to separate trichomes and leaf tissue. Though most of these methods are indeed suitable for trichome isolation, they suffer in part from tedious operating procedures, low yield, poor sample purity, and reduced trichome integrity. We have thus revised a previously reported method for trichome isolation, and report here an efficient and scalable procedure for the isolation and gradient centrifugation–based purification of high‐quality A. thaliana trichomes. We describe the preparation of plant material and trichome release, which is based on prolonged gentle agitation of plant seedlings in the presence of a cation‐chelating agent that weakens trichome‐leaf interactions. We also outline the steps for the subsequent recovery and purification of the isolated crude trichome fraction, which is based on the use of discontinuous sucrose gradient centrifugation. In addition to A. thaliana, we have found that this procedure can be applied to release and enrich glandular and non‐glandular trichomes from various species, including Solanum lycopersicum and Nicotiana benthamiana. The resulting purified leaf trichomes can be subjected to different types of bioassays, including histochemistry, biochemical quantification of cell wall monosaccharides, and transcriptomics, as well as proteomic profiling. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Preparation of plant material for release and enrichment of A. thaliana trichomes Basic Protocol 2: Purification of A. thaliana trichomes by density gradient centrifugation

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Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Cited by 51 publications

References 79 publications

Single-cell profiling of complex plant responses toPseudomonas syringaeinfection

Single-cell profiling of complex plant responses toPseudomonas syringaeinfection

How the sensory system of carnivorous plants has evolved

Efficient Isolation and Purification of High‐Quality Arabidopsis thaliana Trichomes

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