Mechanisms of lipid metabolism promoted by berberine via peroxisome proliferator‐activated receptor gamma during in vitro maturation of porcine oocytes

Dai, Jia‐Ge; Huang, Xiaomeng; Zhang, Chao; Gao, Jianming

doi:10.1111/asj.13582

Cited by 6 publications

(4 citation statements)

References 53 publications

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“…During reproduction, oocyte maturation quality is important for successful fertilization and embryo development [24]. The cumulus cell expansion and polar body extrusion are regarded as two important indicators of porcine oocyte maturation [25].…”

Section: Discussionmentioning

confidence: 99%

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

Chen

Wang

Pan

et al. 2022

J Animal Sci Biotechnol

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Background Recently, defects in mammalian oocytes maturation induced by environmental pollution results in the decreasing animal reproduction. Animal exposed to glyphosate is largely unavoidable because glyphosate is one of the most widely used herbicide worldwide due to its high-efficiency and broad-spectrum effects, which causes glyphosate an environmental contaminant found in soil, water and food. During the last few years, the growing and wider use of glyphosate has raised great concerns about its effects of reproductive toxicity. In this study, using porcine models, we investigated effects of glyphosate on organelle functions during oocyte meiosis. Results The results showed glyphosate exposure disrupted porcine oocyte maturation. Expression levels of cumulus expansion-related genes were interfered, further indicating the meiotic defects. The damaging effects were mediated by destruction of mitochondrial distribution and functions, which induced ROS accumulation and oxidative stress, also indicated by the decreased mRNA expression of related antioxidant enzyme genes. We also found an interference of endoplasmic reticulum (ER) distribution, disturbance of Ca2+ homeostasis, as well as fluctuation of ER stress, showing with the reduced ER stress-related mRNA or protein expression, which could indicate the dysfunction of ER for protein processing and signal transduction in glyphosate-exposed oocytes. Moreover, glyphosate exposure induced the disruption of lysosome function for autophagy, showing with the decrease of LAMP2 expression and autophagy-related genes mRNA expression. Additionally, our data showed the distribution of Golgi apparatus and the functions of ribosome were disturbed after glyphosate exposure, which might affect protein synthesis and transport. Conclusions Collectively, our study showed that exposed to glyphosate could affect animal reproduction by compromising the quality of oocytes through its wide toxic effects on organelle functions.

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Section: Discussionmentioning

confidence: 99%

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

Chen

Wang

Pan

et al. 2022

J Animal Sci Biotechnol

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“…Oocyte maturation quality is vital for successful fertilization and embryo development during bovine reproduction ( Dai et al, 2021 ). The polar body extrusion and embryo development potential are regarded as two critical indicators for oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

β-hydroxybutyrate impairs bovine oocyte maturation via pyruvate dehydrogenase (PDH) associated energy metabolism abnormality

et al. 2023

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Background: Ketosis is one of the most frequent and costly metabolic disorders in high-producing dairy cows, and negatively associated with the health and reproductive performance of bovine. Ketosis is mainly caused by the accumulation of ketone body β-hydroxybutyric acid and its diagnosis is based on β-hydroxybutyrate (βHB) concentration in blood.Methods: In this study, we investigated the effects of βHB on bovine oocyte maturation in the concentration of subclinical (1.2 mM) βHB and clinical (3.6 mM).Results: The results showed βHB disrupted bovine oocyte maturation and development capacity. Further analysis showed that βHB induced oxidative stress and mitochondrial dysfunction, as indicated by the increased level of reactive oxygen species (ROS), disrupted mitochondrial structure and distribution, and depolarized membrane potential. Furthermore, oxidative stress triggered early apoptosis, as shown by the enhanced levels of Caspase-3 and Annexin-V. Moreover, 3.6 mM βHB induced the disruption of the pyruvate dehydrogenase (PDH) activity, showing with the decrease of the global acetylation modification and the increase of the abnormal spindle rate.Conclusion: Our study showed that βHB in subclinical/clinical concentration had toxic effects on mitochondrial function and PDH activity, which might affect energy metabolism and epigenetic modification of bovine oocytes and embryos.

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“…The SREBF1 proteins are crucial in regulating lipid metabolism (Shimano and Sato 2017). Berberine promotes lipid metabolism in porcine oocytes by down-regulating SREBF1 and peroxisome proliferator-activated receptor gamma (PPARG), thus improving the IVM of porcine oocytes (Dai et al 2021). In the present study, the expression level of Srebf1 mRNA increased as LR-BSA increased.…”

Section: Discussionmentioning

confidence: 99%

Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst

Kwon,

Park,

HakKyo

et al. 2023

CZECH J ANIM SCI

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Lipid-rich bovine serum albumin (LR-BSA) has been reported to increase the survival rate of blastocysts after freezing and thawing. However studies on the early development of in vitro fertilised (IVF) embryos are still insufficient in cattle. This study investigated the blastocyst productivity of in vitro culture (IVC) medium (synthetic oviductal fluid medium with amino acids, mSOFaa) supplemented with 5% fetal bovine serum (FBS, Control) or LR-BSA (1, 2.5, 5 mg/ml dosages, respectively) and the qualitative characteristics of produced blastocysts. Cleavage rates on day 2 were similar between all groups. Significant differences were obtained in the blastocyst rate in the 2.5LR-BSA group compared with the control (23.5% vs 35.7%, P < 0.05). Hatching rates were significantly higher in all treatment groups than in control (P < 0.05). The cell number in blastocysts did not differ among groups, but the apoptotic index in the 5.0LR-BSA group was higher than the control (P < 0.05). The expression of embryo quality-related markers was affected. Octamer-binding transcription factor 4 (Oct4), placenta associated 8 (Plac8), and sex-determining region Y-box 2 (Sox2) genes expression in the 2.5LR-BSA group were significantly up-regulated compared to the control (P < 0.05). Cytoplasmic lipid contents and the lipid droplet formation-related gene, sterol regulatory element binding transcription factor 1 (Srebf1), were significantly increased in the 5.0LR-BSA group (P < 0.05). Therefore, the LR-BSA supplementation (2.5 mg/ml) to mSOFaa medium could contribute to the early development of bovine IVF embryos and the qualitative improvement of the resulting blastocysts.

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Mechanisms of lipid metabolism promoted by berberine via peroxisome proliferator‐activated receptor gamma during in vitro maturation of porcine oocytes

Cited by 6 publications

References 53 publications

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

β-hydroxybutyrate impairs bovine oocyte maturation via pyruvate dehydrogenase (PDH) associated energy metabolism abnormality

Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst

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