Mechanisms of polymer degradation in implantable devices. 2. Poly(<scp>DL</scp>‐lactic acid)

Ali, S.A.M.; Doherty, Patrick; Williams, David

doi:10.1002/jbm.820271108

Cited by 107 publications

(44 citation statements)

References 24 publications

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“…[9][10][11][12][13] The copolymer has also been approved for clinical applications owing to its nontoxicity, negligible inflammatory reaction, and biocompatible hydrolysis products (lactic and glycolic acids) after biodegradation. 10,14,15 To prepare biodegradable sheath-core nanofibers, predetermined weight percentages of PLGA, vancomycin, and ceftazidime were dissolved in hexafluoroisopropyl alcohol (HFIP), and rhBMP-2 was dissolved in phosphate-buffered solution. The PLGA/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes with small-diameter needles for coaxial electrospinning.…”

Section: Introductionmentioning

confidence: 99%

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Hsu

Lin²,

et al. 2016

IJN

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In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly( d , l )-lactide- co -glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly( d , l )-lactide- co -glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC] 90 ) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.

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Section: Introductionmentioning

confidence: 99%

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Hsu

Lin²,

et al. 2016

IJN

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“…9,10 The copolymer has also been approved for clinical applications because it is nontoxic, instigates a minimal inflammatory response, and is biodegradable through the hydrolysis of its ester linkage to transform biocompatible lactic and glycolic acids. 11,12 The choice of antibiotics for local delivery is also important. Vancomycin and ceftazidime have been commonly formulated with bone cement for osteomyelitis treatment.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10]17,19 In clinical application, the copolymer is nontoxic, induces minimal inflammatory response, and is biodegraded through hydrolysis without any accumulation in vital organs. [10][11][12] S. aureus is the most common organism implicated in skeletal system infections. 4,20 Approximately 20% of S. aureus isolates collected in Europe are reported to be methicillinresistant S. aureus, and, in US hospitals, the prevalence ranges from 33% to 55%.…”

mentioning

confidence: 99%

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Hsu¹,

Chen²,

Tai³

et al. 2014

IJN

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We developed biodegradable drug-eluting nanofiber-enveloped implants that provided sustained release of vancomycin and ceftazidime. To prepare the biodegradable nanofibrous membranes, poly(D,L)-lactide-co-glycolide and the antibiotics were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol. They were electrospun into biodegradable drug-eluting membranes, which were then enveloped on the surface of stainless plates. An elution method and a high-performance liquid chromatography assay were employed to characterize the in vivo and in vitro release rates of the antibiotics from the nanofiber-enveloped plates. The results showed that the biodegradable nanofiber-enveloped plates released high concentrations of vancomycin and ceftazidime (well above the minimum inhibitory concentration) for more than 3 and 8 weeks in vitro and in vivo, respectively. A bacterial inhibition test was carried out to determine the relative activity of the released antibiotics. The bioactivity ranged from 25% to 100%. In addition, the serum creatinine level remained within the normal range, suggesting that the high vancomycin concentration did not affect renal function. By adopting the electrospinning technique, we will be able to manufacture biodegradable drug-eluting implants for the long-term drug delivery of different antibiotics.

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“…In addition to carrying pharmaceuticals, PLGA is nontoxic, elicits a minimal inflammatory response, and can be eventually absorbed without any accumulation in the vital organs. 22,23 Collagen is a principal structural component of the extracellular matrix. After electrospinning, the morphology of the electrospun nanofibers was characterized by scanning electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

Novel biodegradable sandwich-structured nanofibrous drug-eluting membranes for repair of infected wounds: an in vitro and in vivo study

Chen¹,

Liao²,

Liu³

et al. 2012

IJN

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Background:The purpose of this study was to develop novel sandwich-structured nanofibrous membranes to provide sustained-release delivery of vancomycin, gentamicin, and lidocaine for repair of infected wounds. Methods: To prepare the biodegradable membranes, poly(D, L)-lactide-co-glycolide (PLGA), collagen, and various pharmaceuticals, including vancomycin, gentamicin, and lidocaine, were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol. They were electrospun into sandwichstructured membranes with PLGA/collagen as the surface layers and PLGA/drugs as the core. An elution method and a high-pressure liquid chromatography assay were used to characterize in vivo and in vitro drug release from the membranes. In addition, repair of infected wounds in rats was studied. Histological examination of epithelialization and granulation at the wound site was also performed. Results: The biodegradable nanofibrous membranes released large amounts of vancomycin and gentamicin (well above the minimum inhibition concentration) and lidocaine in vivo for more than 3 weeks. A bacterial inhibition test was carried out to determine the relative activity of the antibiotics released. The bioactivity ranged from 40% to 100%. The nanofibrous membranes were functionally active in treating infected wounds, and were very effective as accelerators in early-stage wound healing. Conclusion: Using the electrospinning technique, we will be able to manufacture biodegradable, biomimetic, nanofibrous, extracellular membranes for long-term delivery of various drugs.

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Mechanisms of polymer degradation in implantable devices. 2. Poly(DL‐lactic acid)

Cited by 107 publications

References 24 publications

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Novel biodegradable sandwich-structured nanofibrous drug-eluting membranes for repair of infected wounds: an in vitro and in vivo study

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