Mechanobiological implications of age-related remodelling in the outer retina

Piskova, Teodora; Kozyrina, Aleksandra N.; Russo, Jacopo Di

doi:10.1016/j.bioadv.2023.213343

Cited by 6 publications

(2 citation statements)

References 120 publications

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“…In the present study, changes in the density of pigments of the RPE have been investigated as a measure of the presence of changes; RPE pigmentation changes are known as an indicator of AMD progression [15] . Our results showed that age had a significant correlation with the prevalence of RPE change; shortening of the RPE microvilli with age is probably due to thinning of this layer [26] . Although there are differences among studies regarding changes in the density of RPE cells [27] , Jonas et al [28] found that the number of the RPE cells decreased by about 0.3% per each one-year increase in age.…”

Section: Central Retinal Artery Occlusionmentioning

confidence: 51%

Prevalence of retinal pathologies in people over 60 years: the Tehran Geriatrics Eye Study

Mortazavi,

Jamali,

Hashemi

et al. 2024

Int J Ophthalmol

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AIM: To determine the prevalence of some retinal pathologies in people over 60y and their association with demographic and ocular factors. METHODS: A cross-sectional study was conducted in Tehran using multistage cluster sampling. After selecting subjects aged 60 and over, optometric, and ophthalmic examinations were done. For retinal examination, a 90 D lens was used and indirect ophthalmoscopy was performed after instilling tropicamide drops. Biometry was done using the IOL Master for all participants. RESULTS: Of 3791 people that were invited through cluster sampling, 3310 participated in the study (response rate=82%). The prevalence of retinal pigmented epithelium (RPE) change, drusen, geographic atrophy (GA), hypertensive retinopathy (HTR), nonproliferative diabetic retinopathy (NPDR), proliferative diabetic retinopathy (PDR), choroidal neovascularization (CNV), central retinal artery occlusion (CRAO), myopic retinopathy (MR), branch retinal vein occlusion (BRVO), and central retinal vein occlusion (CRVO) was 27.42%, 11.08%, 4.52%, 3.03%, 4.05%, 0.54%, 0.82%, 0.39%, 0.20%, 0.49%, and 0.19%, respectively. After removing the effect of age, the odds of NPDR were 1.68 times higher in women compared to men (P=0.014). After removing the effect of sex, the odds of drusen, RPE change, GA, CNV, BRVO, and CRVO increased with age. CONCLUSION: There is a higher prevalence of RPE change, drusen, GA, CNV and a lower prevalence of MR and CRAO in the elderly population of Tehran aged over 60y compared to global average values. Considering the correlation of most of the diseases with age and their effects on vision, attention should be paid to these diseases and the related screening programs to prevent vision impairment.

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Section: Central Retinal Artery Occlusionmentioning

confidence: 51%

Prevalence of retinal pathologies in people over 60 years: the Tehran Geriatrics Eye Study

Mortazavi,

Jamali,

Hashemi

et al. 2024

Int J Ophthalmol

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“…Previous studies have examined RPE cell morphometrics in the aging C57BL/6J mouse and found that there were regional differences in RPE cell morphology [ (23,55,71) . Additionally, these studies highlight the degree of stress exerted at the cell borders contribute to regional heterogeneity in RPE structures with central and midperipheral RPE cells manifesting morphological and functional damage markers first [ (16,(77)(78)(79)(80)(81)66)]. Considering the degree of heterogeneity that the RPE can display, we performed a regional analysis of cytoplasmic alpha catenin expression, cell area, and eccentricity to look for damage indicators.…”

Section: Discussionmentioning

confidence: 99%

Age-Related RPE changes in Wildtype C57BL/6J Mice between 2 and 32 Months

Shelton,

Gefke,

Summers

et al. 2024

Preprint

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Purpose: This study systematically evaluates age-related changes in RPE cell structure and function using a morphometric approach. We aim to better capture nuanced predictive changes in cell heterogeneity that reflect loss of RPE integrity during normal aging. Using C57BL6/J mice ranging from P60-P730, we sought to evaluate how regional changes in RPE shape reflect incremental losses in RPE cell function with advancing age. We hypothesize that tracking global morphological changes in RPE is predictive of functional defects over time. Methods: We tested three groups of C57BL/6J mice (young: P60-180; Middle-aged: P365-729; aged: 730+) for function and structural defects using electroretinograms, immunofluorescence, and phagocytosis assays. Results: The largest changes in RPE morphology were evident between the young and aged groups, while the middle-aged group exhibited smaller but notable region-specific differences. We observed a 1.9-fold increase in cytoplasmic alpha-catenin expression specifically in the central-medial region of the eye between the young and aged group. There was an 8-fold increase in subretinal, IBA-1-positive immune cell recruitment and a significant decrease in visual function in aged mice compared to young mice. Functional defects in the RPE are corroborated by changes in RPE phagocytotic capacity. Conclusions: The marked increase of cytoplasmic alpha-catenin expression and subretinal immune cell deposition, and decreased visual output coincide with regional changes in RPE cell morphometrics when stratified by age. These cumulative changes in the RPE morphology showed predictive, regional patterns of stress associated with loss of RPE integrity.

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