Mechanisms of Gastric Mucus Secretion from Cultured Rat Gastric Epithelial Cells Induced by Carbachol, Cholecystokinin Octapeptide, Secretin, and Prostaglandin E2.

Tani, Shigemasa; Suzuki, Teruo; Kano, Sayuri; Tanaka, Toru; Sunaga, K.; Morishige, Rie; Tsuda, Tadashi

doi:10.1248/bpb.25.14

Cited by 15 publications

(10 citation statements)

References 7 publications

(4 reference statements)

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“…Thus, CCK8 (but also secretin, carbachol and PG E2) strongly stimulated mucus secretion from cultured rat gastric epithelial cells and gastrin stimulated it weakly (Tani et al 2002). It seems likely that mucus secretion results from the existence of interaction among receptors (Tani et al 2002).…”

Section: Local Effects Of Cholecystokinin and Gastrin In The Gutmentioning

confidence: 90%

Gastrin, cholecystokinin and gastrointestinal tract functions in mammals

et al. 2006

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The aim of the present review is to synthesise and summarise our recent knowledge on the involvement of cholecystokinin (CCK) and gastrin peptides and their receptors in the control of digestive functions and more generally their role in the field of nutrition in mammals. First, we examined the release of these peptides from the gut, focusing on their molecular forms, the factors regulating their release and the signalling pathways mediating their effects. Second, general physiological effects of CCK and gastrin peptides are described with regard to their specific receptors and the role of CCK on vagal mucosal afferent nerve activities. Local effects of CCK and gastrin in the gut are also reported, including gut development, gastrointestinal motility and control of pancreatic functions through vagal afferent pathways, including NO. Third, some examples of the intervention of the CCK and gastrin peptides are exposed in diseases, taking into account intervention of the classical receptor subtypes (CCK 1 and CCK 2 receptors) and their heterodimerisation as well as CCK-C receptor subtype. Finally, applications and future challenges are suggested in the nutritional field (performances) and in therapy with regards to the molecular forms or in relation with the type of receptor as well as new techniques to be utilised in detection or in therapy of disease. In conclusion, the present review underlines recent developments in this field: CCK and gastrin peptides and their receptors are the key factor of nutritional aspects; a better understanding of the mechanisms involved may increase the efficiency of the nutritional functions and the treatment of abnormalities under pathological conditions.

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Section: Local Effects Of Cholecystokinin and Gastrin In The Gutmentioning

confidence: 90%

Gastrin, cholecystokinin and gastrointestinal tract functions in mammals

et al. 2006

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“…Isolation of primary cells from the epithelium of mouse stomach and ELLA were performed as previously described (Tani et al . 2002) with slight modifications.…”

Section: Methodsmentioning

confidence: 99%

Decreased basal mucus secretion by Slp2‐a‐deficient gastric surface mucous cells

et al. 2006

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Synaptotagmin-like protein (Slp) 2-a is a putative Rab27A/B-effector protein and is implicated in intracellular membrane transport. However, the precise tissue distribution of Slp2-a protein and its functions remain largely unknown. In this study we used a specific anti-Slp2-a antibody to investigate the tissue distribution of Slp2-a in mice and found that Slp2-a is most abundantly expressed in mouse stomach. Co-immunoprecipitation experiments indicated that Slp2-a interacts with Rab27A/B in vivo . We also discovered that Slp2-a and Rab27A/B are predominantly localized at the apical region of gastric-surface mucous cells, where mucus granules are accumulated. Analysis of Slp2-a mutant mice generated by homologous recombination showed a reduced number of mucus granules, a deficiency of granule docking with the apical plasma membrane in the gastric-surface mucous cells and reduction of mucus secretion by Slp2-a-deficient gastric primary cells. Based on these results, we propose that Slp2-a is part of the mucin secretory machinery in surface mucous cells of mouse stomach.

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“…Exposure to indomethacin or AA modulates the synthesis of prostaglandins. Prostaglandins have an essential role in gastric mucosal protection because the secretion of mucus depends on prostaglandin E 2 [33].…”

Section: Discussionmentioning

confidence: 99%

Heat Shock Preconditioning Induces Protein Carbonylation and Alters Antioxidant Protection in Superficially Injured Guinea Pig Gastric Mucosa In Vitro

et al. 2007

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According to our previous studies, heat shock preconditioning of gastric mucosa requires modulation of protein synthesis and eicosanoid pathways to induce protection against superficial injury. This may be caused by heat shock-induced oxidative stress. We studied the effect of heat shock preconditioning with normothermic recovery on redox status in superficially injured (1.25 mmol NaCl for 5 min) Ussing chamber perfused guinea pig gastric mucosa allowed to recover for 3 hr after injury. Protein oxidation, lipid peroxidation, level of superoxide dismutase, level of heat shock protein 72 (HSP72), and level of oxygen radical absorbance capacity were measured. Superficial injury increased lipid peroxidation. Heat shock preconditioning decreased oxygen radical absorbance capacity and increased protein carbonyl and HSP72 levels, but inhibited electrophysiologic recovery. Exposure to indomethacin and arachidonic acid (AA) partially abolished this pro-oxidative and inhibitory effect on recovery, but maintained HSP72 levels and decreased protein carbonyls, lipid peroxidation, and oxygen radical absorbance capacity. In conclusion, superficial injury increased lipid peroxidation. Heat shock preconditioning alone induced oxidative stress via indomethacin- and AA-sensitive mechanisms. The development of optimal cytoprotective strategy may therefore require control of oxidative stress and modulation of the eicosanoid pathways.

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Mechanisms of Gastric Mucus Secretion from Cultured Rat Gastric Epithelial Cells Induced by Carbachol, Cholecystokinin Octapeptide, Secretin, and Prostaglandin E2.

Cited by 15 publications

References 7 publications

Gastrin, cholecystokinin and gastrointestinal tract functions in mammals

Gastrin, cholecystokinin and gastrointestinal tract functions in mammals

Decreased basal mucus secretion by Slp2‐a‐deficient gastric surface mucous cells

Heat Shock Preconditioning Induces Protein Carbonylation and Alters Antioxidant Protection in Superficially Injured Guinea Pig Gastric Mucosa In Vitro

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