Mechanisms of toxicities of some detergents added to a diet and of the ameliorating effect of dietary fiber in the rat.

Mechanisms of adverse effect of air-oxidized soy bean oil-feeding in rats.

“…On the other hand, the release of sucrase in NaTC-RBS perfusion medium containing unoxidized soy bean oil at the 1% level kept a constant, which was similar to those levels in the second 30-min RBS perfusion and in the NaTC-RBS perfusion. The increased release of sucrase due to the oxidized soy bean oil perfusion corresponded to that due to the Tween 20 or 60 perfusion observed in the previous study (8), indicating the oxidized soy bean oil solubilized or exfoliated the brush-border membrane of the small intestine. The suggestion that the mechanism of production of the adverse effects caused by oxidized soy bean oil feeding was similar to that on Tween 20 or 60 feeding (5)(6)(7)(8), was supported by the experimental results obtained from the present study.…”

“…The increased release of sucrase due to the oxidized soy bean oil perfusion corresponded to that due to the Tween 20 or 60 perfusion observed in the previous study (8), indicating the oxidized soy bean oil solubilized or exfoliated the brush-border membrane of the small intestine. The suggestion that the mechanism of production of the adverse effects caused by oxidized soy bean oil feeding was similar to that on Tween 20 or 60 feeding (5)(6)(7)(8), was supported by the experimental results obtained from the present study. The latter demonstrated that the mechanism of generation of the adverse effects arising from oxidized soy bean oil feeding was geared to a disturbance in gastrointestinal functions, suggesting the possibility that the primary cause was solubilizing or exfoliating effects of oxidized soy bean oil on the brush-border membrane of the small intestine.…”

Mechanisms of adverse effect of amaranth feeding in the rat.

“…The release of sucrase in the control group lasted at a constant rate for 120min after the first 30min RBS perfusion. The constant liberation rate of sucrase activity was corresponding to the physiological cell desquamation (6,17). The releasing patterns of maltase and alkaline phosphatase as well as protein in the control group were exactly similar to that of sucrase (6).…”

“…The constant liberation rate of sucrase activity was corresponding to the physiological cell desquamation (6,17). The releasing patterns of maltase and alkaline phosphatase as well as protein in the control group were exactly similar to that of sucrase (6). The sucrase activity in the second 30min RBS-perfusate was adopted as a standard for the relative activities of sucrase released by RBS and amaranth-RBS perfusion for the last 90min.…”

“…In the previous studies (3)(4)(5)(6) we reported that the mechanisms were the exfoliating or solubilizing effects of these detergents in the brush border membrane of the small intestine, and that the dietary fiber prevented these effects. The toxicities of amaranth feeding as well as those of non-ionic surface-active agent feeding were prevented by the concurrent feeding of dietary fiber (7,8).…”