Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via γ-Glutamylanilide

Takeo, Masahiro; Ohara, Akira; Sakae, Shinji; Okamoto, Yasuhiro; Kitamura, Chitoshi; Kato, Dai‐ichiro; Negoro, Seiji

doi:10.1128/jb.00397-13

Cited by 30 publications

(40 citation statements)

References 57 publications

(81 reference statements)

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“…To test its function, the plasmid pBBRQR harbouring adoQTA1A2BR was introduced into Pseudomonas putida KT2440-DcatA (in which both catechol dioxygenase genes were deleted) (Takeo et al, 2013). Gas chromatographymass spectrometry (GC-MS) analysis showed that 4-IA was transformed into 4-IPC (Supporting Information Fig.…”

Section: Genome Features Of Three Ipu-mineralizing Sphingomonads and mentioning

confidence: 99%

Comparative genomic analysis of isoproturon‐mineralizing sphingomonads reveals the isoproturon catabolic mechanism

Yan

et al. 2016

Environmental Microbiology

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SummaryThe worldwide use of the phenylurea herbicide, isoproturon (IPU), has resulted in considerable concern about its environmental fate. Although many microbial metabolites of IPU are known and IPU-mineralizing bacteria have been isolated, the molecular mechanism of IPU catabolism has not been elucidated yet. In this study, complete genes that encode the conserved IPU catabolic pathway were revealed, based on comparative analysis of the genomes of three IPUmineralizing sphingomonads and subsequent experimental validation. The complete genes included a novel hydrolase gene ddhA, which is responsible for the cleavage of the urea side chain of the IPU demethylated products; a distinct aniline dioxygenase gene cluster adoQTA1A2BR, which has a broad substrate range; and an inducible catechol meta-cleavage pathway gene cluster adoXEGKLIJC. Furthermore, the initial mono-N-demethylation genes pdmAB were further confirmed to be involved in the successive Ndemethylation of the IPU mono-N-demethylated product. These IPU-catabolic genes were organized into four transcription units and distributed on three plasmids. They were flanked by multiple mobile genetic elements and highly conserved among IPUmineralizing sphingomonads. The elucidation of the molecular mechanism of IPU catabolism will enhance our understanding of the microbial mineralization of IPU and provide insights into the evolutionary scenario of the conserved IPU-catabolic pathway.

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Section: Genome Features Of Three Ipu-mineralizing Sphingomonads and mentioning

confidence: 99%

Comparative genomic analysis of isoproturon‐mineralizing sphingomonads reveals the isoproturon catabolic mechanism

Yan

et al. 2016

Environmental Microbiology

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“…This enzyme is capable of catalyzing the production of γ-glutamylaniline in an ATP-dependent manner (Fig. 6a) [80]. AtdA1 was also found to act on aniline derivatives such as 2-, 3-, and 4-chloroaniline and 2-, 3-, and 4-methylaniline.…”

Section: Introductionmentioning

confidence: 99%

The many roles of glutamate in metabolism

Walker

Donk

2016

Journal of Industrial Microbiology and Biotechnology

141

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The amino acid glutamate is a major metabolic hub in many organisms and as such is involved in diverse processes in addition to its role in protein synthesis. Nitrogen assimilation, nucleoside, amino acid, and cofactor biosynthesis, as well as secondary natural product formation all utilize glutamate in some manner. Glutamate also plays a role in the catabolism of certain amines. Understanding glutamate's role in these various processes can aid in genome mining for novel metabolic pathways or the engineering of pathways for bioremediation or chemical production of valuable compounds.

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“…Through sequence analysis, one gene cluster containing 14 catabolic genes for complete aniline degradation was identified. The first six genes (dcaQTA1A2BR) encode a Lglutamate-based oxidative deamination pathway which transforms aniline to catechol (Takeo et al 2013). The rest of the eight genes in the cluster encode the lower pathway featured by a 2,3-dioxygenase and other enzymes capable of converting 2-hydroxy-muconaesemialdehyde into TCA intermediates (Fig.…”

Section: Biodegradation Of Aromatic Compoundsmentioning

confidence: 99%

Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni

Arumugam

Tay

et al. 2015

Appl Microbiol Biotechnol

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Comamonas testosteroni is an important environmental bacterium capable of degrading a variety of toxic aromatic pollutants and has been demonstrated to be a promising biocatalyst for environmental decontamination. This organism is often found to be among the primary surface colonizers in various natural and engineered ecosystems, suggesting an extraordinary capability of this organism in environmental adaptation and biofilm formation. The goal of this study was to gain genetic insights into the adaption of C. testosteroni to versatile environments and the importance of a biofilm lifestyle. Specifically, a draft genome of C. testosteroni I2 was obtained. The draft genome is 5,778,710 bp in length and comprises 110 contigs. The average G+C content was 61.88 %. A total of 5365 genes with 5263 protein-coding genes were predicted, whereas 4324 (80.60 % of total genes) protein-encoding genes were associated with predicted functions. The catabolic genes responsible for biodegradation of steroid and other aromatic compounds on draft genome were identified. Plasmid pI2 was found to encode a complete pathway for aniline degradation and a partial catabolic pathway for chloroaniline. This organism was found to be equipped with a sophisticated signaling system which helps it find ideal niches and switch between planktonic and biofilm lifestyles. A large number of putative multi-drug-resistant genes coding for abundant outer membrane transporters, chaperones, and heat shock proteins for the protection of cellular function were identified in the genome of strain I2. In addition, the genome of strain I2 was predicted to encode several proteins involved in producing, secreting, and uptaking siderophores under iron-limiting conditions. The genome of strain I2 contains a number of genes responsible for the synthesis and secretion of exopolysaccharides, an extracellular component essential for biofilm formation. Overall, our results reveal the genomic features underlying the adaption of C. testosteroni to versatile environments and highlighting the importance of its biofilm lifestyle.

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Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via γ-Glutamylanilide

Cited by 30 publications

References 57 publications

Comparative genomic analysis of isoproturon‐mineralizing sphingomonads reveals the isoproturon catabolic mechanism

Comparative genomic analysis of isoproturon‐mineralizing sphingomonads reveals the isoproturon catabolic mechanism

The many roles of glutamate in metabolism

Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni

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