Mechanisms underlying the nociception and paw oedema caused by injection of glutamate into the mouse paw

Beirith, Alessandra; Santos, Adair R.S.; Calixto, João B.

doi:10.1016/s0006-8993(01)03240-1

Cited by 299 publications

(289 citation statements)

References 46 publications

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“…The first phase is associated to direct stimulation of C-nociceptors, whereas the second phase reflects integration between peripheral (nociceptors) and central (spinal/ brainstem) signaling Capuano et al, 2009). Furthermore, it has been reported that the development of hyperalgesia due to injection of formalin involves glutamatergic system, such as N-methyl-D-aspartate (NMDA) receptors (Beirith et al, 2002;Luccarini et al, 2006). Pretreatment with LEO or LIN were able to block both phases of the formalin response.…”

Section: Discussionmentioning

confidence: 99%

“…NMDA can stimulate the production of a variety of intracellular second messengers, such as nitric oxide (NO) (Carlton et al, 1998). Beirith et al (2002) described that the nociceptive response induced by glutamate appears to involve peripheral, spinal and supraspinal sites of action and it is greatly mediated by both NMDA and non-NMDA receptors. In this regard, Batista et al (2008) demonstrated that antinociceptive effect of LIN .…”

Section: Discussionmentioning

confidence: 99%

“…The procedure used was similar to that previously described by Beirith et al (2002) with some alterations, including the local of application according to Quintans-Júnior et al (2010). A volume of 20 μL of glutamate (25 μM/paw prepared in phosphate buffered saline) was injected in the right upper lip (perinasal area), using a 27-gauge needle.…”

Section: Glutamate-induced Nociceptionmentioning

confidence: 99%

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Ocimum basilicum leaf essential oil and (-)-linalool reduce orofacial nociception in rodents: a behavioral and electrophysiological approach

Venâncio¹,

Marchioro²,

Estavam³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The present study investigated the antinociceptive effects of Ocimum basilicum L. (Lamiaceae) leaf essential oil (LEO) and (-)-linalool (LIN) in formalin (2%)-, glutamate (25 μM)-and capsaicin (2.5 µg)-induced orofacial nociception models in mice. The involvement of these substances was further evaluated on the neuronal excitability of the hippocampal dentate gyrus. Male mice (n=8/group) were pretreated separately with LEO and by LIN (50, 100, and 200 mg/kg, i.p.), morphine (5 mg/kg, i.p.) and vehicle (saline + Tween 80 0.2%), before injection of nociceptive agent into the right upper lip (perinasal area). The LEO and LIN reduced the nociceptive face-rubbing behaviour in both phases on formalin test. LEO and LIN, at high doses, produced significantly antinociceptive effect in the capsaicin and glutamate tests. In hippocampal slices, LEO inhibited the population spike generated by stimulation of the hylus (antidromic stimulation), with an IC50 of 0.1±0.05 mg/mL. This response was reversibly blocked by lidocaine (0.5 mg/mL), a known voltage-dependent sodium channel antagonist and by LIN (0.5 mg/mL). Our results suggest that LEO and LIN modulate neurogenic and inflammatory pain in the tests of orofacial nociception induced by formalin, capsaicin and glutamate. Part of these effects may be associated with decreased peripheral and central neuronal excitability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Glutamate-induced Nociceptionmentioning

confidence: 99%

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Ocimum basilicum leaf essential oil and (-)-linalool reduce orofacial nociception in rodents: a behavioral and electrophysiological approach

Venâncio¹,

Marchioro²,

Estavam³

et al. 2011

Rev. bras. farmacogn.

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“…Several reports have shown that NMDA receptors are present in both CNS and in the peripheral nerves (Carlton, 2001; Carlton & Coggeshall, 1999; Coggeshall & Carlton, 1998). In addition, peripheral administration of NMDA receptor antagonists can produce antinociceptive effects (Beirith et al., 2002; Davidson et al., 1997; Warncke et al., 1997). In this regard, RNA interference‐mediated gene silencing of peripheral NMDA receptors is a potential alternative for pain treatment because it provides sustained analgesic effects and avoids CNS side effects.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that certain N ‐methyl‐D‐aspartate (NMDA) receptor antagonists are useful analgesics for managing inflammatory (Beirith, Santos, & Calixto, 2002; Davidson, Coggeshall, & Carlton, 1997) and burning pain in humans (Warncke, Jorum, & Stubhaug, 1997). Nonetheless, the use of these antagonists is limited because of the numerous side effects resulting from antagonizing NMDA receptors in the central nervous system (CNS).…”

Section: Introductionmentioning

confidence: 99%

Lentiviral vector‐encoded microRNA‐based shRNA‐mediated gene knockdown of N‐methyl‐D‐aspartate receptors in skin reduces pain

Liu¹,

Cheng

Hung

et al. 2016

Brain and Behavior

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Background and Purpose RNA polymerase II promoters that drive the expression of rationally designed primary microRNA‐based shRNA, for example, shRNAmir, can produce more potent gene knockdown than RNA polymerase III promoters. Antagonists of peripheral N methyl‐D‐aspartate (NMDA) receptors that do not interfere with central glutamate processing would prevent the development of adverse central nervous system effects. Thus, in this study, we examined the effects of gene silencing and antinociception on formalin‐ and Complete Freund's adjuvant (CFA)‐induced pain in rats by subcutaneously injecting a lentiviral vector encoding a shRNAmir that targets the NR1 subunit of the NMDA receptor.MethodsRats received intradermal injections of different doses of NR1 shRNAmir at different time points before injection of formalin. Pain behavior was assessed by monitoring the paw flinch response, paw withdrawal threshold, and thermal withdrawal latency. We then analyzed NR1 messenger RNA and protein expression in skin and the L5 dorsal root ganglion (DRG).ResultsWe found that intradermal injection of 1, 5, and 10 μg of shRNAmir significantly inhibited flinch responses (p < .05). Administration of 5 μg of shRNAmir resulted in the attenuation of CFA‐induced mechanical allodynia, but did not affect the time spent on the rotarod. Real‐time polymerase chain reaction and western blotting revealed that NR1 mRNA and protein levels were significantly lower in all NR1 shRNAmir1 groups than in controls (p < .05). There was a significant reduction in the percentage of NR1‐ and pERK‐positive neurons in the DRG ipsilateral to shRNAmir treated paws (p < .05). The effect of antinociception and inhibition of NR1 expression by NR1 shRNAmir was evident on day 3 and persisted for 7 days after injection of 5 μg of vector.ConclusionPeripheral administration of the vector‐encoded NR1 shRNAmir is a promising therapy for persistent inflammatory pain.

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Glutamate Receptors

Cairns

2009

Peripheral Receptor Targets for Analgesia

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Mechanisms underlying the nociception and paw oedema caused by injection of glutamate into the mouse paw

Cited by 299 publications

References 46 publications

Ocimum basilicum leaf essential oil and (-)-linalool reduce orofacial nociception in rodents: a behavioral and electrophysiological approach

Ocimum basilicum leaf essential oil and (-)-linalool reduce orofacial nociception in rodents: a behavioral and electrophysiological approach

Lentiviral vector‐encoded microRNA‐based shRNA‐mediated gene knockdown of N‐methyl‐D‐aspartate receptors in skin reduces pain

Glutamate Receptors

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