Heat Shock Preconditioning Induces Protein Carbonylation and Alters Antioxidant Protection in Superficially Injured Guinea Pig Gastric Mucosa In Vitro

Oksala, Niku; Paimela, Hannu; Alhava, Esko; Atalay, Mustafa

doi:10.1007/s10620-006-9214-1

Cited by 10 publications

(7 citation statements)

References 42 publications

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“…To evaluate the potential effect of diabetes or exercise training on markers of oxidative stress, protein carbonyls were measured using an ELISA method, as described previously (Oksala et al, 2007b), in brain tissue homogenates. The carbonyl contents are expressed as nmol of protein carbonyl in mg of total protein (nmol/mg protein).…”

Section: Methodsmentioning

confidence: 99%

Exercise training and experimental diabetes modulate heat shock protein response in brain

Lappalainen

Oksala

et al. 2010

Scandinavian Med Sci Sports

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In diabetes, defense systems against cellular stress are impaired. Heat shock proteins (HSPs) function primarily as molecular chaperones. Factors that raise tissue HSP levels may slow progression of diabetes and improve diabetic complications that also affect brain tissue. This study tested the effect of an 8-week exercise training on brain HSP response in rats with or without streptozotocin-induced diabetes (SID). In untrained animals, the HSP levels were not different between SID and non-diabetic groups. Endurance training, however, increased HSP72 and HSP90 protein in non-diabetic rats, whereas SID significantly decreased the effect of training on these HSPs. At the mRNA level, HSP60, HSP90 and GRP75 were increased due to training, whereas HSP72 mRNA was only increased in exercise-trained diabetic animals. Training or diabetes had no effect on protein carbonyl content, a marker of oxidative damage. Altogether, our findings suggest that endurance training increases HSP expression in the brain, and that experimental diabetes is associated with an incomplete HSP response at the protein level.

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Section: Methodsmentioning

confidence: 99%

Exercise training and experimental diabetes modulate heat shock protein response in brain

Lappalainen

Oksala

et al. 2010

Scandinavian Med Sci Sports

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“…A blank for DNP reagent in PBS without protein was subtracted from all other absorbances. A 6-point standard curve of reduced and oxidised BSA was included with each plate [21] .…”

Section: Carbonyl Analysesmentioning

confidence: 99%

Validation of protein carbonyl measurement: A multi-centre study

et al. 2015

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Protein carbonyls are widely analysed as a measure of protein oxidation. Several different methods exist for their determination. A previous study had described orders of magnitude variance that existed when protein carbonyls were analysed in a single laboratory by ELISA using different commercial kits. We have further explored the potential causes of variance in carbonyl analysis in a ring study. A soluble protein fraction was prepared from rat liver and exposed to 0, 5 and 15 min of UV irradiation. Lyophilised preparations were distributed to six different laboratories that routinely undertook protein carbonyl analysis across Europe. ELISA and Western blotting techniques detected an increase in protein carbonyl formation between 0 and 5 min of UV irradiation irrespective of method used. After irradiation for 15 min, less oxidation was detected by half of the laboratories than after 5 min irradiation. Three of the four ELISA carbonyl results fell within 95% confidence intervals. Likely errors in calculating absolute carbonyl values may be attributed to differences in standardisation. Out of up to 88 proteins identified as containing carbonyl groups after tryptic cleavage of irradiated and control liver proteins, only seven were common in all three liver preparations. Lysine and arginine residues modified by carbonyls are likely to be resistant to tryptic proteolysis. Use of a cocktail of proteases may increase the recovery of oxidised peptides. In conclusion, standardisation is critical for carbonyl analysis and heavily oxidised proteins may not be effectively analysed by any existing technique.

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“…Plasma samples were stored in multiple portions at -80°C until analysis. Protein carbonyls, markers of protein oxidative damage, were measured using an ELISA method as previously described (19). Nitrotyrosine concentrations were determined with the ELISA method using a commercial kit (Hycult Biotechnology BV, Uden, The Netheriands).…”

Section: Subjectsmentioning

confidence: 99%