Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters

Fararh, Khalid; Atoji, Yasuro; Shimizu, Yasutake; Shiina, Takahiko; Nikami, Hideki; Takewaki, Tadashi

doi:10.1016/j.rvsc.2004.03.002

Cited by 130 publications

(99 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NSO (400 mg/kg by gastric gavage) given daily reduced blood glucose from 391 ± 3.0 mg/dl before treatment to 325 ± 4.7, 246 ± 5.9, 208 ± 2.5 and 179 ± 3.1 mg/dl after the first, second, third and fourth week of treatment, respectively. Hepatic glucose production from gluconeogenic precursors (alanine, glycerol and lactate) was significantly lower in treated hamsters indicating decreased hepatic gluconeogenesis [49]. Similar effect was observed when TQ (50 mg/kg daily) was given to STZ-induced diabetic hamsters, 4 weeks after induction of diabetes.…”

Section: Antidiabetic Activity In Stz Induced Diabetic Hamsterssupporting

confidence: 63%

“…Anti-diabetic action of N. sativa is at least partly mediated through decreased liver gluconeogenesis [60]. Fararh showed that hepatic glucose production from gluconeogenic precursors (alanine, glycerol and lactate) was significantly lower in treated hamsters indicating it decreases hepatic gluconeogenesis [49]. Le et al demonstrated that in vivo treatment with petroleum ether extract of N. sativa, resulted in greater dose-dependent activation of MAPK p44/42erk and PKB in response to insulin in hepatocytes, which suggests that in vivo treatment with N. sativa extract exerts an insulin-sensitizing action by enhancing the activity of the two major intracellular signal transduction pathways of the hormone's receptor [38].…”

Section: Possible Mechanisms Of Antidiabetic Activitymentioning

confidence: 99%

See 1 more Smart Citation

Antidiabetic Properties of a Spice Plant Nigella sativa

Mathur

2011

J Endocrinol Metab

View full text Add to dashboard Cite

Seeds of Nigella sativa (black cumin/kalonji) used in pickles as spice, have also been traditionally used in treatment of many diseases including diabetes and hypertension. Among many activities exhibited by N. sativa and its constituents in animal experiments, antidiabetic property is most important. Thymoquinone (TQ), a volatile oil, is one of its active constituents but antidiabetic activity has also been shown by its aqueous extract and defatted extract. N. sativa may be beneficial in diabetic individuals and those with glucose intolerance as it reduces appetite, glucose absorption in intestine, hepatic gluconeogenesis, blood glucose level, cholesterol, triglycerides, body weight and simulates glucose induced secretion of insulin from beta-cells in pancreas; improves glucose tolerance as efficiently as metformin; yet it has not shown significant adverse effects and has very low toxicity. In streptozotocin (STZ) induced diabetic rats it causes gradual partial regeneration of pancreatic beta-cells, increases the lowered serum insulin concentrations and decreases the elevated serum glucose. N. sativa has antioxidant activity and protective role of TQ against development of type I diabetes may be via NO inhibitory pathway. It also exerts an insulin-sensitizing action in hepatocytes. Seeds of N. sativa have been safely consumed by human patients in many clinical trials which however were not aimed to assess its antidiabetic activity. In future clinical studies may show potential of N. sativa, its constituents or their synthetic analogues, in prevention and control of diabetes.

show abstract

Section: Antidiabetic Activity In Stz Induced Diabetic Hamsterssupporting

confidence: 63%

Section: Possible Mechanisms Of Antidiabetic Activitymentioning

confidence: 99%

Antidiabetic Properties of a Spice Plant Nigella sativa

Mathur

2011

J Endocrinol Metab

View full text Add to dashboard Cite

show abstract

“…The in vivo antihyperglycemic activity of this extract is well-known (Al-Awadi et al, 1985, 1987Al Hader et al, 1993;Labhal et al, 1999;Meral et al, 2001;El-Dakhakhny., 2002;Farah et al, 2002;Kanter et al, 2003). Although this action has been mainly attributed to insulinotropic effects, observed in animal studies as well as in isolated pancreatic islets (Rchid et al, 2004), the contribution of peripheral sites of action has been suggested (Al-Awadi et al, 1991;Fararh et al, 2004). The purpose of this study was to use cell-based assays of antidiabetic activity to elucidate the cellular site(s) of action of NS biological activity.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic Activity ofNigella sativa. Seed Extract in Cultured Pancreatic β-cells, Skeletal Muscle Cells, and Adipocytes

