Mechanistic Insight into Phenolic Compounds Toxicity and State-of-the-art Strategies for Enhancing the Tolerance of Escherichia coli to Phenolic Compounds

Liu, Lina; Ma, Xiaolong; Bilal, Muhammad; Wei, Luqing; Tang, Shijie; Luo, Hongzhen; Zhao, Yuping; Duan, Xuguo

doi:10.1007/s12257-022-0019-7

Cited by 3 publications

(2 citation statements)

References 98 publications

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“…Figure 3 f–i show that the survival rate of Δ csg D was significantly lower than that of the parent strain under acid, alkali and osmotic pressure ( p < 0.05). Under oxidative stress, there was no significant difference in the survival rate between the two strains ( p > 0.05) because the original strain E. coli BL21(DE3) had weak tolerance and defense mechanisms against oxidative stress [ 9 , 30 ]. Although the survival rate of Δ csg D in a hydrogen peroxide environment was only 2.60 ± 0.14 × 10 5 CFU/mL, there was no significant difference from the parent strain ( p > 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, the mature genetic engineering tools and technology of E. coli make it relatively simple and efficient to carry out genetic transformation. However, engineered E. coli is more vulnerable to multiple environmental stresses in application, especially in reaction conditions such as acids, alkalis, organic solvents and metal ions [ 8 , 9 ]. It is worth noting that biofilms play an important role in increasing the survivability of E. coli in stressful environments.…”

Section: Introductionmentioning

confidence: 99%

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The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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The high cell density, immobilization and stability of biofilms are ideal characteristics for bacteria in resisting antibiotic therapy. CsgD is a transcription activating factor that regulates the synthesis of curly fimbriae and cellulose in Escherichia coli, thereby enhancing bacterial adhesion and promoting biofilm formation. To investigate the role of CsgD in biofilm formation and stress resistance in bacteria, the csgD deletion mutant ΔcsgD was successfully constructed from the engineered strain E. coli BL21(DE3) using the CRISPR/Cas9 gene-editing system. The results demonstrated that the biofilm of ΔcsgD decreased by 70.07% (p < 0.05). Additionally, the mobility and adhesion of ΔcsgD were inhibited due to the decrease in curly fimbriae and extracellular polymeric substances. Furthermore, ΔcsgD exhibited a significantly decreased resistance to acid, alkali and osmotic stress conditions (p < 0.05). RNA-Seq results revealed 491 differentially expressed genes between the parent strain and ΔcsgD, with enrichment primarily observed in metabolism-related processes as well as cell membrane structure and catalytic activity categories. Moreover, CsgD influenced the expression of biofilm and stress response genes pgaA, motB, fimA, fimC, iraP, ompA, osmC, sufE and elaB, indicating that the CsgD participated in the resistance of E. coli by regulating the expression of biofilm and stress response. In brief, the transcription factor CsgD plays a key role in the stress resistance of E. coli, and is a potential target for treating and controlling biofilm.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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Antimicrobial and antibiofilm activities of chromone derivatives against uropathogenic Escherichia coli

Boya,

Lee,

Lee

2024

Microbiological Research

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An Overview of the Role of Membrane Proteins in Microbial Solvents Tolerance

Mei

Ren

2023

PPL

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Background: Solvent tolerance is a desired feature of microorganisms for their application in biotechnology. Organic solvent-tolerant microorganisms are able to thrive in the presence of organic solvents. Several mechanisms have been proposed to elucidate their intrinsic tolerance to organic solvents. Objective: The present review aims to summarize the state of the art of the roles of membrane proteins in microbial organic solvent tolerance. Strategies and challenges for improving the protective function of membrane proteins in organic solvent stress are also proposed. Results: Membrane proteins related to transporter, signal transduction, and material and energy metabolism are involved in solvent tolerance. Optimization of the expression level of membrane proteins and engineering of membrane proteins are utilized to tackle the toxicity caused by organic solvents. Conclusions: Membrane proteins occupy a strikingly important position in microbial solvent tolerance. Further research on novel methods in membrane proteins, trade-offs among overexpression and toxicity of membrane proteins and solvent yield, and a direct relationship between signaling pathways and solvent tolerance will advance the utilization of organic solvent-tolerant microorganisms in biotechnology.

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Mechanistic Insight into Phenolic Compounds Toxicity and State-of-the-art Strategies for Enhancing the Tolerance of Escherichia coli to Phenolic Compounds

Cited by 3 publications

References 98 publications

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Antimicrobial and antibiofilm activities of chromone derivatives against uropathogenic Escherichia coli

An Overview of the Role of Membrane Proteins in Microbial Solvents Tolerance

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