Mechanisms of vancomycin resistance in Staphylococcus aureus

Gardete, Susana; Tomasz, Alexander

doi:10.1172/jci68834

Cited by 436 publications

(340 citation statements)

References 66 publications

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“…aureus developed resistance shortly after the introduction of penicillin in 1940s, followed by methicillin resistance in 1961. Hitherto, numbers of publication have been reported concerning the resistance of S. aureus isolates to diverse spectrum of antibiotics [3,4] . S. aureus is causing a concern due to its ability to become resistant to antibiotics via acquired by horizontal transfer of genes and chromosomal mutation [5] .…”

Section: Introductionmentioning

confidence: 99%

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hızlısoy¹,

Onmaz²,

Karadal³

et al. 2018

Kafkas Univ Vet Fak Derg

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In this study, the investigation of the antibiotic resistance gene profiles of Staphylococcus aureus isolates from foods of animal origin was aimed. Totally, 95 S. aureus strains, obtained during a period between 2009 and 2012, from culture collection of the Food Hygiene and Technology Laboratory, were examined. The isolates were confirmed by phenotypic tests and PCR. The antibiotic susceptibilities of the isolates were analyzed by disc diffusion method and the minimal inhibition concentrations of the antibiotics were determined by E test. PCR were also utilized for determining the presence of resistance genes including blaZ, ermA, ermC, tetK, tetM, mecA, VanA, VanB, VatA, VatB and aacA-aphD. Resistance to penicillin, tetracycline, vancomycin, erythromycin, cefoxitin, gentamycin and quinupristin-dalfopristin were evident as 81.1%, 28.4%, 18.9%, 17.9%, 9.4%, 9.4% and 3.2% respectively. E test results were compatible with the disc diffusion method. Multidrug resistance was observed from 29.5% of S. aureus isolates. Positive compatibility was observed between conventional methods and PCR for the resistance of the isolates, except for vancomycin. In addition, all of the tested isolates found to include a resistance gene for at least one antibiotic. In conclusion, more efficient interventions must be followed to control the redundant use of antibiotics in veterinary practice. Furthermore, appropriate control measures are needed to be implemented to reduce contamination and the spread of multiresistant S. aureus strains. Keywords: Antibiotic resistance, Animal origin foods, Multidrug resistance, Resistance genes, Staphylococcus aureus Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus'ların Antibiyotik Dirençlilik Gen Profilleri

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Section: Introductionmentioning

confidence: 99%

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hızlısoy¹,

Onmaz²,

Karadal³

et al. 2018

Kafkas Univ Vet Fak Derg

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“…As a result, the rate of vancomycin diffusion decreases [45]. Another mechanism was identical to that seen in vancomycin-resistant Enterococcus faecium that harbors the vanA operon which contains 5 genes [46]. To determine the mechanism of acquirement of reduced vancomycin susceptibility of VISA strains, we detected the Van gene which was implicated in generation of vancomycin resistance [47].…”

Section: Discussionmentioning

confidence: 99%

Asymptomatic colonization of Staphylococcus aureus with intermediate resistance to vancomycin harboring vanB resistance gene

Daghistani

Shquirat

Al-kharabsha

et al. 2017

Asian J Pharm Clin Res

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Objective: Vancomycin has been used worldwide due to empirical therapy against methicillin-resistant Staphylococcus aureus infections. As a result a selective pressure that favors the outgrowth of vancomycin intermediate S. aureus clones will be created. This study was carried out to evaluate vancomycin resistance pattern of S. aureus in Jordan.Methods: A total of 1179 samples, including 566 (48%) from human and 613 (52%) from animals were examined for the presence of S. aureus using standard biochemical tests and polymerase chain reaction (PCR) amplification of coa gene. Resistance to antibiotics was determined by the disk diffusion method. Methicillin resistance strains were tested for vancomycin resistance by minimal inhibitory concentration (MIC), E-test, and the results were confirmed by amplification of van genes, and Pulsed-field gel electrophoresis (PFGE). Results:The prevalence of S. aureus among human source was: 19.35%, 14%, and 8.8% for nasal, nail, and skin, respectively, and for animal sources 27.3%, 5.51%, and 15.86% for milk, nasal, and meat, respectively. Four VISA strains (1.87%) were found to colonize human nares, nails, and skin with vancomycin MIC of 4-8 μg/ml. Van B resistance gene was detected and PFGE with SmaI-digested VISA genomic DNA revealed two different pulsotypes. Conclusion:This is believed to be the first report of VISA strains containing vanB gene isolated from a routine carriage survey. Effective screening directed to persons colonized with VISA should therefore be a priority.

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“…Yapılan çalışmalarda Öksüz ve arkadaşları 14 Kullanıma giren yeni antibiyotiklere rağmen MRSA enfeksiyonla¬rında temel tedavi seçeneği glikopeptidlerdir. Glikopeptid direnci ilk kez 2002 yılında ortaya çıkmış olup, son yıllarda dünyanın değişik bölgelerinde giderek artan oranlarda VISA/hVISA izolatları rapor edilmektedir 16,17 . VISA izolatlarının rutin antimikrobiyal duyarlılık testleri ile belirlenememesi ve vankomisinin oldukça yaygın olarak kullanılıyor olması, ülkemizde belirli aralıklarla azalmış vankomisin duyarlılığı araştırılmasını gerekli kılmaktadır.…”

Section: Discussionunclassified

Metisiline Dirençli Staphylococcus aureus İzolatlarının Antibiyotik Direnci ve Azalmış Vankomisin Duyarlılığının Araştırılması: Çok Merkezli Bir Çalışma

Çıkman¹,

Aydın

Gülhan³

et al. 2015

Mikrobiyol Bul

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Mechanisms of vancomycin resistance in Staphylococcus aureus

Cited by 436 publications

References 66 publications

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Asymptomatic colonization of Staphylococcus aureus with intermediate resistance to vancomycin harboring vanB resistance gene

Metisiline Dirençli Staphylococcus aureus İzolatlarının Antibiyotik Direnci ve Azalmış Vankomisin Duyarlılığının Araştırılması: Çok Merkezli Bir Çalışma

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