Benhaddou-Andaloussi

Martineau

Spoor

et al. 2008

Pharmaceutical Biology

View full text Add to dashboard Cite

The seeds of Nigella sativa L. (NS), a plant of the Runanculaceae family, are used in traditional medicine in North Africa and the Middle East for the treatment of diabetes. Despite widespread use and a number of scientific studies, the target tissues and cellular mechanisms of action of this plant product are not well understood. This study evaluated the effects of NS seed crude ethanol extract on insulin secretion in INS832/13 and βTC-tet lines of pancreatic β-cells and on glucose disposal by C2C12 skeletal muscle cells and 3T3-L1 adipocytes. An 18-h treatment with NS amplified glucose-stimulated insulin secretion by more than 35% without affecting sensitivity to glucose. NS treatment also accelerated β-cell proliferation. An 18-h treatment with NS increased basal glucose uptake by 55% (equivalent to approximately two-fold the effect of 100 nM insulin) in muscle cells and approximately by 400% (equal to the effect of 100 nM insulin) in adipocytes; this effect was perfectly additive to that of insulin in adipocytes. Finally, NS treatment of pre-adipocytes undergoing differentiation accelerated triglyceride accumulation comparably with treatment with 10 µM rosiglitazone. It is concluded that the well-documented in vivo antihyperglycemic effects of NS seed extract are attributable to a combination of therapeutically relevant insulinotropic and insulin-like properties.

show abstract

“…However, it has been proven that N. sativa oil can inhibit DNA synthesis by inhibiting histone deacetylase (HDAC) enzyme interacting with the chromosomes (Suthar et al, 2010). In addition, it has an immunopotentiating effect through stimulating phagocytic activity and phagocytic index of peritoneal macrophages or activating lymphocytes in streptozotocin (STZ)-induced diabetic hamsters (Fararh et al, 2004). Various studies have shown that administration of N. sativa seed extract and its components as oral or intraperitoneally represents a low level of cytotoxicity in rats and mice (Badary, 1998;El Daly, 1998;Khanna et al, 1993;Salem & Hossain, 2000).…”

Section: Discussionmentioning

confidence: 99%

Leishmanicidal and cytotoxic activities ofNigella sativaand its active principle, thymoquinone

Mahmoudvand

Tavakoli

Sharififar

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Leishmaniasis is a complex disease with a broad spectrum of clinical presentations. Objective: We evaluated the anti-leishmanial effects of Nigella sativa L. (Ranunculaceae) against Leishmania tropica and Leishmania infantum with an in vitro model. Materials and methods: Antileishmanial effects of essential oil and methanolic extract of N. sativa (0-200 mg/mL) and thymoquinone (0-25 mg/mL) on promastigotes of both species and their cytotoxicity activities against murine macrophages were evaluated using the MTT assay at 24, 48, and 72 h. Moreover, their leishmanicidal effects against amastigotes were investigated in a macrophage model, for 48 and 72 h. Results: The findings showed that essential oil (L. tropica IC 50 9.3 mg/mL and L. infantum IC 50 11.7 mg/mL) and methanolic extract (L. tropica IC 50 14.8 mg/mL and L. infantum IC 50 15.7 mg/mL) of N. sativa, particularly thymoquinone (L. tropica IC 50 1.16 mg/mL and L. infantum IC 50 1.47 mg/ mL), had potent antileishmanial activity on promastigotes of both species after 72 h. In addition, essential oil (L. tropica IC 50 21.4 mg/mL and L. infantum IC 50 26.3 mg/mL), methanolic extract (L. tropica IC 50 30.8 mg/mL and L. infantum IC 50 34.6 mg/mL), and thymoquinone (L. tropica IC 50 2.1 mg/mL and L. infantum IC 50 2.6 mg/mL) mediated a significant decrease in the growth rate of amastigote forms of both species. Thymoquinone (CC 50 38.8 mg/mL) exhibited higher cytotoxic effects against murine macrophages than the other extracts. Conclusion: N. sativa, especially its active principle, thymoquinone, showed a potent leishmanicidal activity against L. tropica and L.infantum with an in vitro model.

show abstract

Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters

Cited by 130 publications

References 20 publications

Antidiabetic Properties of a Spice Plant Nigella sativa

Antidiabetic Properties of a Spice Plant Nigella sativa

Antidiabetic Activity ofNigella sativa. Seed Extract in Cultured Pancreatic β-cells, Skeletal Muscle Cells, and Adipocytes

Leishmanicidal and cytotoxic activities ofNigella sativaand its active principle, thymoquinone

Contact Info

Product

Resources

About