Mechanisms of Resistance to Chloramphenicol in Pseudomonas putida KT2440

Fernández, Matilde; Conde, Susana; Torre, J. de la; Molina-Santiago, Carlos; Ramos, Juan L.; Duque, Estrella

doi:10.1128/aac.05398-11

Cited by 99 publications

(75 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bashir et al [22] and Akinduti et al [23] also reported in their study carried out in Kashmir and Abeokuta respectively that P. aeruginosa is a multidrug resistant organism that is notoriously resistant to several antibiotic classes. And the multidrug resistant nature of P. aeruginosa isolates has been confirmed by Fernandez et al [16] in the Americas as well. The prevalence of AmpC β-lactamase positive P. aeruginosa isolates was 25 %.…”

Section: Discussionsupporting

confidence: 49%

“…Ceftazidime, ofloxacin, ciprofloxacin and gentamicin showed appreciable level of antimicrobial activity against the P. aeruginosa isolates at the rate of 83.3 %, 83.3 %, 91.7 %, and 91.7 % respectively. Reports around the world show that P. aeruginosa is notorious for its resistance to antibiotics [16][17][18]. Our result on the antimicrobial susceptibility patterns of P. aeruginosa isolates to antibiotics is similar to the work of Franco et al [19] The prevalence of AmpC-positive P. aeruginosa isolates in this study is lower than the report of Abd El-Baky et al [24] who reported higher prevalence of AmpC-positive P. aeruginosa (72.4 %) in their study.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Chika¹

2017

JMEN

View full text Add to dashboard Cite

The possible contamination of food and/or food-producing animals with multidrug resistant bacteria including AmpC-producing Pseudomonas aeruginosa is considered a potential source for the wide dissemination of AmpC β-lactamase in the community. This portends public health risks for the populace -owing to the multidrug resistant nature of these organisms. There is paucity of data on the prevalence of AmpCproducing bacteria in Abakaliki, Nigeria -which was why this study was carried out. A total of 40 feacal swab samples from cow dung in a local abattoir were bacteriologically examined for the isolation and antimicrobial susceptibility testing of P. aeruginosa isolates using standard microbiological procedures on cetrimide selective agar and Kirby-Bauer disk diffusion method respectively. AmpC β-lactamase was phenotypically confirmed using the ceftazidime-imipenem antagonism test (CIAT). A total of 12 P. aeruginosa isolates were recovered from the samples; and they showed varied levels of resistance to the tested antibiotics especially to cefoxitin, ertapenem, oxacillin, amikacin and cefotaxime. Among the 12 P. aeruginosa isolates, AmpC β-lactamase was phenotypically detected in 3 (25%) isolates by the CIAT method. This study has presumptively shown that AmpC β-lactamase-producing P. aeruginosa isolates occur in abattoir. Thus, molecular characterization of the genes that encode AmpC β-lactamase production in this organism is crucial for a reliable epidemiological investigation into the possible emergence and dissemination of AmpC positive bacteria in the community. monitoring and possibly a ban on the use of antibiotics in the production and/or rearing of food producing animals in order to assuage the emergence and spread of drug resistant bacteria in the community [7,19,21]. Thus, this study presumptively detected the occurrence of AmpC-producing P. aeruginosa isolates from feacal matter of cow in a local abattoir. Keywords: Materials and Methods Sample collection and processingA total of forty (40) swab samples were collected from feacal matter of cow in a local abattoir using sterile swab sticks. After collection, each of the swab sticks was returned into their respective containers, labeled and transported to the Microbiology Laboratory Unit of Ebonyi State University, Abakaliki for bacteriological analysis. Isolation and identification of Pseudomonas aeruginosaCetrimide selective agar (Oxoid, UK) containing 10 ml of glycerol was used for the selective isolation of P. aeruginosa from the test samples. Each of the feacal swab samples was inoculated in 5 ml double strength of freshly prepared nutrient broth (Oxoid, UK) and incubated for 18-24 hours at 30 o C. A loopful of the broth culture was aseptically streaked on cetrimide selective agar plates; and the plates were incubated at 30 o C 18-24 hours. Suspected colonies were inoculated onto freshly prepared cetrimide selective agar for the isolation of pure cultures of P. aeruginosa. P. aeruginosa produces greenish/bluish pigmentation on cetrimide selec...

