Mechanisms of the Separation and Transport of Polymer Systems in Chromatographic Media

Tijssen, R.; Bos, Jaap.

doi:10.1007/978-94-011-2686-1_15

Cited by 9 publications

(5 citation statements)

References 110 publications

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“…Those forces change the uniformity of the concentration profile of the particles over the channel cross section, thus favoring certain velocities and influencing the retention. With all the effects included, the retention curve especially for colloids is more complicated than eq 3, as was described by Tijssen . This is also apparent from experimental results of Small, and Noel interpreted by Ploehn, the latter presenting trendlines with several inflex points.…”

Section: Theorymentioning

confidence: 71%

“…They present a corrected approximation for a cylindrical tube but no explicit result for a planar geometry. Furthermore, the above formulas do not reflect radial forces such as the hydrodynamic “tubular pinch” effect, , which occurs at higher velocities, and electrostatic and electrokinetic lift forces acting upon charged particles in mainly aqueous solutions . Those forces change the uniformity of the concentration profile of the particles over the channel cross section, thus favoring certain velocities and influencing the retention.…”

Section: Theorymentioning

confidence: 99%

“…Observation of possible peak distortion in such channels would assist the future integration of microdetectors. Further reducing the channel depth should also allow a new separation mechanism for polymers by chain reptation as proposed by Tijssen in 1992.…”

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confidence: 99%

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A Chip System for Size Separation of Macromolecules and Particles by Hydrodynamic Chromatography

Chmela

Tijssen²,

Blom³

et al. 2002

Anal. Chem.

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128

107

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For the first time, a miniaturized hydrodynamic chromatography chip system has been developed and tested on separation of fluorescent nanospheres and macromolecules. The device can be applied to size characterization of synthetic polymers, biopolymers, and particles, as an attractive alternative to the classical separation methods such as size exclusion chromatography or field-flow fractionation. The main advantages are fast analysis, high separation efficiency, negligible solvent consumption, and easy temperature control. The prototype chip contains a rectangular flat separation channel with dimensions of 1 microm deep and 1000 microm wide, integrated with a 300-pL injector on a silicon substrate. The silicon microtechnology provides precisely defined geometry, high rigidity, and compatibility with organic solvents or high temperature. All flows are pressure driven, and a specific injection system is employed to avoid excessive sample loading times, demonstrating an alternative way of lab-on-a-chip design. Separations obtained in 3 min show the high performance of the device and are also the first demonstration of flat channel hydrodynamic chromatography in practice.

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Section: Theorymentioning

confidence: 71%

Section: Theorymentioning

confidence: 99%

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A Chip System for Size Separation of Macromolecules and Particles by Hydrodynamic Chromatography

Chmela

Tijssen²,

Blom³

et al. 2002

Anal. Chem.

Self Cite

128

107

View full text Add to dashboard Cite

show abstract

“…(5a), m is a parameter that depends on the pore shape. Hence, for spherical molecules, m is equal to 1, 2 or 3, for slit shaped pores, long cylindrical pores, and spherical or conical pores, respectively [8,14]. In SEC, a partition constant, KSEC, i, can be measured from the retention times of the three species i, perm and excl:…”

Section: Kseexcl Eq --Cp'excle---~q --0 (3) Coexcl Eqmentioning

confidence: 99%

Experimental validation of the stochastic theory of size-exclusion chromatography: Retention on single and coupled columns

et al. 2003

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The experimental validation of the recently developed stochastic model of size-exclusion chromatography (SEC), interpreted as a random process in which macromolecules undergo a continuous exchange process between the moving zone between the packing particles and the stagnant zone inside the particles, is attempted on the basis of an extended data set of experimental chromatograms of polystyrene standards, with molar masses ranging from 0.16 to 2200 kDa, on five different columns with widely different pore sizes. Several features of experimental chromatograms of standards eluting within or outside the permeation range, such as band broadening, peak tailing and peak splitting are qualitatively interpreted at the light of the stochastic theory of SEC. Furthermore, some aspects such as the influence of sample polydispersity on peak broadeningand of the exclusion effect in the movingzone (hydrodynamic chromatographic effect) on retention are discussed.\ud A new chemometric procedure was developed for estimatingthe KSEC equilibrium distribution constant without a priori assumptions regarding the extraparticle column volume, V0 , and the total pore volume Vp . This procedure allowed to obtain an unified description of the large retention data set over different columns. All retention data belonging to calibration ranges of the various columns, followed quite similar calibration plots, under the form of KSEC = (1 – r)m, where r is the ratio of macromolecule size to pore size, with m values close to 3 for all columns. A similar approach was able to represent the behaviour of two connected column in series, with consistent values of the pertinent parameters, which validates the stochastic theory of SEC with two pore types of different sizes. Finally, this two-pore model and a mixed SEC-hydrodynamic chromatography model were compared to interpret retention data on single columns around the exclusion limits. The practical application of these approaches in routine analysis, e.g. for monitoring the column degradation during its use, is mentioned

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“…As the number of particle−wall interactions becomes a dominant factor with decreasing channel depth and increasing particle diameter, we are intrigued to find out how this will influence the observed mean particle velocity and the extent of band broadening. Based on hydrodynamic mechanisms for the laminar flow of colloidal particles, − it can be assumed that the linear velocity field will cause a rotation of the particles, thereby inducing an upward or downward lift toward one of the two surfaces. As these have a different velocity in the case of a shear-driven flow channel, a reduced or increased average velocity may be expected.…”

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confidence: 99%

High-Velocity Transport of Nanoparticles through 1-D Nanochannels at Very Large Particle to Channel Diameter Ratios

et al. 2004

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We explore the possibility of generating high-velocity flows of nanoparticles through flat-rectangular nanochannels, which are only 50% deeper than the diameter of the particles. Using the shear-driven flow principle, 200-nm particles can, for example, be transported through a 300-nm-deep channel at velocities up to 35 mm/s (upper limit of our current setup). Working under high-pH conditions, the velocity of the carboxylated nanoparticles still respects the small-molecule velocity law, despite the high degree of confinement to which the particles are subjected. The high degree of confinement is also found to lead to a reduced band broadening. When injecting sharply delimited particle plugs, the plate heights observed for the flow of 0.2-microm particles through a 0.3-microm channel (with plate heights of the order of 1-2 microm) are, for example, approximately 1 order of magnitude smaller than for the flow of 1.0-microm particles through a 1.4-microm channel. It is also found that the band broadening is, within its statistical variation, independent of the fluid velocity over a large range of particle velocities (5-35 mm/s). The flow method distinguishes itself from pressure-driven field-flow fractionation and hydrodynamic chromatography in that the mean particle velocity is independent of the particle size over the entire range of possible particle to channel diameter ratios.

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Mechanisms of the Separation and Transport of Polymer Systems in Chromatographic Media

Cited by 9 publications

References 110 publications

A Chip System for Size Separation of Macromolecules and Particles by Hydrodynamic Chromatography

A Chip System for Size Separation of Macromolecules and Particles by Hydrodynamic Chromatography

Experimental validation of the stochastic theory of size-exclusion chromatography: Retention on single and coupled columns

High-Velocity Transport of Nanoparticles through 1-D Nanochannels at Very Large Particle to Channel Diameter Ratios

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