Mechanisms of Inactivation of Single-Stranded Virus Nucleic Acids by Heat

Ginoza, William; Hoelle, Carol J.; Vessey, Kristin B.; Carmack, C

doi:10.1038/203606a0

Cited by 35 publications

(11 citation statements)

References 23 publications

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“…In accordance with these observations L-cystine protects poliovirus completely against inactivation at 500 C., very poorly at 370 C. and not at all at 320 C. (Pohjanpelto, 1962). Ginoza et al (1964) with TMV and phage R 17; (3) Woese (1960); (4) Dimmock (1967).…”

Section: Discussionsupporting

confidence: 83%

The inactivation of poliovirus in aerosols

Jong

Winkler

1968

Epidemiol. Infect.

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Poliovirus type 1, strain LSc2ab, was directly sprayed in a static air cabinet at 20° C. and sampled afterwards with impingers. Virus inactivation during spraying was maximal in dry air; during storage maximal decay occurred at moderate humidity.With regard to the mechanism of the inactivation the following facts have been observed: (1) The RNA in the virus decays simultaneously with the whole virus particle. (2) Oxidation does not play any significant role. (3) Incubation with L-cystine protects the virus against inactivation at 50° C. in aqueous suspension, but not against decay in aerosols.On the strength of these observations it is concluded that in aerosols denatura-tion of the viral RNA is the cause of the inactivation of poliovirus. The decay in aerosols has some features in common with the thermal inactivation in aqueous suspensions at room temperature.

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Section: Discussionsupporting

confidence: 83%

The inactivation of poliovirus in aerosols

Jong

Winkler

1968

Epidemiol. Infect.

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“…The mechanisms underlying heat inactivation of single-stranded viruses have been discussed by Ginoza et al [26], and Nuanualsuawan and Cliver [27] have addressed the mechanisms for the caliciviruses and picornaviruses in particular. A more recent discussion of mechanisms of heat inactivation of viruses of importance to food protection, including caliciviruses, can be found in Hirneisen et al [28].…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Caliciviruses

Nims¹,

Plavsic²

2013

Pharmaceuticals

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The Caliciviridae family of viruses contains clinically important human and animal pathogens, as well as vesivirus 2117, a known contaminant of biopharmaceutical manufacturing processes employing Chinese hamster cells. An extensive literature exists for inactivation of various animal caliciviruses, especially feline calicivirus and murine norovirus. The caliciviruses are susceptible to wet heat inactivation at temperatures in excess of 60 °C with contact times of 30 min or greater, to UV-C inactivation at fluence ≥30 mJ/cm2, to high pressure processing >200 MPa for >5 min at 4 °C, and to certain photodynamic inactivation approaches. The enteric caliciviruses (e.g.; noroviruses) display resistance to inactivation by low pH, while the non-enteric species (e.g.; feline calicivirus) are much more susceptible. The caliciviruses are inactivated by a variety of chemicals, including alcohols, oxidizing agents, aldehydes, and β-propiolactone. As with inactivation of viruses in general, inactivation of caliciviruses by the various approaches may be matrix-, temperature-, and/or contact time-dependent. The susceptibilities of the caliciviruses to the various physical and chemical inactivation approaches are generally similar to those displayed by other small, non-enveloped viruses, with the exception that the parvoviruses and circoviruses may require higher temperatures for inactivation, while these families appear to be more susceptible to UV-C inactivation than are the caliciviruses.

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“…In circular, single-stranded DNA, such as that of bacteriophage j6X174:, one backbone scission converts Spectrophotometric loss of absorption at 260 m", Thymine version can be followed easily as a function of radiation dose. Though not yet studied by centrifuge methods for radiation experiments, this viral DNA is known to be inactivated by a single enzyme-i nduced scission (85) or by a single depurination event (86). By contrast, a radiation-induced, single-strand scission in a linear double stranded DNA remains hidden because of the cooperative hydrogen bonding between the two polynucleotide strands.…”

Section: Radiation Polymer Chemistry Of Nucleic Acidsmentioning

confidence: 97%

The Effects of Ionizing Radiation on Nucleic Acids of Bacteriophages and Bacterial Cells

Ginoza¹

1967

Annu. Rev. Microbiol.

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Mechanisms of Inactivation of Single-Stranded Virus Nucleic Acids by Heat

Cited by 35 publications

References 23 publications

The inactivation of poliovirus in aerosols

The inactivation of poliovirus in aerosols

Inactivation of Caliciviruses

The Effects of Ionizing Radiation on Nucleic Acids of Bacteriophages and Bacterial Cells

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