show abstract

Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Chika¹

2017

JMEN

View full text Add to dashboard Cite

show abstract

“…This approach inserts the gene in a fixed position on the genome, after the gene glmS , which has previously been shown to be innocuous to the cell physiology (Lambertsen, Sternberg, & Molin, 2004). Using this method we avoided the natural chloramphenicol resistance of P. putida KT2440 as a selection marker, which would introduce a bias in the transposon insertion sequencing results for mutants involved in the chloramphenicol resistance, such as the efflux pump TtgABC (Fernandez, Conde et al, 2012). The use of alternative carbon sources, like citrate, that only the receptor strain could degrade was also avoided, and the developed method thereby enabled the use of LB in the creation of the library.…”

Section: Resultsmentioning

confidence: 99%

“…Some of the changes that can occur in the membrane, when bacteria are exposed to hydrophobic solvents, include vesicle formation, alteration of phospholipid composition, and reduced permeability of the cell membrane (Heipieper & De Bont, 1994; Nicolaou, Gaida, & Papoutsakis, 2010; Ramos et al, 2002). The mechanisms behind tolerance in the model strain P. putida KT2440 has also been studied for different compounds (Benndorf, Thiersch, Loffhagen, Kunath, & Harms, 2006; Domínguez‐Cuevas, González‐Pastor, Marqués, Ramos, & de Lorenzo, 2006; Fernandez, Conde et al, 2012; Fernandez, Niqui‐Arroyo, Conde, Ramos, & Duque, 2012; Roca, Rodríguez‐Herva, Duque, & Ramos, 2008; Santos, Benndorf, & Sá‐Correia, 2004). …”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Calero

Jensen

Bojanovič

et al. 2017

Biotech & Bioengineering

View full text Add to dashboard Cite

The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found toward p‐coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome‐wide approach, Tn‐seq. Libraries containing a large number of miniTn5‐Km transposon insertion mutants were grown in the presence and absence of p‐coumaric acid, and the enrichment or depletion of mutants was quantified by high‐throughput sequencing. Several genes, including the ABC transporter Ttg2ABC and the cytochrome c maturation system (ccm), were identified to play an important role in the tolerance toward p‐coumaric acid of this bacterium. Most of the identified genes were involved in membrane stability, suggesting that tolerance toward p‐coumaric acid is related to transport and membrane integrity.

show abstract

“…pKA2 was then transferred from E. coli S17-1 to P. putida KT2440 by biparental mating (46), and the transconjugants were selected on LB plates containing kanamycin (100 mg liter Ϫ1 ) and chloramphenicol (50 mg liter Ϫ1 ). Kanamycin resistance of the transconjugants originated from pK18mobsacB, whereas chloramphenicol resistance was (47). This method selected the first-crossover mutants of KT2440, which had the pKA2 sequence inserted into the genome by homologous recombination; pKA2 cannot replicate in KT2440.…”

Section: Methodsmentioning

confidence: 99%

Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via γ-Glutamylanilide

et al. 2013

View full text Add to dashboard Cite

Acinetobacter sp. strain YAA has five genes (atdA1 to atdA5) involved in aniline oxidation as a part of the aniline degradation gene cluster. From sequence analysis, the five genes were expected to encode a glutamine synthetase (GS)-like protein (AtdA1), a glutamine amidotransferase-like protein (AtdA2), and an aromatic compound dioxygenase (AtdA3, AtdA4, and AtdA5) (M. Takeo, T. Fujii, and Y. Maeda, J. Ferment. Bioeng. 85:17-24, 1998). A recombinant Pseudomonas strain harboring these five genes quantitatively converted aniline into catechol, demonstrating that catechol is the major oxidation product from aniline. To elucidate the function of the GS-like protein AtdA1 in aniline oxidation, we purified it from recombinant Escherichia coli harboring atdA1. The purified AtdA1 protein produced gamma-glutamylanilide (␥-GA) quantitatively from aniline and L-glutamate in the presence of ATP and MgCl 2 . This reaction was identical to glutamine synthesis by GS, except for the use of aniline instead of ammonia as the substrate. Recombinant Pseudomonas strains harboring the dioxygenase genes (atdA3 to atdA5) were unable to degrade aniline but converted ␥-GA into catechol, indicating that ␥-GA is an intermediate to catechol and a direct substrate for the dioxygenase. Unexpectedly, a recombinant Pseudomonas strain harboring only atdA2 hydrolyzed ␥-GA into aniline, reversing the ␥-GA formation by AtdA1. Deletion of atdA2 from atdA1 to atdA5 caused ␥-GA accumulation from aniline in recombinant Pseudomonas cells and inhibited the growth of a recombinant Acinetobacter strain on aniline, suggesting that AtdA2 prevents ␥-GA accumulation that is harmful to the host cell.

show abstract

Mechanisms of Resistance to Chloramphenicol in Pseudomonas putida KT2440

Cited by 99 publications

References 65 publications

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via γ-Glutamylanilide

Contact Info

Product

Resources

About